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Comparative predictive power of serum vs plasma proteomic signatures in feto-maternal medicine

BACKGROUND: Blood proteins are frequently measured in serum or plasma, because they provide a wealth of information. Differences in the ex vivo processing of serum and plasma raise concerns that proteomic health and disease signatures derived from serum or plasma differ in content and quality. Howev...

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Autores principales: Espinosa, Camilo, Ali, Said Mohammed, Khan, Waqasuddin, Khanam, Rasheda, Pervin, Jesmin, Price, Joan T., Rahman, Sayedur, Hasan, Tarik, Ahmed, Salahuddin, Raqib, Rubhana, Rahman, Monjur, Aktar, Shaki, Nisar, Muhammad I., Khalid, Javairia, Dhingra, Usha, Dutta, Arup, Deb, Saikat, Stringer, Jeffrey S.A., Wong, Ronald J., Shaw, Gary M., Stevenson, David K., Darmstadt, Gary L., Gaudilliere, Brice, Baqui, Abdullah H., Jehan, Fyezah, Rahman, Anisur, Sazawal, Sunil, Vwalika, Bellington, Aghaeepour, Nima, Angst, Martin S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10339042/
https://www.ncbi.nlm.nih.gov/pubmed/37456144
http://dx.doi.org/10.1016/j.xagr.2023.100244
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author Espinosa, Camilo
Ali, Said Mohammed
Khan, Waqasuddin
Khanam, Rasheda
Pervin, Jesmin
Price, Joan T.
Rahman, Sayedur
Hasan, Tarik
Ahmed, Salahuddin
Raqib, Rubhana
Rahman, Monjur
Aktar, Shaki
Nisar, Muhammad I.
Khalid, Javairia
Dhingra, Usha
Dutta, Arup
Deb, Saikat
Stringer, Jeffrey S.A.
Wong, Ronald J.
Shaw, Gary M.
Stevenson, David K.
Darmstadt, Gary L.
Gaudilliere, Brice
Baqui, Abdullah H.
Jehan, Fyezah
Rahman, Anisur
Sazawal, Sunil
Vwalika, Bellington
Aghaeepour, Nima
Angst, Martin S.
author_facet Espinosa, Camilo
Ali, Said Mohammed
Khan, Waqasuddin
Khanam, Rasheda
Pervin, Jesmin
Price, Joan T.
Rahman, Sayedur
Hasan, Tarik
Ahmed, Salahuddin
Raqib, Rubhana
Rahman, Monjur
Aktar, Shaki
Nisar, Muhammad I.
Khalid, Javairia
Dhingra, Usha
Dutta, Arup
Deb, Saikat
Stringer, Jeffrey S.A.
Wong, Ronald J.
Shaw, Gary M.
Stevenson, David K.
Darmstadt, Gary L.
Gaudilliere, Brice
Baqui, Abdullah H.
Jehan, Fyezah
Rahman, Anisur
Sazawal, Sunil
Vwalika, Bellington
Aghaeepour, Nima
Angst, Martin S.
author_sort Espinosa, Camilo
collection PubMed
description BACKGROUND: Blood proteins are frequently measured in serum or plasma, because they provide a wealth of information. Differences in the ex vivo processing of serum and plasma raise concerns that proteomic health and disease signatures derived from serum or plasma differ in content and quality. However, little is known about their respective power to predict feto-maternal health outcomes. Predictive power is a sentinel characteristic to determine the clinical use of biosignatures. OBJECTIVE: This study aimed to compare the power of serum and plasma proteomic signatures to predict a physiological pregnancy outcome. STUDY DESIGN: Paired serum and plasma samples from 73 women were obtained from biorepositories of a multinational prospective cohort study on pregnancy outcomes. Gestational age at the time of sampling was the predicted outcome, because the proteomic signatures have been validated for such a prediction. Multivariate and cross-validated models were independently derived for serum and plasma proteins. RESULTS: A total of 1116 proteins were measured in 88 paired samples from 73 women with a highly multiplexed platform using proximity extension technology (Olink Proteomics Inc, Watertown, MA). The plasma proteomic signature showed a higher predictive power (R=0.64; confidence interval, 0.42–0.79; P=3.5×10(-6)) than the serum signature (R=0.45; confidence interval, 0.18–0.66; P=2.2×10(-3)). The serum signature was validated in plasma with a similar predictive power (R=0.58; confidence interval, 0.34–0.75; P=4.8×10(-5)), whereas the plasma signature was validated in serum with reduced predictive power (R=0.53; confidence interval, 0.27–0.72; P=2.6×10(-4)). Signature proteins largely overlapped in the serum and plasma, but the strength of association with gestational age was weaker for serum proteins. CONCLUSION: Findings suggest that serum proteomics are less informative than plasma proteomics. They are compatible with the view that the partial ex-vivo degradation and modification of serum proteins during sample processing are an underlying reason. The rationale for collecting and analyzing serum and plasma samples should be carefully considered when deriving proteomic biosignatures to ascertain that specimens of the highest scientific and clinical yield are processed. Findings suggest that plasma is the preferred matrix.
