Collagen Biocomposites Derived from Fish Waste: Doped and Cross-Linked with Functionalized Fe(3)O(4) Nanoparticles and Their Comparative Studies with a Green Approach

[Image: see text] Collagen-based nanobiocomposites can reabsorb and are biodegradable. These properties are effectively controlled by the number of cross-links. This study demonstrates an effortless and proficient approach for the functionalization of Fe(3)O(4) NPs for cross-linking collagen obtained from biowaste, viz., fish scales of Lates Calcarifer, a marine origin. The size of Fe(3)O(4) NPs (10–40 nm) was confirmed using particle size analysis. The physico-chemical properties of the aminosilane-coated Fe(3)O(4) NPs cross-linked via succinylated collagen (FFCSC) were characterized using different analytical techniques and compared with succinylated collagen doped with Fe(3)O(4) NPs (FDSC). Thermogravimetric analysis indicates cross-linked product FFCSC to be more stable than the FDSC. Also, the antibacterial effect was more pronounced for FFCSC than for FDSC nanobiocomposites. FFCSC exhibited improved mechanical properties which are essential for materials used for wound dressing purposes. Moreover, the cell viability of fibroblasts (3T3-L1) and their morphology studied by SEM and fluorescence microscopy showed biocompatibility of both FDSC and FFCSC. Thus, the current investigation, involves a waste to wealth approach where the collagen-based nanobiocomposites present an easy way to recycle the biowaste to value-added products using simple and clean methods, which are suitable for use in biomedical and environmental applications.