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Central and Peripheral Fatigue Evaluation during Physical Exercise in Athletic Horses by Means of Raman Spectroscopy

SIMPLE SUMMARY: In athletic horses, the evaluation of the performance levels is of major importance during training to prevent sports injuries. Raman spectroscopy is an innovative technique that allows for a rapid evaluation of biological fluids. The application of Raman spectroscopy on serum sample...

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Autores principales: Acri, Giuseppe, Testagrossa, Barbara, Piccione, Giuseppe, Arfuso, Francesca, Giudice, Elisabetta, Giannetto, Claudia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10339962/
https://www.ncbi.nlm.nih.gov/pubmed/37443998
http://dx.doi.org/10.3390/ani13132201
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author Acri, Giuseppe
Testagrossa, Barbara
Piccione, Giuseppe
Arfuso, Francesca
Giudice, Elisabetta
Giannetto, Claudia
author_facet Acri, Giuseppe
Testagrossa, Barbara
Piccione, Giuseppe
Arfuso, Francesca
Giudice, Elisabetta
Giannetto, Claudia
author_sort Acri, Giuseppe
collection PubMed
description SIMPLE SUMMARY: In athletic horses, the evaluation of the performance levels is of major importance during training to prevent sports injuries. Raman spectroscopy is an innovative technique that allows for a rapid evaluation of biological fluids. The application of Raman spectroscopy on serum samples collected from five Italian Saddle horses subjected to physical exercise confirms the utility of this technique for the assessment of the levels of serum metabolic biomarkers that cause the central and peripheral fatigue that occur in athletic horses during physical exercise. In particular, in two bands ((1300–1360) cm(−1) and (1385–1520) cm(−1)), the metabolites involved in the insurgence of fatigue were identified. Lipids and tryptophan were identified in the (1300–1360) cm(−1) band, and leucine, glycine, isoleucine, lactic acid, tripeptide, adenosine, and beta carotene were identified in the (1385–1520) cm(−1) band. The area of each evaluated sub-band was used for evaluating changes in the conformational structure of proteins in sera that may depend on exercise. In conclusion, the application of Raman spectroscopy on blood serum samples represents a useful technique for secondary-structure protein identification to investigate the metabolic changes that occur in athletic horses during physical exercise. ABSTRACT: The evaluation of the performance levels in athletic horses is of major importance to prevent sports injuries. Raman spectroscopy is an innovative technique that allows for a rapid evaluation of biomolecules in biological fluids. It also permits qualitative and quantitative sample analyses, which lead to the simultaneous determination of the components of the examined biological fluids. On the basis of this, the Raman spectroscopy technique was applied on serum samples collected from five Italian Saddle horses subjected to a standardized obstacle course preceded by a warm-up to evaluate the applicability of this technique for the assessment of central and peripheral fatigue in athletic horses. Blood samples were collected via jugular venipuncture in a vacutainer tube with a clot activator before exercise, immediately after exercise, and 30 min and 1 h after the end of the obstacle course. Observing the obtained Raman spectra, the major changes due to the experimental conditions appeared in the (1300–1360) cm(−1) and (1385–1520) cm(−1) bands. In the (1300–1360) cm(−1) band, lipids and tryptophan were identified; in the (1385–1520) cm(−1) band, leucine, glycine, isoleucine, lactic acid, tripeptide, adenosine, and beta carotene were identified. A significant effect of exercise was recorded on all the sub-bands. In particular, a change immediately after exercise versus before exercise was found. Moreover, the mean lactic concentration was positively correlated with the Raman area of the sub-band assigned to lactic acid. In this context, the application of Raman spectroscopy on blood serum samples represents a useful technique for secondary-structure protein identification to investigate the metabolic changes that occur in athletic horses during physical exercise.
