Cargando…

Dual-color Colocalization in Single-molecule Localization Microscopy to Determine the Oligomeric State of Proteins in the Plasma Membrane

Determining the oligomeric state of membrane proteins is critical for understanding their function. However, traditional ex situ methods like clear native gel electrophoresis can disrupt protein subunit interactions during sample preparation. In situ methods such as stepwise photobleaching have limi...

Descripción completa

Detalles Bibliográficos
Autores principales:	Tan, Hua Leonhard, Bungert-Plümke, Stefanie, Kortzak, Daniel, Fahlke, Christoph, Stölting, Gabriel
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Bio-Protocol 2023
Materias:	Methods Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10339340/ https://www.ncbi.nlm.nih.gov/pubmed/37456335 http://dx.doi.org/10.21769/BioProtoc.4749

Ejemplares similares

Determination of oligomeric states of proteins via dual-color colocalization with single molecule localization microscopy
por: Tan, Hua Leonhard, et al.
Publicado: (2022)

Reduced Membrane Insertion of CLC-K by V33L Barttin Results in Loss of Hearing, but Leaves Kidney Function Intact
por: Tan, Hua, et al.
Publicado: (2017)

The method for integrating dual-color fluorescence colocalization and single molecule photobleaching technology on the theophylline sensing platform
por: Zhang, Dong, et al.
Publicado: (2020)

Reconstitution and NMR Characterization of the Ion-Channel Accessory Subunit Barttin in Detergents and Lipid-Bilayer Nanodiscs
por: Viennet, Thibault, et al.
Publicado: (2019)

Functional Characterization of CLCN4 Variants Associated With X-Linked Intellectual Disability and Epilepsy
por: Guzman, Raul E., et al.
Publicado: (2022)

Dual spatially resolved transcriptomics for human host–pathogen colocalization studies in FFPE tissue sections
por: Sounart, Hailey, et al.
Publicado: (2023)

Fluorescence colocalization microscopy analysis can be improved by combining object‐recognition with pixel‐intensity‐correlation
por: Moser, Bernhard, et al.
Publicado: (2016)

Analysis of conditional colocalization relationships and hierarchies in three-color microscopy images
por: Vega-Lugo, Jesus, et al.
Publicado: (2022)

Scalable and model-free detection of spatial patterns and colocalization
por: Liu, Qi, et al.
Publicado: (2022)

Site-specific dual-color labeling of long RNAs for single-molecule spectroscopy
por: Zhao, Meng, et al.
Publicado: (2018)

ClC-3 regulates the excitability of nociceptive neurons and is involved in inflammatory processes within the spinal sensory pathway
por: Sierra-Marquez, Juan, et al.
Publicado: (2022)

Corrigendum: ClC-3 regulates the excitability of nociceptive neurons and is involved in inflammatory processes within the spinal sensory pathway
por: Sierra-Marquez, Juan, et al.
Publicado: (2023)

CLC channel function and dysfunction in health and disease
por: Stölting, Gabriel, et al.
Publicado: (2014)

A method for colocalizing lineage tracing reporter and RNAscope signals on skeletal tissue section
por: Yang, Huiliang, et al.
Publicado: (2021)

A tessellation-based colocalization analysis approach for single-molecule localization microscopy
por: Levet, Florian, et al.
Publicado: (2019)

Hessian single-molecule localization microscopy using sCMOS camera
por: Xue, Fudong, et al.
Publicado: (2018)

Zinc modulation of proton currents in a new voltage‐gated proton channel suggests a mechanism of inhibition
por: Chaves, Gustavo, et al.
Publicado: (2020)

CoLoC-seq probes the global topology of organelle transcriptomes
por: Jeandard, Damien, et al.
Publicado: (2022)

A density-based enrichment measure for assessing colocalization in single-molecule localization microscopy data
por: Ejdrup, Aske L., et al.
Publicado: (2022)

Immunofluorescence microscopy of SNAP23 in human skeletal muscle reveals colocalization with plasma membrane, lipid droplets, and mitochondria
por: Strauss, Juliette A., et al.
Publicado: (2016)

Molecular Basis of Coupled Transport and Anion Conduction in Excitatory Amino Acid Transporters
por: Alleva, Claudia, et al.
Publicado: (2021)

Apo state pore opening as functional basis of increased EAAT anion channel activity in episodic ataxia 6
por: Suslova, Mariia, et al.
Publicado: (2023)

Colocalized, bidirectional optogenetic modulations in freely behaving mice with a wireless dual-color optoelectronic probe
por: Li, Lizhu, et al.
Publicado: (2022)

Two-color STED microscopy reveals different degrees of colocalization between hexokinase-I and the three human VDAC isoforms
por: Neumann, Daniel, et al.
Publicado: (2010)

Purification Analysis, Intracellular Tracking, and Colocalization of Extracellular Vesicles Using Atomic Force and 3D Single-Molecule Localization Microscopy
por: Puthukodan, Sujitha, et al.
Publicado: (2023)

Clus-DoC: a combined cluster detection and colocalization analysis for single-molecule localization microscopy data
por: Pageon, Sophie V., et al.
Publicado: (2016)

Virtual reality assisted microscopy data visualization and colocalization analysis
por: Theart, Rensu P., et al.
Publicado: (2017)

Methyl-SNP-seq reveals dual readouts of methylome and variome at molecule resolution while enabling target enrichment
por: Yan, Bo, et al.
Publicado: (2022)

Visualization of uracils created by APOBEC3A using UdgX shows colocalization with RPA at stalled replication forks
por: Stewart, Jessica A, et al.
Publicado: (2020)

Impaired K(+) binding to glial glutamate transporter EAAT1 in migraine
por: Kovermann, Peter, et al.
Publicado: (2017)

Controlled Level of Contamination Coupled to Deep Sequencing (CoLoC-seq) Probes the Global Localisation Topology of Organelle Transcriptomes
por: Smirnova, Anna, et al.
Publicado: (2023)

Novel approach for accurate tissue-based protein colocalization and proximity microscopy
por: Lutz, Mirjam I., et al.
Publicado: (2017)

Chromatin dynamics through mouse preimplantation development revealed by single molecule localisation microscopy
por: Portela, Marta, et al.
Publicado: (2022)

A method for imaging single molecules at the plasma membrane of live cells within tissue slices
por: Mashanov, Gregory I., et al.
Publicado: (2020)

A workflow for sizing oligomeric biomolecules based on cryo single molecule localization microscopy
por: Schneider, Magdalena C., et al.
Publicado: (2021)

Dual-Color Live Imaging of Adult Muscle Stem Cells in the Embryonic Tissues of Drosophila melanogaster
por: Zmojdzian, Monika, et al.
Publicado: (2023)

Rapid Turnover of the Cardiac L-Type Ca(V)1.2 Channel by Endocytic Recycling Regulates Its Cell Surface Availability
por: Conrad, Rachel, et al.
Publicado: (2018)

Neuronal cell adhesion molecules and cytotactin are colocalized at the node of Ranvier
Publicado: (1986)

Improved immunoassay sensitivity and specificity using single-molecule colocalization
por: Hariri, Amani A., et al.
Publicado: (2022)

Quantification of DNA-associated proteins inside eukaryotic cells using single-molecule localization microscopy
por: Etheridge, Thomas J., et al.
Publicado: (2014)

Cannot write session to /tmp/vufind_sessions/sess_1dich29erlh8h84c9vjthnrib8