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Fabrication of functional ameloblasts from hiPSCs for dental application
Tooth formation relies on two types of dental cell populations, namely, the dental epithelium and dental mesenchyme, and the interactions between these cell populations are important during tooth development. Although human-induced pluripotent stem cells (hiPSCs) can differentiate into dental epithe...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10339106/ https://www.ncbi.nlm.nih.gov/pubmed/37457296 http://dx.doi.org/10.3389/fcell.2023.1164811 |
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author | Kim, Ka-Hwa Kim, Eun-Jung Kim, Hyun-Yi Li, Shujin Jung, Han-Sung |
author_facet | Kim, Ka-Hwa Kim, Eun-Jung Kim, Hyun-Yi Li, Shujin Jung, Han-Sung |
author_sort | Kim, Ka-Hwa |
collection | PubMed |
description | Tooth formation relies on two types of dental cell populations, namely, the dental epithelium and dental mesenchyme, and the interactions between these cell populations are important during tooth development. Although human-induced pluripotent stem cells (hiPSCs) can differentiate into dental epithelial and mesenchymal cells, organoid research on tooth development has not been established yet. This study focused on the hiPSC-derived human ameloblast organoid (hAO) using a three-dimensional (3D) culture system. hAOs had similar properties to ameloblasts, forming enamel in response to calcium and mineralization by interaction with the dental mesenchyme. hAOs simultaneously had osteogenic and odontogenic differentiation potential. Furthermore, hAOs demonstrated tooth regenerative potential upon interaction with the mouse dental mesenchyme. Our findings provide new insights into a suitable hiPSC-derived dental source and demonstrate that hAOs can be beneficial not only for tooth regeneration but also for the study of various dental diseases for which treatment has not been developed yet. |
format | Online Article Text |
id | pubmed-10339106 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-103391062023-07-14 Fabrication of functional ameloblasts from hiPSCs for dental application Kim, Ka-Hwa Kim, Eun-Jung Kim, Hyun-Yi Li, Shujin Jung, Han-Sung Front Cell Dev Biol Cell and Developmental Biology Tooth formation relies on two types of dental cell populations, namely, the dental epithelium and dental mesenchyme, and the interactions between these cell populations are important during tooth development. Although human-induced pluripotent stem cells (hiPSCs) can differentiate into dental epithelial and mesenchymal cells, organoid research on tooth development has not been established yet. This study focused on the hiPSC-derived human ameloblast organoid (hAO) using a three-dimensional (3D) culture system. hAOs had similar properties to ameloblasts, forming enamel in response to calcium and mineralization by interaction with the dental mesenchyme. hAOs simultaneously had osteogenic and odontogenic differentiation potential. Furthermore, hAOs demonstrated tooth regenerative potential upon interaction with the mouse dental mesenchyme. Our findings provide new insights into a suitable hiPSC-derived dental source and demonstrate that hAOs can be beneficial not only for tooth regeneration but also for the study of various dental diseases for which treatment has not been developed yet. Frontiers Media S.A. 2023-06-29 /pmc/articles/PMC10339106/ /pubmed/37457296 http://dx.doi.org/10.3389/fcell.2023.1164811 Text en Copyright © 2023 Kim, Kim, Kim, Li and Jung. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cell and Developmental Biology Kim, Ka-Hwa Kim, Eun-Jung Kim, Hyun-Yi Li, Shujin Jung, Han-Sung Fabrication of functional ameloblasts from hiPSCs for dental application |
title | Fabrication of functional ameloblasts from hiPSCs for dental application |
title_full | Fabrication of functional ameloblasts from hiPSCs for dental application |
title_fullStr | Fabrication of functional ameloblasts from hiPSCs for dental application |
title_full_unstemmed | Fabrication of functional ameloblasts from hiPSCs for dental application |
title_short | Fabrication of functional ameloblasts from hiPSCs for dental application |
title_sort | fabrication of functional ameloblasts from hipscs for dental application |
topic | Cell and Developmental Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10339106/ https://www.ncbi.nlm.nih.gov/pubmed/37457296 http://dx.doi.org/10.3389/fcell.2023.1164811 |
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