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Effects of Bacillus licheniformis on the Growth Performance, Antioxidant Capacity, Ileal Morphology, Intestinal Short Chain Fatty Acids, and Colonic Microflora in Piglets Challenged with Lipopolysaccharide

SIMPLE SUMMARY: Weaning is a critical period in sow production and induces oxidative damage, which is closely associated with diarrhea, intestinal metabolic disorders, and infections. Probiotics as substitutes for antibiotics play a beneficial role in decreasing diarrhea and improving the growth of...

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Autores principales: Cao, Guangtian, Yang, Shenglan, Wang, Huixian, Zhang, Ruiqiang, Wu, Yanping, Liu, Jinsong, Qiu, Kaifan, Dong, Yingkun, Yue, Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10340043/
https://www.ncbi.nlm.nih.gov/pubmed/37443970
http://dx.doi.org/10.3390/ani13132172
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author Cao, Guangtian
Yang, Shenglan
Wang, Huixian
Zhang, Ruiqiang
Wu, Yanping
Liu, Jinsong
Qiu, Kaifan
Dong, Yingkun
Yue, Min
author_facet Cao, Guangtian
Yang, Shenglan
Wang, Huixian
Zhang, Ruiqiang
Wu, Yanping
Liu, Jinsong
Qiu, Kaifan
Dong, Yingkun
Yue, Min
author_sort Cao, Guangtian
collection PubMed
description SIMPLE SUMMARY: Weaning is a critical period in sow production and induces oxidative damage, which is closely associated with diarrhea, intestinal metabolic disorders, and infections. Probiotics as substitutes for antibiotics play a beneficial role in decreasing diarrhea and improving the growth of weaning piglets. The intraperitoneal injection of lipopolysaccharides (LPS) has been used for inducing oxidative damage and intestinal injury in piglets. In the present study, we investigated the effects of dietary Bacillus licheniformis (BL) on production performance, serum antioxidant capacity, ileal morphology, intestinal short-chain fatty acids (SCFAs), and colonic microflora in piglets challenged with LPS. The results showed that BL supplementation improved growth performance, increased serum catalase activity, decreased malondialdehyde concentration, and increased colonic major SCFAs. Moreover, metagenome analysis showed that Prevotella species were the predominant bacteria in BL- and CBL-treated piglets. In summary, a basal diet supplemented with 10(10) CFU BL improved production performance, serum antioxidative capacity, and ileal morphology, and modulated the colonic microflora. This experiment offers theoretical support for dietary probiotic utilization in piglets to decrease weaning stress in the sow industry. ABSTRACT: The aim of the present study was to investigate the effects of Bacillus licheniformis (BL) on the growth performance, antioxidant capacity, ileal morphology, intestinal fecal short-chain fatty acids, and microflora of weaned piglets challenged with lipopolysaccharide (LPS). Piglets were assigned into three groups: basal diet (Con), a basal diet with added 10(9) CFU B. licheniformis/kg (BLl), and a basal diet with added 10(10) CFU B. licheniformis/kg (BLh). On day 28, BLh piglets were intraperitoneally injected with LPS (CBL) and sterilized saline water (BL), Con piglets were injected with LPS (LPS) and sterilized saline water (Con), with the injections being administered for three consecutive days. The average daily gain significantly increased from day 1 to day 28 and the feed: gain ratio decreased with BL supplementation compared with the Con group. Supplementation with BLl and BLh reduced the diarrhea rate in piglets. Serum catalase activity increased and malondialdehyde concentration decreased in the CBL treatment group compared with the LPS treatment group. Both BL and CBL treatments increased the ileal villus length/crypt depth ratio compared with Con and LPS treatments. BL administration significantly increased colonic propionic and isobutyric acid concentrations compared with Con treatment. Both BL and CBL piglets had significantly increased fecal acetic, propionic, and butyric acid levels compared with LPS piglets. Analysis of the colonic microbial metagenome showed that Prevotella species were the predominant bacteria in piglets treated with BL and CBL. The CBL-treated piglets had higher scores for lysine biosynthesis, arginine biosynthesis, sulfur relay system, and histidine metabolism. BL-treated piglets had higher scores for glycosaminoglycan biosynthesis-keratan sulfate, oxidative phosphorylation, and pyruvate and carbon metabolism.
