Protocol for generating monoclonal CRISPR-Cas9-mediated knockout cell lines using RNPs and lipofection in HNSCC cells

CRISPR-Cas9 is a powerful technology for accurate and optimizable genome editing. Here, we present a protocol for generating monoclonal knockout (KO) cell lines using CRISPR-Cas9, ribonucleoprotein complexes (RNPs), and lipofection in adherent HNSCC cells from start to finish. We describe steps for...

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Detalles Bibliográficos
Autores principales: Geyer, Fabian, Geyer, Maximilian, Klapproth, Sarah, Wolff, Klaus-Dietrich, Nieberler, Markus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10339245/
https://www.ncbi.nlm.nih.gov/pubmed/37421616
http://dx.doi.org/10.1016/j.xpro.2023.102366

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