Biosynthesis of Silver Nanoparticles Functionalized with Histidine and Phenylalanine Amino Acids for Potential Antioxidant and Antibacterial Activities

[Image: see text] Due to biochemically active secondary metabolites that assist in the reduction, stabilization, and capping of nanoparticles, plant-mediated nanoparticle synthesis is becoming more and more popular. This is because it allows for ecologically friendly, feasible, sustainable, and cost-effective green synthesis techniques. This study describes the biosynthesis of silver nanoparticles (AgNPs) functionalized with histidine and phenylalanine using the Lippia abyssinica (locally called koseret) plant leaf extract. The functionalization with amino acids was meant to enhance the biological activities of the AgNPs. The synthesized nanoparticles were characterized using UV–Visible absorption (UV–Vis), powder X-ray diffraction (pXRD), scanning electron microscopy (SEM), energy-dispersive X-ray (EDX) spectroscopy, transmission electron microscopy (TEM), and Fourier transform infrared (FTIR) spectroscopy. The surface plasmonic resonance (SPR) peak at about 433 nm confirmed the biosynthesis of the AgNPs. FTIR spectra also revealed that the phytochemicals in the plant extract were responsible for the capping of the biogenically synthesized AgNPs. On the other hand, the TEM micrograph revealed that the morphology of AgNP-His had diameters ranging from 5 to 14 nm. The antibacterial activities of the synthesized nanoparticles against Gram-positive and Gram-negative bacteria showed a growth inhibition of 8.67 ± 1.25 and 11.00 ± 0.82 mm against Escherichia coli and Staphylococcus aureus, respectively, at a concentration of 62.5 μg/mL AgNP-His. Moreover, the nanoparticle has an antioxidant activity potential of 63.76 ± 1.25% at 250 μg/mL. The results showed that the green-synthesized AgNPs possess promising antioxidant and antibacterial activities with the potential for biological applications.