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Evaluation of surgical fixation methods for the implantation of melt electrowriting-reinforced hyaluronic acid hydrogel composites in porcine cartilage defects

The surgical repair of articular cartilage remains an ongoing challenge in orthopedics. Tissue engineering is a promising approach to treat cartilage defects; however, scaffolds must (i) possess the requisite material properties to support neocartilage formation, (ii) exhibit sufficient mechanical i...

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Autores principales: Galarraga, Jonathan H., Zlotnick, Hannah M., Locke, Ryan C., Gupta, Sachin, Fogarty, Natalie L., Masada, Kendall M., Stoeckl, Brendan D., Laforest, Lorielle, Castilho, Miguel, Malda, Jos, Levato, Riccardo, Carey, James L., Mauck, Robert L., Burdick, Jason A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Whioce Publishing Pte. Ltd. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10339416/
https://www.ncbi.nlm.nih.gov/pubmed/37457945
http://dx.doi.org/10.18063/ijb.775
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author Galarraga, Jonathan H.
Zlotnick, Hannah M.
Locke, Ryan C.
Gupta, Sachin
Fogarty, Natalie L.
Masada, Kendall M.
Stoeckl, Brendan D.
Laforest, Lorielle
Castilho, Miguel
Malda, Jos
Levato, Riccardo
Carey, James L.
Mauck, Robert L.
Burdick, Jason A.
author_facet Galarraga, Jonathan H.
Zlotnick, Hannah M.
Locke, Ryan C.
Gupta, Sachin
Fogarty, Natalie L.
Masada, Kendall M.
Stoeckl, Brendan D.
Laforest, Lorielle
Castilho, Miguel
Malda, Jos
Levato, Riccardo
Carey, James L.
Mauck, Robert L.
Burdick, Jason A.
author_sort Galarraga, Jonathan H.
collection PubMed
description The surgical repair of articular cartilage remains an ongoing challenge in orthopedics. Tissue engineering is a promising approach to treat cartilage defects; however, scaffolds must (i) possess the requisite material properties to support neocartilage formation, (ii) exhibit sufficient mechanical integrity for handling during implantation, and (iii) be reliably fixed within cartilage defects during surgery. In this study, we demonstrate the reinforcement of soft norbornene-modified hyaluronic acid (NorHA) hydrogels via the melt electrowriting (MEW) of polycaprolactone to fabricate composite scaffolds that support encapsulated porcine mesenchymal stromal cell (pMSC, three donors) chondrogenesis and cartilage formation and exhibit mechanical properties suitable for handling during implantation. Thereafter, acellular MEW-NorHA composites or MEW-NorHA composites with encapsulated pMSCs and precultured for 28 days were implanted in full-thickness cartilage defects in porcine knees using either bioresorbable pins or fibrin glue to assess surgical fixation methods. Fixation of composites with either biodegradable pins or fibrin glue ensured implant retention in most cases (80%); however, defects treated with pinned composites exhibited more subchondral bone remodeling and inferior cartilage repair, as evidenced by micro-computed tomography (micro-CT) and safranin O/fast green staining, respectively, when compared to defects treated with glued composites. Interestingly, no differences in repair tissue were observed between acellular and cellularized implants. Additional work is required to assess the full potential of these scaffolds for cartilage repair. However, these results suggest that future approaches for cartilage repair with MEW-reinforced hydrogels should be carefully evaluated with regard to their fixation approach for construct retention and surrounding cartilage tissue damage.
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spelling pubmed-103394162023-07-14 Evaluation of surgical fixation methods for the implantation of melt electrowriting-reinforced hyaluronic acid hydrogel composites in porcine cartilage defects Galarraga, Jonathan H. Zlotnick, Hannah M. Locke, Ryan C. Gupta, Sachin Fogarty, Natalie L. Masada, Kendall M. Stoeckl, Brendan D. Laforest, Lorielle Castilho, Miguel Malda, Jos Levato, Riccardo Carey, James L. Mauck, Robert L. Burdick, Jason A. Int J Bioprint Research Article The surgical repair of articular cartilage remains an ongoing challenge in orthopedics. Tissue engineering is a promising approach to treat cartilage defects; however, scaffolds must (i) possess the requisite material properties to support neocartilage formation, (ii) exhibit sufficient mechanical integrity for handling during implantation, and (iii) be reliably fixed within cartilage defects during surgery. In this study, we demonstrate the reinforcement of soft norbornene-modified hyaluronic acid (NorHA) hydrogels via the melt electrowriting (MEW) of polycaprolactone to fabricate composite scaffolds that support encapsulated porcine mesenchymal stromal cell (pMSC, three donors) chondrogenesis and cartilage formation and exhibit mechanical properties suitable for handling during implantation. Thereafter, acellular MEW-NorHA composites or MEW-NorHA composites with encapsulated pMSCs and precultured for 28 days were implanted in full-thickness cartilage defects in porcine knees using either bioresorbable pins or fibrin glue to assess surgical fixation methods. Fixation of composites with either biodegradable pins or fibrin glue ensured implant retention in most cases (80%); however, defects treated with pinned composites exhibited more subchondral bone remodeling and inferior cartilage repair, as evidenced by micro-computed tomography (micro-CT) and safranin O/fast green staining, respectively, when compared to defects treated with glued composites. Interestingly, no differences in repair tissue were observed between acellular and cellularized implants. Additional work is required to assess the full potential of these scaffolds for cartilage repair. However, these results suggest that future approaches for cartilage repair with MEW-reinforced hydrogels should be carefully evaluated with regard to their fixation approach for construct retention and surrounding cartilage tissue damage. Whioce Publishing Pte. Ltd. 2023-06-14 /pmc/articles/PMC10339416/ /pubmed/37457945 http://dx.doi.org/10.18063/ijb.775 Text en Copyright:© 2023, Galarraga JH, Zlotnick HM, Locke RC, et al https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, permitting distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Galarraga, Jonathan H.
Zlotnick, Hannah M.
Locke, Ryan C.
Gupta, Sachin
Fogarty, Natalie L.
Masada, Kendall M.
Stoeckl, Brendan D.
Laforest, Lorielle
Castilho, Miguel
Malda, Jos
Levato, Riccardo
Carey, James L.
Mauck, Robert L.
Burdick, Jason A.
Evaluation of surgical fixation methods for the implantation of melt electrowriting-reinforced hyaluronic acid hydrogel composites in porcine cartilage defects
title Evaluation of surgical fixation methods for the implantation of melt electrowriting-reinforced hyaluronic acid hydrogel composites in porcine cartilage defects
title_full Evaluation of surgical fixation methods for the implantation of melt electrowriting-reinforced hyaluronic acid hydrogel composites in porcine cartilage defects
title_fullStr Evaluation of surgical fixation methods for the implantation of melt electrowriting-reinforced hyaluronic acid hydrogel composites in porcine cartilage defects
title_full_unstemmed Evaluation of surgical fixation methods for the implantation of melt electrowriting-reinforced hyaluronic acid hydrogel composites in porcine cartilage defects
title_short Evaluation of surgical fixation methods for the implantation of melt electrowriting-reinforced hyaluronic acid hydrogel composites in porcine cartilage defects
title_sort evaluation of surgical fixation methods for the implantation of melt electrowriting-reinforced hyaluronic acid hydrogel composites in porcine cartilage defects
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10339416/
https://www.ncbi.nlm.nih.gov/pubmed/37457945
http://dx.doi.org/10.18063/ijb.775
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