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Raman, WAXS, and Solid-State NMR Characterizations of Regenerated Silk Fibroin Using Lanthanide Ions as Chaotropic Agents

[Image: see text] Bombyx mori silk fibroin (SF) has been reported as a convenient natural material for regenerative medicine, optoelectronics, and many other technological applications. SF owes its unique features to the hierarchical organization of the fibers. Many efforts have been made to set up...

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Autores principales: Rizzo, Giorgio, Petrelli, Valentina, Sibillano, Teresa, De Caro, Liberato, Giangregorio, Maria Michela, Lo Presti, Marco, Omenetto, Fiorenzo G., Giannini, Cinzia, Mastrorilli, Piero, Farinola, Gianluca M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10339335/
https://www.ncbi.nlm.nih.gov/pubmed/37457447
http://dx.doi.org/10.1021/acsomega.2c07149
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author Rizzo, Giorgio
Petrelli, Valentina
Sibillano, Teresa
De Caro, Liberato
Giangregorio, Maria Michela
Lo Presti, Marco
Omenetto, Fiorenzo G.
Giannini, Cinzia
Mastrorilli, Piero
Farinola, Gianluca M.
author_facet Rizzo, Giorgio
Petrelli, Valentina
Sibillano, Teresa
De Caro, Liberato
Giangregorio, Maria Michela
Lo Presti, Marco
Omenetto, Fiorenzo G.
Giannini, Cinzia
Mastrorilli, Piero
Farinola, Gianluca M.
author_sort Rizzo, Giorgio
collection PubMed
description [Image: see text] Bombyx mori silk fibroin (SF) has been reported as a convenient natural material for regenerative medicine, optoelectronics, and many other technological applications. SF owes its unique features to the hierarchical organization of the fibers. Many efforts have been made to set up protocols for dissolution since many applications of SF are based on regenerated solutions and fibers, but chaotropic conditions required to disassemble the packing of the polymer afford solutions with poor crystalline behavior. Our previous research has disclosed a dissolution and regeneration process of highly crystalline fibers involving lanthanide ions as chaotropic agents, demonstrating that each lanthanide has its own unique interaction with SF. Herein, we report elucidation of the structure of Ln–SF fibers by the combined use of Raman spectroscopy, wide-angle X-ray scattering (WAXS), and solid-state NMR techniques. Raman spectra confirmed the coordination of metal ions to SF, WAXS results highlighted the crystalline content of fibers, and solid-state NMR enabled the assessment of different ratios of secondary structures in the protein.
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spelling pubmed-103393352023-07-14 Raman, WAXS, and Solid-State NMR Characterizations of Regenerated Silk Fibroin Using Lanthanide Ions as Chaotropic Agents Rizzo, Giorgio Petrelli, Valentina Sibillano, Teresa De Caro, Liberato Giangregorio, Maria Michela Lo Presti, Marco Omenetto, Fiorenzo G. Giannini, Cinzia Mastrorilli, Piero Farinola, Gianluca M. ACS Omega [Image: see text] Bombyx mori silk fibroin (SF) has been reported as a convenient natural material for regenerative medicine, optoelectronics, and many other technological applications. SF owes its unique features to the hierarchical organization of the fibers. Many efforts have been made to set up protocols for dissolution since many applications of SF are based on regenerated solutions and fibers, but chaotropic conditions required to disassemble the packing of the polymer afford solutions with poor crystalline behavior. Our previous research has disclosed a dissolution and regeneration process of highly crystalline fibers involving lanthanide ions as chaotropic agents, demonstrating that each lanthanide has its own unique interaction with SF. Herein, we report elucidation of the structure of Ln–SF fibers by the combined use of Raman spectroscopy, wide-angle X-ray scattering (WAXS), and solid-state NMR techniques. Raman spectra confirmed the coordination of metal ions to SF, WAXS results highlighted the crystalline content of fibers, and solid-state NMR enabled the assessment of different ratios of secondary structures in the protein. American Chemical Society 2023-06-28 /pmc/articles/PMC10339335/ /pubmed/37457447 http://dx.doi.org/10.1021/acsomega.2c07149 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Rizzo, Giorgio
Petrelli, Valentina
Sibillano, Teresa
De Caro, Liberato
Giangregorio, Maria Michela
Lo Presti, Marco
Omenetto, Fiorenzo G.
Giannini, Cinzia
Mastrorilli, Piero
Farinola, Gianluca M.
Raman, WAXS, and Solid-State NMR Characterizations of Regenerated Silk Fibroin Using Lanthanide Ions as Chaotropic Agents
title Raman, WAXS, and Solid-State NMR Characterizations of Regenerated Silk Fibroin Using Lanthanide Ions as Chaotropic Agents
title_full Raman, WAXS, and Solid-State NMR Characterizations of Regenerated Silk Fibroin Using Lanthanide Ions as Chaotropic Agents
title_fullStr Raman, WAXS, and Solid-State NMR Characterizations of Regenerated Silk Fibroin Using Lanthanide Ions as Chaotropic Agents
title_full_unstemmed Raman, WAXS, and Solid-State NMR Characterizations of Regenerated Silk Fibroin Using Lanthanide Ions as Chaotropic Agents
title_short Raman, WAXS, and Solid-State NMR Characterizations of Regenerated Silk Fibroin Using Lanthanide Ions as Chaotropic Agents
title_sort raman, waxs, and solid-state nmr characterizations of regenerated silk fibroin using lanthanide ions as chaotropic agents
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10339335/
https://www.ncbi.nlm.nih.gov/pubmed/37457447
http://dx.doi.org/10.1021/acsomega.2c07149
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