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Distinct inflammatory signatures of upper and lower body adipose tissue and adipocytes in women with normal weight or obesity

INTRODUCTION: Upper and lower body fat accumulation poses an opposing obesity-related cardiometabolic disease risk. Depot-differences in subcutaneous adipose tissue (SAT) function may underlie these associations. We aimed to investigate the inflammatory signatures of abdominal (ABD) and femoral (FEM...

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Autores principales: Lempesis, Ioannis G., Hoebers, Nicole, Essers, Yvonne, Jocken, Johan W. E., Dineen, Rosemary, Blaak, Ellen E., Manolopoulos, Konstantinos N., Goossens, Gijs H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10338223/
https://www.ncbi.nlm.nih.gov/pubmed/37455922
http://dx.doi.org/10.3389/fendo.2023.1205799
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author Lempesis, Ioannis G.
Hoebers, Nicole
Essers, Yvonne
Jocken, Johan W. E.
Dineen, Rosemary
Blaak, Ellen E.
Manolopoulos, Konstantinos N.
Goossens, Gijs H.
author_facet Lempesis, Ioannis G.
Hoebers, Nicole
Essers, Yvonne
Jocken, Johan W. E.
Dineen, Rosemary
Blaak, Ellen E.
Manolopoulos, Konstantinos N.
Goossens, Gijs H.
author_sort Lempesis, Ioannis G.
collection PubMed
description INTRODUCTION: Upper and lower body fat accumulation poses an opposing obesity-related cardiometabolic disease risk. Depot-differences in subcutaneous adipose tissue (SAT) function may underlie these associations. We aimed to investigate the inflammatory signatures of abdominal (ABD) and femoral (FEM) SAT in postmenopausal women with normal weight or obesity. METHODS: We included 23 postmenopausal women with normal weight (n = 13) or obesity (n = 10). In vivo secretion of adipokines from ABD and FEM SAT was measured using the arterio-venous balance technique. Adipokine gene expression and adipocyte morphology were examined in ABD and FEM SAT. Furthermore, adipokine expression and secretion were investigated in vitro using differentiated human primary ABD and FEM subcutaneous adipocytes derived from the study participants. RESULTS: Plasma leptin and plasminogen activator inhibitor (PAI)-1 concentrations were higher, and ABD and FEM adipocytes were larger in women with obesity than normal weight. No differences in adipocyte size and blood flow were apparent between ABD and FEM SAT. We found significant release of leptin and monocyte chemoattractant protein (MCP)-1 from ABD and FEM SAT, with higher fractional release of MCP-1 from ABD than FEM SAT. Gene expression of leptin, PAI-1, and tumor necrosis factor-α was lower in ABD than FEM SAT and higher in women with obesity than normal weight. In ABD adipocytes, interleukin-6, PAI-1, and leptin gene expression were higher, while adiponectin and dipeptidyl-peptidase-4 gene expression were lower than in FEM adipocytes. Finally, ABD adipocytes secreted less MCP-1 compared to FEM adipocytes. DISCUSSION: These findings demonstrate that upper and lower body SAT and adipocytes are characterized by distinct inflammatory signatures in postmenopausal women, which seem independent of adipocyte size.
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spelling pubmed-103382232023-07-14 Distinct inflammatory signatures of upper and lower body adipose tissue and adipocytes in women with normal weight or obesity Lempesis, Ioannis G. Hoebers, Nicole Essers, Yvonne Jocken, Johan W. E. Dineen, Rosemary Blaak, Ellen E. Manolopoulos, Konstantinos N. Goossens, Gijs H. Front Endocrinol (Lausanne) Endocrinology INTRODUCTION: Upper and lower body fat accumulation poses an opposing obesity-related cardiometabolic disease risk. Depot-differences in subcutaneous adipose tissue (SAT) function may underlie these associations. We aimed to investigate the inflammatory signatures of abdominal (ABD) and femoral (FEM) SAT in postmenopausal women with normal weight or obesity. METHODS: We included 23 postmenopausal women with normal weight (n = 13) or obesity (n = 10). In vivo secretion of adipokines from ABD and FEM SAT was measured using the arterio-venous balance technique. Adipokine gene expression and adipocyte morphology were examined in ABD and FEM SAT. Furthermore, adipokine expression and secretion were investigated in vitro using differentiated human primary ABD and FEM subcutaneous adipocytes derived from the study participants. RESULTS: Plasma leptin and plasminogen activator inhibitor (PAI)-1 concentrations were higher, and ABD and FEM adipocytes were larger in women with obesity than normal weight. No differences in adipocyte size and blood flow were apparent between ABD and FEM SAT. We found significant release of leptin and monocyte chemoattractant protein (MCP)-1 from ABD and FEM SAT, with higher fractional release of MCP-1 from ABD than FEM SAT. Gene expression of leptin, PAI-1, and tumor necrosis factor-α was lower in ABD than FEM SAT and higher in women with obesity than normal weight. In ABD adipocytes, interleukin-6, PAI-1, and leptin gene expression were higher, while adiponectin and dipeptidyl-peptidase-4 gene expression were lower than in FEM adipocytes. Finally, ABD adipocytes secreted less MCP-1 compared to FEM adipocytes. DISCUSSION: These findings demonstrate that upper and lower body SAT and adipocytes are characterized by distinct inflammatory signatures in postmenopausal women, which seem independent of adipocyte size. Frontiers Media S.A. 2023-06-26 /pmc/articles/PMC10338223/ /pubmed/37455922 http://dx.doi.org/10.3389/fendo.2023.1205799 Text en Copyright © 2023 Lempesis, Hoebers, Essers, Jocken, Dineen, Blaak, Manolopoulos and Goossens https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Endocrinology
Lempesis, Ioannis G.
Hoebers, Nicole
Essers, Yvonne
Jocken, Johan W. E.
Dineen, Rosemary
Blaak, Ellen E.
Manolopoulos, Konstantinos N.
Goossens, Gijs H.
Distinct inflammatory signatures of upper and lower body adipose tissue and adipocytes in women with normal weight or obesity
title Distinct inflammatory signatures of upper and lower body adipose tissue and adipocytes in women with normal weight or obesity
title_full Distinct inflammatory signatures of upper and lower body adipose tissue and adipocytes in women with normal weight or obesity
title_fullStr Distinct inflammatory signatures of upper and lower body adipose tissue and adipocytes in women with normal weight or obesity
title_full_unstemmed Distinct inflammatory signatures of upper and lower body adipose tissue and adipocytes in women with normal weight or obesity
title_short Distinct inflammatory signatures of upper and lower body adipose tissue and adipocytes in women with normal weight or obesity
title_sort distinct inflammatory signatures of upper and lower body adipose tissue and adipocytes in women with normal weight or obesity
topic Endocrinology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10338223/
https://www.ncbi.nlm.nih.gov/pubmed/37455922
http://dx.doi.org/10.3389/fendo.2023.1205799
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