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A Selection of 14 Tetrameric Microsatellite Markers for Genetic Investigations in Fallow Deer (Dama dama)

SIMPLE SUMMARY: Monitoring and maintaining genetic diversity is essential for the conservation of most species, including fallow deer. Various markers can be tested to estimate genetic diversity. For this purpose, microsatellites consisting of four-base-pair repetitive units are highly recommended d...

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Autores principales: Zorkóczy, Orsolya Krisztina, Turi, Orsolya, Wagenhoffer, Zsombor, Ózsvári, László, Lehotzky, Pál, Pádár, Zsolt, Zenke, Petra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10339914/
https://www.ncbi.nlm.nih.gov/pubmed/37443886
http://dx.doi.org/10.3390/ani13132083
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author Zorkóczy, Orsolya Krisztina
Turi, Orsolya
Wagenhoffer, Zsombor
Ózsvári, László
Lehotzky, Pál
Pádár, Zsolt
Zenke, Petra
author_facet Zorkóczy, Orsolya Krisztina
Turi, Orsolya
Wagenhoffer, Zsombor
Ózsvári, László
Lehotzky, Pál
Pádár, Zsolt
Zenke, Petra
author_sort Zorkóczy, Orsolya Krisztina
collection PubMed
description SIMPLE SUMMARY: Monitoring and maintaining genetic diversity is essential for the conservation of most species, including fallow deer. Various markers can be tested to estimate genetic diversity. For this purpose, microsatellites consisting of four-base-pair repetitive units are highly recommended due to their polymorphisms and comparable and reliable detection during analyses. In this study, nearly one hundred tetrameric microsatellites were collected from nine other deer species, which were tested on twenty individuals from five Hungarian fallow deer populations. As a result, 14 polymorphic markers were selected for the investigation panel, which fills a gap in the field of experts dealing with fallow deer genetics. ABSTRACT: The fallow deer (Dama dama) represents significant game management value globally, and human activities are significantly impacting the species. Besides the positive effects, these activities can threaten its existence, health, and value. The aim of the authors was to develop a tetranucleotide microsatellite panel that could be clearly interpreted and used for genetic testing of fallow deer. Such a panel did not exist until now and could be particularly useful in the field of conservation genetics and forensics. A total of 99 tetrameric microsatellites, originally designed for related deer species, were tested on 20 fallow deer individuals from five Hungarian sampling areas. Original and newly designed primers were used to amplify the microsatellite regions using previously published or optimized PCR protocols. The lengths and sequences of specific amplicons were detected using capillary electrophoresis, and the rate of polymorphism was determined. Altogether, 80 markers provided PCR products of adequate quality and quantity. Among them, 15 markers proved to be polymorphic (2–5 alleles/locus), and 14 tetrameric markers were selected for further analysis. Statistical calculations showed that the selected polymorphic microsatellites can potentially enable key individualization in many areas of wildlife and population genetics, thus protecting the species.
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spelling pubmed-103399142023-07-14 A Selection of 14 Tetrameric Microsatellite Markers for Genetic Investigations in Fallow Deer (Dama dama) Zorkóczy, Orsolya Krisztina Turi, Orsolya Wagenhoffer, Zsombor Ózsvári, László Lehotzky, Pál Pádár, Zsolt Zenke, Petra Animals (Basel) Article SIMPLE SUMMARY: Monitoring and maintaining genetic diversity is essential for the conservation of most species, including fallow deer. Various markers can be tested to estimate genetic diversity. For this purpose, microsatellites consisting of four-base-pair repetitive units are highly recommended due to their polymorphisms and comparable and reliable detection during analyses. In this study, nearly one hundred tetrameric microsatellites were collected from nine other deer species, which were tested on twenty individuals from five Hungarian fallow deer populations. As a result, 14 polymorphic markers were selected for the investigation panel, which fills a gap in the field of experts dealing with fallow deer genetics. ABSTRACT: The fallow deer (Dama dama) represents significant game management value globally, and human activities are significantly impacting the species. Besides the positive effects, these activities can threaten its existence, health, and value. The aim of the authors was to develop a tetranucleotide microsatellite panel that could be clearly interpreted and used for genetic testing of fallow deer. Such a panel did not exist until now and could be particularly useful in the field of conservation genetics and forensics. A total of 99 tetrameric microsatellites, originally designed for related deer species, were tested on 20 fallow deer individuals from five Hungarian sampling areas. Original and newly designed primers were used to amplify the microsatellite regions using previously published or optimized PCR protocols. The lengths and sequences of specific amplicons were detected using capillary electrophoresis, and the rate of polymorphism was determined. Altogether, 80 markers provided PCR products of adequate quality and quantity. Among them, 15 markers proved to be polymorphic (2–5 alleles/locus), and 14 tetrameric markers were selected for further analysis. Statistical calculations showed that the selected polymorphic microsatellites can potentially enable key individualization in many areas of wildlife and population genetics, thus protecting the species. MDPI 2023-06-23 /pmc/articles/PMC10339914/ /pubmed/37443886 http://dx.doi.org/10.3390/ani13132083 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zorkóczy, Orsolya Krisztina
Turi, Orsolya
Wagenhoffer, Zsombor
Ózsvári, László
Lehotzky, Pál
Pádár, Zsolt
Zenke, Petra
A Selection of 14 Tetrameric Microsatellite Markers for Genetic Investigations in Fallow Deer (Dama dama)
title A Selection of 14 Tetrameric Microsatellite Markers for Genetic Investigations in Fallow Deer (Dama dama)
title_full A Selection of 14 Tetrameric Microsatellite Markers for Genetic Investigations in Fallow Deer (Dama dama)
title_fullStr A Selection of 14 Tetrameric Microsatellite Markers for Genetic Investigations in Fallow Deer (Dama dama)
title_full_unstemmed A Selection of 14 Tetrameric Microsatellite Markers for Genetic Investigations in Fallow Deer (Dama dama)
title_short A Selection of 14 Tetrameric Microsatellite Markers for Genetic Investigations in Fallow Deer (Dama dama)
title_sort selection of 14 tetrameric microsatellite markers for genetic investigations in fallow deer (dama dama)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10339914/
https://www.ncbi.nlm.nih.gov/pubmed/37443886
http://dx.doi.org/10.3390/ani13132083
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