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Altered expression of apoptosis‐related genes in rheumatoid arthritis peripheral blood mononuclear cell and related miRNA regulation

AIM: Impaired apoptosis and proliferation resulted in autoreactive lymphocyte development and inflammation in Rheumatoid arthritis (RA). TP53, BAX, FOXO1, and RB1 are related genes in cell survival, proliferation, and inflammation which could be important in RA development and disease severity. Here...

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Autores principales: Ebrahimian, Hamidreza, Akhtari, Maryam, Akhlaghi, Maassoumeh, Farhadi, Elham, Jamshidi, Ahmadreza, Alishiri, Gholam Hossein, Mahmoudi, Mahdi, Tavallaie, Mahmood
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10336681/
https://www.ncbi.nlm.nih.gov/pubmed/37506143
http://dx.doi.org/10.1002/iid3.914
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author Ebrahimian, Hamidreza
Akhtari, Maryam
Akhlaghi, Maassoumeh
Farhadi, Elham
Jamshidi, Ahmadreza
Alishiri, Gholam Hossein
Mahmoudi, Mahdi
Tavallaie, Mahmood
author_facet Ebrahimian, Hamidreza
Akhtari, Maryam
Akhlaghi, Maassoumeh
Farhadi, Elham
Jamshidi, Ahmadreza
Alishiri, Gholam Hossein
Mahmoudi, Mahdi
Tavallaie, Mahmood
author_sort Ebrahimian, Hamidreza
collection PubMed
description AIM: Impaired apoptosis and proliferation resulted in autoreactive lymphocyte development and inflammation in Rheumatoid arthritis (RA). TP53, BAX, FOXO1, and RB1 are related genes in cell survival, proliferation, and inflammation which could be important in RA development and disease severity. Here we investigated their expression in peripheral blood mononuclear cells (PBMCs) from RA patients in comparison to healthy controls. METHODS: Fifty healthy controls and 50 RA patients were selected. The quantitative real‐time polymerase chain reaction was used to assess the gene expression level in PBMCs. RESULTS: The mRNA expression of TP53 (FC = 0.65, p = .000), BAX (FC = 0.76, p = .008), FOXO1 (FC = 0.59, p = .000) and RB1 (FC = 0.50, p = .000) were significantly reduced in RA PBMCs. TP53 expression was negatively correlated with miR‐16‐5p (p = .032) and FOXO1 expression was negatively correlated with miR‐335‐5p (p = .005) and miR‐34a‐5p (p = .014). A positive correlation was seen between TP53 expression and its downstream gene, BAX (p = .001). FOXO1 expression was also negatively correlated with disease activity, DAS28 (p = .021). CONCLUSION: All selected genes have downregulated expression in RA PBMCs which could be correlated with RA pathogenesis by regulating apoptosis, cell survival, inflammatory mediator production, and proliferation. Due to the correlation of miR‐16‐5p, miR‐34a‐5p, and miR‐335‐5p with TP53 and FOXO1 expression in RA PBMCs, they could be used as future therapeutic targets.
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spelling pubmed-103366812023-07-13 Altered expression of apoptosis‐related genes in rheumatoid arthritis peripheral blood mononuclear cell and related miRNA regulation Ebrahimian, Hamidreza Akhtari, Maryam Akhlaghi, Maassoumeh Farhadi, Elham Jamshidi, Ahmadreza Alishiri, Gholam Hossein Mahmoudi, Mahdi Tavallaie, Mahmood Immun Inflamm Dis Original Articles AIM: Impaired apoptosis and proliferation resulted in autoreactive lymphocyte development and inflammation in Rheumatoid arthritis (RA). TP53, BAX, FOXO1, and RB1 are related genes in cell survival, proliferation, and inflammation which could be important in RA development and disease severity. Here we investigated their expression in peripheral blood mononuclear cells (PBMCs) from RA patients in comparison to healthy controls. METHODS: Fifty healthy controls and 50 RA patients were selected. The quantitative real‐time polymerase chain reaction was used to assess the gene expression level in PBMCs. RESULTS: The mRNA expression of TP53 (FC = 0.65, p = .000), BAX (FC = 0.76, p = .008), FOXO1 (FC = 0.59, p = .000) and RB1 (FC = 0.50, p = .000) were significantly reduced in RA PBMCs. TP53 expression was negatively correlated with miR‐16‐5p (p = .032) and FOXO1 expression was negatively correlated with miR‐335‐5p (p = .005) and miR‐34a‐5p (p = .014). A positive correlation was seen between TP53 expression and its downstream gene, BAX (p = .001). FOXO1 expression was also negatively correlated with disease activity, DAS28 (p = .021). CONCLUSION: All selected genes have downregulated expression in RA PBMCs which could be correlated with RA pathogenesis by regulating apoptosis, cell survival, inflammatory mediator production, and proliferation. Due to the correlation of miR‐16‐5p, miR‐34a‐5p, and miR‐335‐5p with TP53 and FOXO1 expression in RA PBMCs, they could be used as future therapeutic targets. John Wiley and Sons Inc. 2023-07-12 /pmc/articles/PMC10336681/ /pubmed/37506143 http://dx.doi.org/10.1002/iid3.914 Text en © 2023 The Authors. Immunity, Inflammation and Disease published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Ebrahimian, Hamidreza
Akhtari, Maryam
Akhlaghi, Maassoumeh
Farhadi, Elham
Jamshidi, Ahmadreza
Alishiri, Gholam Hossein
Mahmoudi, Mahdi
Tavallaie, Mahmood
Altered expression of apoptosis‐related genes in rheumatoid arthritis peripheral blood mononuclear cell and related miRNA regulation
title Altered expression of apoptosis‐related genes in rheumatoid arthritis peripheral blood mononuclear cell and related miRNA regulation
title_full Altered expression of apoptosis‐related genes in rheumatoid arthritis peripheral blood mononuclear cell and related miRNA regulation
title_fullStr Altered expression of apoptosis‐related genes in rheumatoid arthritis peripheral blood mononuclear cell and related miRNA regulation
title_full_unstemmed Altered expression of apoptosis‐related genes in rheumatoid arthritis peripheral blood mononuclear cell and related miRNA regulation
title_short Altered expression of apoptosis‐related genes in rheumatoid arthritis peripheral blood mononuclear cell and related miRNA regulation
title_sort altered expression of apoptosis‐related genes in rheumatoid arthritis peripheral blood mononuclear cell and related mirna regulation
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10336681/
https://www.ncbi.nlm.nih.gov/pubmed/37506143
http://dx.doi.org/10.1002/iid3.914
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