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spelling pubmed-103390422023-07-14 Comparative predictive power of serum vs plasma proteomic signatures in feto-maternal medicine Espinosa, Camilo Ali, Said Mohammed Khan, Waqasuddin Khanam, Rasheda Pervin, Jesmin Price, Joan T. Rahman, Sayedur Hasan, Tarik Ahmed, Salahuddin Raqib, Rubhana Rahman, Monjur Aktar, Shaki Nisar, Muhammad I. Khalid, Javairia Dhingra, Usha Dutta, Arup Deb, Saikat Stringer, Jeffrey S.A. Wong, Ronald J. Shaw, Gary M. Stevenson, David K. Darmstadt, Gary L. Gaudilliere, Brice Baqui, Abdullah H. Jehan, Fyezah Rahman, Anisur Sazawal, Sunil Vwalika, Bellington Aghaeepour, Nima Angst, Martin S. AJOG Glob Rep Original Research BACKGROUND: Blood proteins are frequently measured in serum or plasma, because they provide a wealth of information. Differences in the ex vivo processing of serum and plasma raise concerns that proteomic health and disease signatures derived from serum or plasma differ in content and quality. However, little is known about their respective power to predict feto-maternal health outcomes. Predictive power is a sentinel characteristic to determine the clinical use of biosignatures. OBJECTIVE: This study aimed to compare the power of serum and plasma proteomic signatures to predict a physiological pregnancy outcome. STUDY DESIGN: Paired serum and plasma samples from 73 women were obtained from biorepositories of a multinational prospective cohort study on pregnancy outcomes. Gestational age at the time of sampling was the predicted outcome, because the proteomic signatures have been validated for such a prediction. Multivariate and cross-validated models were independently derived for serum and plasma proteins. RESULTS: A total of 1116 proteins were measured in 88 paired samples from 73 women with a highly multiplexed platform using proximity extension technology (Olink Proteomics Inc, Watertown, MA). The plasma proteomic signature showed a higher predictive power (R=0.64; confidence interval, 0.42–0.79; P=3.5×10(-6)) than the serum signature (R=0.45; confidence interval, 0.18–0.66; P=2.2×10(-3)). The serum signature was validated in plasma with a similar predictive power (R=0.58; confidence interval, 0.34–0.75; P=4.8×10(-5)), whereas the plasma signature was validated in serum with reduced predictive power (R=0.53; confidence interval, 0.27–0.72; P=2.6×10(-4)). Signature proteins largely overlapped in the serum and plasma, but the strength of association with gestational age was weaker for serum proteins. CONCLUSION: Findings suggest that serum proteomics are less informative than plasma proteomics. They are compatible with the view that the partial ex-vivo degradation and modification of serum proteins during sample processing are an underlying reason. The rationale for collecting and analyzing serum and plasma samples should be carefully considered when deriving proteomic biosignatures to ascertain that specimens of the highest scientific and clinical yield are processed. Findings suggest that plasma is the preferred matrix. Elsevier 2023-06-12 /pmc/articles/PMC10339042/ /pubmed/37456144 http://dx.doi.org/10.1016/j.xagr.2023.100244 Text en © 2023 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Original Research
Espinosa, Camilo
Ali, Said Mohammed
Khan, Waqasuddin
Khanam, Rasheda
Pervin, Jesmin
Price, Joan T.
Rahman, Sayedur
Hasan, Tarik
Ahmed, Salahuddin
Raqib, Rubhana
Rahman, Monjur
Aktar, Shaki
Nisar, Muhammad I.
Khalid, Javairia
Dhingra, Usha
Dutta, Arup
Deb, Saikat
Stringer, Jeffrey S.A.
Wong, Ronald J.
Shaw, Gary M.
Stevenson, David K.
Darmstadt, Gary L.
Gaudilliere, Brice
Baqui, Abdullah H.
Jehan, Fyezah
Rahman, Anisur
Sazawal, Sunil
Vwalika, Bellington
Aghaeepour, Nima
Angst, Martin S.
Comparative predictive power of serum vs plasma proteomic signatures in feto-maternal medicine
title Comparative predictive power of serum vs plasma proteomic signatures in feto-maternal medicine
title_full Comparative predictive power of serum vs plasma proteomic signatures in feto-maternal medicine
title_fullStr Comparative predictive power of serum vs plasma proteomic signatures in feto-maternal medicine
title_full_unstemmed Comparative predictive power of serum vs plasma proteomic signatures in feto-maternal medicine
title_short Comparative predictive power of serum vs plasma proteomic signatures in feto-maternal medicine
title_sort comparative predictive power of serum vs plasma proteomic signatures in feto-maternal medicine
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10339042/
https://www.ncbi.nlm.nih.gov/pubmed/37456144
http://dx.doi.org/10.1016/j.xagr.2023.100244
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