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Controlling genetic heterogeneity in gene-edited hematopoietic stem cells by single-cell expansion

Gene editing using engineered nucleases frequently produces unintended genetic lesions in hematopoietic stem cells (HSCs). Gene-edited HSC cultures thus contain heterogeneous populations, the majority of which either do not carry the desired edit or harbor unwanted mutations. In consequence, transpl...

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Detalles Bibliográficos
Autores principales:	Becker, Hans Jiro, Ishida, Reiko, Wilkinson, Adam C., Kimura, Takaharu, Lee, Michelle Sue Jann, Coban, Cevayir, Ota, Yasunori, Tanaka, Yosuke, Roskamp, Meike, Sano, Tsubasa, Tojo, Arinobu, Kent, David G., Yamazaki, Satoshi
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Cell Press 2023
Materias:	Resource
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10338855/ https://www.ncbi.nlm.nih.gov/pubmed/37385251 http://dx.doi.org/10.1016/j.stem.2023.06.002

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