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Oral and anal microbiome from HIV-exposed individuals: role of host-associated factors in taxa composition and metabolic pathways
Evidence indicates that the microbiome plays a significant role in HIV immunopathogenesis and associated complications. This study aimed to characterize the oral and anal microbiome of Men who have Sex with Men (MSM) and Transgender Women (TGW), with and without HIV. One hundred and thirty oral and...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10338440/ https://www.ncbi.nlm.nih.gov/pubmed/37438354 http://dx.doi.org/10.1038/s41522-023-00413-4 |
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author | Lacunza, Ezequiel Fink, Valeria Salas, María E. Canzoneri, Romina Naipauer, Julián Williams, Sion Coso, Omar Sued, Omar Cahn, Pedro Mesri, Enrique A. Abba, Martín C. |
author_facet | Lacunza, Ezequiel Fink, Valeria Salas, María E. Canzoneri, Romina Naipauer, Julián Williams, Sion Coso, Omar Sued, Omar Cahn, Pedro Mesri, Enrique A. Abba, Martín C. |
author_sort | Lacunza, Ezequiel |
collection | PubMed |
description | Evidence indicates that the microbiome plays a significant role in HIV immunopathogenesis and associated complications. This study aimed to characterize the oral and anal microbiome of Men who have Sex with Men (MSM) and Transgender Women (TGW), with and without HIV. One hundred and thirty oral and anal DNA-derived samples were obtained from 78 participants and subjected to shotgun metagenomics sequencing for further microbiome analysis. Significant differences in the microbiome composition were found among subjects associated with HIV infection, gender, sex behavior, CD4+ T-cell counts, antiretroviral therapy (ART), and the presence of HPV-associated precancerous anal lesions. Results confirm the occurrence of oncogenic viromes in this high HIV-risk population. The oral microbiome in HIV-associated cases exhibited an enrichment of bacteria associated with periodontal disease pathogenesis. Conversely, anal bacteria showed a significant decrease in HIV-infected subjects (Coprococcus comes, Finegoldia magna, Blautia obeum, Catenibacterium mitsuokai). TGW showed enrichment in species related to sexual transmission, which concurs that most recruited TGW are or have been sex workers. Prevotella bivia and Fusobacterium gonidiaformans were positively associated with anal precancerous lesions among HIV-infected subjects. The enrichment of Holdemanella biformis and C. comes was associated with detectable viral load and ART-untreated patients. Metabolic pathways were distinctly affected by predominant factors linked to sexual behavior or HIV pathogenesis. Gene family analysis identified bacterial gene signatures as potential prognostic and predictive biomarkers for HIV/AIDS-associated malignancies. Conclusions: Identified microbial features at accessible sites are potential biomarkers for predicting precancerous anal lesions and therapeutic targets for HIV immunopathogenesis. |
format | Online Article Text |
id | pubmed-10338440 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-103384402023-07-14 Oral and anal microbiome from HIV-exposed individuals: role of host-associated factors in taxa composition and metabolic pathways Lacunza, Ezequiel Fink, Valeria Salas, María E. Canzoneri, Romina Naipauer, Julián Williams, Sion Coso, Omar Sued, Omar Cahn, Pedro Mesri, Enrique A. Abba, Martín C. NPJ Biofilms Microbiomes Article Evidence indicates that the microbiome plays a significant role in HIV immunopathogenesis and associated complications. This study aimed to characterize the oral and anal microbiome of Men who have Sex with Men (MSM) and Transgender Women (TGW), with and without HIV. One hundred and thirty oral and anal DNA-derived samples were obtained from 78 participants and subjected to shotgun metagenomics sequencing for further microbiome analysis. Significant differences in the microbiome composition were found among subjects associated with HIV infection, gender, sex behavior, CD4+ T-cell counts, antiretroviral therapy (ART), and the presence of HPV-associated precancerous anal lesions. Results confirm the occurrence of oncogenic viromes in this high HIV-risk population. The oral microbiome in HIV-associated cases exhibited an enrichment of bacteria associated with periodontal disease pathogenesis. Conversely, anal bacteria showed a significant decrease in HIV-infected subjects (Coprococcus comes, Finegoldia magna, Blautia obeum, Catenibacterium mitsuokai). TGW showed enrichment in species related to sexual transmission, which concurs that most recruited TGW are or have been sex workers. Prevotella bivia and Fusobacterium gonidiaformans were positively associated with anal precancerous lesions among HIV-infected subjects. The enrichment of Holdemanella biformis and C. comes was associated with detectable viral load and ART-untreated patients. Metabolic pathways were distinctly affected by predominant factors linked to sexual behavior or HIV pathogenesis. Gene family analysis identified bacterial gene signatures as potential prognostic and predictive biomarkers for HIV/AIDS-associated malignancies. Conclusions: Identified microbial features at accessible sites are potential biomarkers for predicting precancerous anal lesions and therapeutic targets for HIV immunopathogenesis. Nature Publishing Group UK 2023-07-12 /pmc/articles/PMC10338440/ /pubmed/37438354 http://dx.doi.org/10.1038/s41522-023-00413-4 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Lacunza, Ezequiel Fink, Valeria Salas, María E. Canzoneri, Romina Naipauer, Julián Williams, Sion Coso, Omar Sued, Omar Cahn, Pedro Mesri, Enrique A. Abba, Martín C. Oral and anal microbiome from HIV-exposed individuals: role of host-associated factors in taxa composition and metabolic pathways |
title | Oral and anal microbiome from HIV-exposed individuals: role of host-associated factors in taxa composition and metabolic pathways |
title_full | Oral and anal microbiome from HIV-exposed individuals: role of host-associated factors in taxa composition and metabolic pathways |
title_fullStr | Oral and anal microbiome from HIV-exposed individuals: role of host-associated factors in taxa composition and metabolic pathways |
title_full_unstemmed | Oral and anal microbiome from HIV-exposed individuals: role of host-associated factors in taxa composition and metabolic pathways |
title_short | Oral and anal microbiome from HIV-exposed individuals: role of host-associated factors in taxa composition and metabolic pathways |
title_sort | oral and anal microbiome from hiv-exposed individuals: role of host-associated factors in taxa composition and metabolic pathways |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10338440/ https://www.ncbi.nlm.nih.gov/pubmed/37438354 http://dx.doi.org/10.1038/s41522-023-00413-4 |
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