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DNA barcoding data release for the Phoridae (Insecta, Diptera) of the Halimun-Salak National Park (Java, Indonesia)

Launched in 2015, the large-scale initiative Indonesian Biodiversity Discovery and Information System (IndoBioSys) is a multidisciplinary German-Indonesian collaboration with the main goal of establishing a standardised framework for species discovery and all associated steps. One aspect of the proj...

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Autores principales: Chimeno, Caroline, Schmidt, Stefan, Hamid, Hasmiandy, Narakusumo, Raden Pramesa, Peggie, Djunijanti, Balke, Michael, Cancian de Araujo, Bruno
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pensoft Publishers 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10336553/
https://www.ncbi.nlm.nih.gov/pubmed/37448693
http://dx.doi.org/10.3897/BDJ.11.e104942
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author Chimeno, Caroline
Schmidt, Stefan
Hamid, Hasmiandy
Narakusumo, Raden Pramesa
Peggie, Djunijanti
Balke, Michael
Cancian de Araujo, Bruno
author_facet Chimeno, Caroline
Schmidt, Stefan
Hamid, Hasmiandy
Narakusumo, Raden Pramesa
Peggie, Djunijanti
Balke, Michael
Cancian de Araujo, Bruno
author_sort Chimeno, Caroline
collection PubMed
description Launched in 2015, the large-scale initiative Indonesian Biodiversity Discovery and Information System (IndoBioSys) is a multidisciplinary German-Indonesian collaboration with the main goal of establishing a standardised framework for species discovery and all associated steps. One aspect of the project includes the application of DNA barcoding for species identification and biodiversity assessments. In this framework, we conducted a large-scale assessment of the insect fauna of the Mount Halimun-Salak National Park which is one of the largest tropical rain-forest ecosystems left in West Java. In this study, we present the results of processing 5,034 specimens of Phoridae (scuttle flies) via DNA barcoding. Despite limited sequencing success, we obtained more than 500 clusters using different algorithms (RESL, ASAP, SpeciesIdentifier). Moreover, Chao statistics indicated that we drastically undersampled all trap sites, implying that the true diversity of Phoridae is, in fact, much higher. With this data release, we hope to shed some light on the hidden diversity of this megadiverse group of flies.
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spelling pubmed-103365532023-07-13 DNA barcoding data release for the Phoridae (Insecta, Diptera) of the Halimun-Salak National Park (Java, Indonesia) Chimeno, Caroline Schmidt, Stefan Hamid, Hasmiandy Narakusumo, Raden Pramesa Peggie, Djunijanti Balke, Michael Cancian de Araujo, Bruno Biodivers Data J Research Article Launched in 2015, the large-scale initiative Indonesian Biodiversity Discovery and Information System (IndoBioSys) is a multidisciplinary German-Indonesian collaboration with the main goal of establishing a standardised framework for species discovery and all associated steps. One aspect of the project includes the application of DNA barcoding for species identification and biodiversity assessments. In this framework, we conducted a large-scale assessment of the insect fauna of the Mount Halimun-Salak National Park which is one of the largest tropical rain-forest ecosystems left in West Java. In this study, we present the results of processing 5,034 specimens of Phoridae (scuttle flies) via DNA barcoding. Despite limited sequencing success, we obtained more than 500 clusters using different algorithms (RESL, ASAP, SpeciesIdentifier). Moreover, Chao statistics indicated that we drastically undersampled all trap sites, implying that the true diversity of Phoridae is, in fact, much higher. With this data release, we hope to shed some light on the hidden diversity of this megadiverse group of flies. Pensoft Publishers 2023-07-04 /pmc/articles/PMC10336553/ /pubmed/37448693 http://dx.doi.org/10.3897/BDJ.11.e104942 Text en Caroline Chimeno, Stefan Schmidt, Hasmiandy Hamid, Raden Pramesa Narakusumo, Djunijanti Peggie, Michael Balke, Bruno Cancian de Araujo https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Chimeno, Caroline
Schmidt, Stefan
Hamid, Hasmiandy
Narakusumo, Raden Pramesa
Peggie, Djunijanti
Balke, Michael
Cancian de Araujo, Bruno
DNA barcoding data release for the Phoridae (Insecta, Diptera) of the Halimun-Salak National Park (Java, Indonesia)
title DNA barcoding data release for the Phoridae (Insecta, Diptera) of the Halimun-Salak National Park (Java, Indonesia)
title_full DNA barcoding data release for the Phoridae (Insecta, Diptera) of the Halimun-Salak National Park (Java, Indonesia)
title_fullStr DNA barcoding data release for the Phoridae (Insecta, Diptera) of the Halimun-Salak National Park (Java, Indonesia)
title_full_unstemmed DNA barcoding data release for the Phoridae (Insecta, Diptera) of the Halimun-Salak National Park (Java, Indonesia)
title_short DNA barcoding data release for the Phoridae (Insecta, Diptera) of the Halimun-Salak National Park (Java, Indonesia)
title_sort dna barcoding data release for the phoridae (insecta, diptera) of the halimun-salak national park (java, indonesia)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10336553/
https://www.ncbi.nlm.nih.gov/pubmed/37448693
http://dx.doi.org/10.3897/BDJ.11.e104942
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