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Toward the use of mixed microbial cultures for the biological production of adipic and levulinic acid

Biological synthesis of high added-value compounds like adipic acid (AA), levulinic acid (LA), or polyhydroxybutyrate (PHB) using pure culture has been separately reported. However, pure culture requires sterile conditions and the use of specific carbon sources resulting in high operating costs. Dif...

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Autores principales: Pinto-Ibieta, Fernanda, Cea, Mara, Serrano, Antonio, Felissia, Fernando E., Area, María Cristina, Cabrera, Francisco, Ciudad, Gustavo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10338001/
https://www.ncbi.nlm.nih.gov/pubmed/37448576
http://dx.doi.org/10.3389/fmicb.2023.1224543
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author Pinto-Ibieta, Fernanda
Cea, Mara
Serrano, Antonio
Felissia, Fernando E.
Area, María Cristina
Cabrera, Francisco
Ciudad, Gustavo
author_facet Pinto-Ibieta, Fernanda
Cea, Mara
Serrano, Antonio
Felissia, Fernando E.
Area, María Cristina
Cabrera, Francisco
Ciudad, Gustavo
author_sort Pinto-Ibieta, Fernanda
collection PubMed
description Biological synthesis of high added-value compounds like adipic acid (AA), levulinic acid (LA), or polyhydroxybutyrate (PHB) using pure culture has been separately reported. However, pure culture requires sterile conditions and the use of specific carbon sources resulting in high operating costs. Different alternatives based on the use of mixed microbial cultures (MMC) have been explored to resolve this problem. MMC have been widely reported for the production of PHB, but scarcely reported for LA production and never for AA synthesis. This work presents a novel strategy for the co-production of AA LA, and PHB using MMC. The strategy consists in selecting an MMC producer of AA, LA and PHB from an inoculum obtained from a wastewater treatment plant, which is then subjected to the feast and famine culture strategy in a sequential batch reactor, coupled with a batch reactor step to enhance the accumulation of AA and LA. The results showed that the MMC could produce a 16 ± 2, 23 ± 1 and 5 ± %1 (g compound/g volatile solids) of AA, LA and PHB, respectively, using a non-fermented residual biomass rich in pentose, namely synthetic hemicellulose hydrolysate (SHH) as the carbon source. These results contribute to generating future research to better understand and optimise the biosynthesis of these compounds by MMC.
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spelling pubmed-103380012023-07-13 Toward the use of mixed microbial cultures for the biological production of adipic and levulinic acid Pinto-Ibieta, Fernanda Cea, Mara Serrano, Antonio Felissia, Fernando E. Area, María Cristina Cabrera, Francisco Ciudad, Gustavo Front Microbiol Microbiology Biological synthesis of high added-value compounds like adipic acid (AA), levulinic acid (LA), or polyhydroxybutyrate (PHB) using pure culture has been separately reported. However, pure culture requires sterile conditions and the use of specific carbon sources resulting in high operating costs. Different alternatives based on the use of mixed microbial cultures (MMC) have been explored to resolve this problem. MMC have been widely reported for the production of PHB, but scarcely reported for LA production and never for AA synthesis. This work presents a novel strategy for the co-production of AA LA, and PHB using MMC. The strategy consists in selecting an MMC producer of AA, LA and PHB from an inoculum obtained from a wastewater treatment plant, which is then subjected to the feast and famine culture strategy in a sequential batch reactor, coupled with a batch reactor step to enhance the accumulation of AA and LA. The results showed that the MMC could produce a 16 ± 2, 23 ± 1 and 5 ± %1 (g compound/g volatile solids) of AA, LA and PHB, respectively, using a non-fermented residual biomass rich in pentose, namely synthetic hemicellulose hydrolysate (SHH) as the carbon source. These results contribute to generating future research to better understand and optimise the biosynthesis of these compounds by MMC. Frontiers Media S.A. 2023-06-28 /pmc/articles/PMC10338001/ /pubmed/37448576 http://dx.doi.org/10.3389/fmicb.2023.1224543 Text en Copyright © 2023 Pinto-Ibieta, Cea, Serrano, Felissia, Area, Cabrera and Ciudad. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Pinto-Ibieta, Fernanda
Cea, Mara
Serrano, Antonio
Felissia, Fernando E.
Area, María Cristina
Cabrera, Francisco
Ciudad, Gustavo
Toward the use of mixed microbial cultures for the biological production of adipic and levulinic acid
title Toward the use of mixed microbial cultures for the biological production of adipic and levulinic acid
title_full Toward the use of mixed microbial cultures for the biological production of adipic and levulinic acid
title_fullStr Toward the use of mixed microbial cultures for the biological production of adipic and levulinic acid
title_full_unstemmed Toward the use of mixed microbial cultures for the biological production of adipic and levulinic acid
title_short Toward the use of mixed microbial cultures for the biological production of adipic and levulinic acid
title_sort toward the use of mixed microbial cultures for the biological production of adipic and levulinic acid
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10338001/
https://www.ncbi.nlm.nih.gov/pubmed/37448576
http://dx.doi.org/10.3389/fmicb.2023.1224543
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