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Protocol for diagnosing Erwinia amylovora infection using a fluorescent probe

Current fire blight diagnosis techniques are DNA based and require specialized equipment and expertise, or they are less sensitive. Here, we present a protocol for diagnosing fire blight using the fluorescent probe, B-1. We describe steps for Erwinia amylovora culture, implementing a fire blight-inf...

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Detalles Bibliográficos
Autores principales: Jin, Ji Hye, Kim, Dokyoung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10339246/
https://www.ncbi.nlm.nih.gov/pubmed/37421612
http://dx.doi.org/10.1016/j.xpro.2023.102412
Descripción
Sumario:Current fire blight diagnosis techniques are DNA based and require specialized equipment and expertise, or they are less sensitive. Here, we present a protocol for diagnosing fire blight using the fluorescent probe, B-1. We describe steps for Erwinia amylovora culture, implementing a fire blight-infected model, and E. amylovora visualization. This protocol allows for detection of fire blight bacteria of up to 10(2) CFU/mL on plants or objects in just 10 s with a simple application including spraying and swabbing. For complete details on the use and execution of this protocol, please refer to Jung et al.(1)