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Choice of Protein, Not Its Amyloid-Fold, Determines the Success of Amyloid-Based Scaffolds for Cartilage Tissue Regeneration
[Image: see text] The formation of fibrocartilage during articular cartilage regeneration remains a clinical problem affecting adequate restoration of articular cartilage in joints. To stimulate chondrocytes to form articular cartilage, we investigated the use of amyloid fibril-based scaffolds. The...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10339334/ https://www.ncbi.nlm.nih.gov/pubmed/37457450 http://dx.doi.org/10.1021/acsomega.3c00151 |
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author | van Dalen, Maurice C.E. Karperien, Marcel Claessens, Mireille M.A.E. Post, Janine N. |
author_facet | van Dalen, Maurice C.E. Karperien, Marcel Claessens, Mireille M.A.E. Post, Janine N. |
author_sort | van Dalen, Maurice C.E. |
collection | PubMed |
description | [Image: see text] The formation of fibrocartilage during articular cartilage regeneration remains a clinical problem affecting adequate restoration of articular cartilage in joints. To stimulate chondrocytes to form articular cartilage, we investigated the use of amyloid fibril-based scaffolds. The proteins α-synuclein, β-lactoglobulin, and lysozyme were induced to self-assemble into amyloid fibrils and, during dialysis, formed micrometer scale amyloid networks that resemble the cartilage extracellular matrix. Our results show that lysozyme amyloid micronetworks supported chondrocyte viability and extracellular matrix deposition, while α-synuclein and β-lactoglobulin maintained cell viability. With this study, we not only confirm the possible use of amyloid materials for tissue regeneration but also demonstrate that the choice of protein, rather than its amyloid-fold per se, affects the cellular response and tissue formation. |
format | Online Article Text |
id | pubmed-10339334 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-103393342023-07-14 Choice of Protein, Not Its Amyloid-Fold, Determines the Success of Amyloid-Based Scaffolds for Cartilage Tissue Regeneration van Dalen, Maurice C.E. Karperien, Marcel Claessens, Mireille M.A.E. Post, Janine N. ACS Omega [Image: see text] The formation of fibrocartilage during articular cartilage regeneration remains a clinical problem affecting adequate restoration of articular cartilage in joints. To stimulate chondrocytes to form articular cartilage, we investigated the use of amyloid fibril-based scaffolds. The proteins α-synuclein, β-lactoglobulin, and lysozyme were induced to self-assemble into amyloid fibrils and, during dialysis, formed micrometer scale amyloid networks that resemble the cartilage extracellular matrix. Our results show that lysozyme amyloid micronetworks supported chondrocyte viability and extracellular matrix deposition, while α-synuclein and β-lactoglobulin maintained cell viability. With this study, we not only confirm the possible use of amyloid materials for tissue regeneration but also demonstrate that the choice of protein, rather than its amyloid-fold per se, affects the cellular response and tissue formation. American Chemical Society 2023-06-14 /pmc/articles/PMC10339334/ /pubmed/37457450 http://dx.doi.org/10.1021/acsomega.3c00151 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | van Dalen, Maurice C.E. Karperien, Marcel Claessens, Mireille M.A.E. Post, Janine N. Choice of Protein, Not Its Amyloid-Fold, Determines the Success of Amyloid-Based Scaffolds for Cartilage Tissue Regeneration |
title | Choice of Protein,
Not Its Amyloid-Fold, Determines
the Success of Amyloid-Based Scaffolds for Cartilage Tissue Regeneration |
title_full | Choice of Protein,
Not Its Amyloid-Fold, Determines
the Success of Amyloid-Based Scaffolds for Cartilage Tissue Regeneration |
title_fullStr | Choice of Protein,
Not Its Amyloid-Fold, Determines
the Success of Amyloid-Based Scaffolds for Cartilage Tissue Regeneration |
title_full_unstemmed | Choice of Protein,
Not Its Amyloid-Fold, Determines
the Success of Amyloid-Based Scaffolds for Cartilage Tissue Regeneration |
title_short | Choice of Protein,
Not Its Amyloid-Fold, Determines
the Success of Amyloid-Based Scaffolds for Cartilage Tissue Regeneration |
title_sort | choice of protein,
not its amyloid-fold, determines
the success of amyloid-based scaffolds for cartilage tissue regeneration |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10339334/ https://www.ncbi.nlm.nih.gov/pubmed/37457450 http://dx.doi.org/10.1021/acsomega.3c00151 |
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