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spelling pubmed-103399622023-07-14 Central and Peripheral Fatigue Evaluation during Physical Exercise in Athletic Horses by Means of Raman Spectroscopy Acri, Giuseppe Testagrossa, Barbara Piccione, Giuseppe Arfuso, Francesca Giudice, Elisabetta Giannetto, Claudia Animals (Basel) Article SIMPLE SUMMARY: In athletic horses, the evaluation of the performance levels is of major importance during training to prevent sports injuries. Raman spectroscopy is an innovative technique that allows for a rapid evaluation of biological fluids. The application of Raman spectroscopy on serum samples collected from five Italian Saddle horses subjected to physical exercise confirms the utility of this technique for the assessment of the levels of serum metabolic biomarkers that cause the central and peripheral fatigue that occur in athletic horses during physical exercise. In particular, in two bands ((1300–1360) cm(−1) and (1385–1520) cm(−1)), the metabolites involved in the insurgence of fatigue were identified. Lipids and tryptophan were identified in the (1300–1360) cm(−1) band, and leucine, glycine, isoleucine, lactic acid, tripeptide, adenosine, and beta carotene were identified in the (1385–1520) cm(−1) band. The area of each evaluated sub-band was used for evaluating changes in the conformational structure of proteins in sera that may depend on exercise. In conclusion, the application of Raman spectroscopy on blood serum samples represents a useful technique for secondary-structure protein identification to investigate the metabolic changes that occur in athletic horses during physical exercise. ABSTRACT: The evaluation of the performance levels in athletic horses is of major importance to prevent sports injuries. Raman spectroscopy is an innovative technique that allows for a rapid evaluation of biomolecules in biological fluids. It also permits qualitative and quantitative sample analyses, which lead to the simultaneous determination of the components of the examined biological fluids. On the basis of this, the Raman spectroscopy technique was applied on serum samples collected from five Italian Saddle horses subjected to a standardized obstacle course preceded by a warm-up to evaluate the applicability of this technique for the assessment of central and peripheral fatigue in athletic horses. Blood samples were collected via jugular venipuncture in a vacutainer tube with a clot activator before exercise, immediately after exercise, and 30 min and 1 h after the end of the obstacle course. Observing the obtained Raman spectra, the major changes due to the experimental conditions appeared in the (1300–1360) cm(−1) and (1385–1520) cm(−1) bands. In the (1300–1360) cm(−1) band, lipids and tryptophan were identified; in the (1385–1520) cm(−1) band, leucine, glycine, isoleucine, lactic acid, tripeptide, adenosine, and beta carotene were identified. A significant effect of exercise was recorded on all the sub-bands. In particular, a change immediately after exercise versus before exercise was found. Moreover, the mean lactic concentration was positively correlated with the Raman area of the sub-band assigned to lactic acid. In this context, the application of Raman spectroscopy on blood serum samples represents a useful technique for secondary-structure protein identification to investigate the metabolic changes that occur in athletic horses during physical exercise. MDPI 2023-07-05 /pmc/articles/PMC10339962/ /pubmed/37443998 http://dx.doi.org/10.3390/ani13132201 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Acri, Giuseppe
Testagrossa, Barbara
Piccione, Giuseppe
Arfuso, Francesca
Giudice, Elisabetta
Giannetto, Claudia
Central and Peripheral Fatigue Evaluation during Physical Exercise in Athletic Horses by Means of Raman Spectroscopy
title Central and Peripheral Fatigue Evaluation during Physical Exercise in Athletic Horses by Means of Raman Spectroscopy
title_full Central and Peripheral Fatigue Evaluation during Physical Exercise in Athletic Horses by Means of Raman Spectroscopy
title_fullStr Central and Peripheral Fatigue Evaluation during Physical Exercise in Athletic Horses by Means of Raman Spectroscopy
title_full_unstemmed Central and Peripheral Fatigue Evaluation during Physical Exercise in Athletic Horses by Means of Raman Spectroscopy
title_short Central and Peripheral Fatigue Evaluation during Physical Exercise in Athletic Horses by Means of Raman Spectroscopy
title_sort central and peripheral fatigue evaluation during physical exercise in athletic horses by means of raman spectroscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10339962/
https://www.ncbi.nlm.nih.gov/pubmed/37443998
http://dx.doi.org/10.3390/ani13132201
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