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spelling pubmed-103400432023-07-14 Effects of Bacillus licheniformis on the Growth Performance, Antioxidant Capacity, Ileal Morphology, Intestinal Short Chain Fatty Acids, and Colonic Microflora in Piglets Challenged with Lipopolysaccharide Cao, Guangtian Yang, Shenglan Wang, Huixian Zhang, Ruiqiang Wu, Yanping Liu, Jinsong Qiu, Kaifan Dong, Yingkun Yue, Min Animals (Basel) Article SIMPLE SUMMARY: Weaning is a critical period in sow production and induces oxidative damage, which is closely associated with diarrhea, intestinal metabolic disorders, and infections. Probiotics as substitutes for antibiotics play a beneficial role in decreasing diarrhea and improving the growth of weaning piglets. The intraperitoneal injection of lipopolysaccharides (LPS) has been used for inducing oxidative damage and intestinal injury in piglets. In the present study, we investigated the effects of dietary Bacillus licheniformis (BL) on production performance, serum antioxidant capacity, ileal morphology, intestinal short-chain fatty acids (SCFAs), and colonic microflora in piglets challenged with LPS. The results showed that BL supplementation improved growth performance, increased serum catalase activity, decreased malondialdehyde concentration, and increased colonic major SCFAs. Moreover, metagenome analysis showed that Prevotella species were the predominant bacteria in BL- and CBL-treated piglets. In summary, a basal diet supplemented with 10(10) CFU BL improved production performance, serum antioxidative capacity, and ileal morphology, and modulated the colonic microflora. This experiment offers theoretical support for dietary probiotic utilization in piglets to decrease weaning stress in the sow industry. ABSTRACT: The aim of the present study was to investigate the effects of Bacillus licheniformis (BL) on the growth performance, antioxidant capacity, ileal morphology, intestinal fecal short-chain fatty acids, and microflora of weaned piglets challenged with lipopolysaccharide (LPS). Piglets were assigned into three groups: basal diet (Con), a basal diet with added 10(9) CFU B. licheniformis/kg (BLl), and a basal diet with added 10(10) CFU B. licheniformis/kg (BLh). On day 28, BLh piglets were intraperitoneally injected with LPS (CBL) and sterilized saline water (BL), Con piglets were injected with LPS (LPS) and sterilized saline water (Con), with the injections being administered for three consecutive days. The average daily gain significantly increased from day 1 to day 28 and the feed: gain ratio decreased with BL supplementation compared with the Con group. Supplementation with BLl and BLh reduced the diarrhea rate in piglets. Serum catalase activity increased and malondialdehyde concentration decreased in the CBL treatment group compared with the LPS treatment group. Both BL and CBL treatments increased the ileal villus length/crypt depth ratio compared with Con and LPS treatments. BL administration significantly increased colonic propionic and isobutyric acid concentrations compared with Con treatment. Both BL and CBL piglets had significantly increased fecal acetic, propionic, and butyric acid levels compared with LPS piglets. Analysis of the colonic microbial metagenome showed that Prevotella species were the predominant bacteria in piglets treated with BL and CBL. The CBL-treated piglets had higher scores for lysine biosynthesis, arginine biosynthesis, sulfur relay system, and histidine metabolism. BL-treated piglets had higher scores for glycosaminoglycan biosynthesis-keratan sulfate, oxidative phosphorylation, and pyruvate and carbon metabolism. MDPI 2023-07-01 /pmc/articles/PMC10340043/ /pubmed/37443970 http://dx.doi.org/10.3390/ani13132172 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cao, Guangtian
Yang, Shenglan
Wang, Huixian
Zhang, Ruiqiang
Wu, Yanping
Liu, Jinsong
Qiu, Kaifan
Dong, Yingkun
Yue, Min
Effects of Bacillus licheniformis on the Growth Performance, Antioxidant Capacity, Ileal Morphology, Intestinal Short Chain Fatty Acids, and Colonic Microflora in Piglets Challenged with Lipopolysaccharide
title Effects of Bacillus licheniformis on the Growth Performance, Antioxidant Capacity, Ileal Morphology, Intestinal Short Chain Fatty Acids, and Colonic Microflora in Piglets Challenged with Lipopolysaccharide
title_full Effects of Bacillus licheniformis on the Growth Performance, Antioxidant Capacity, Ileal Morphology, Intestinal Short Chain Fatty Acids, and Colonic Microflora in Piglets Challenged with Lipopolysaccharide
title_fullStr Effects of Bacillus licheniformis on the Growth Performance, Antioxidant Capacity, Ileal Morphology, Intestinal Short Chain Fatty Acids, and Colonic Microflora in Piglets Challenged with Lipopolysaccharide
title_full_unstemmed Effects of Bacillus licheniformis on the Growth Performance, Antioxidant Capacity, Ileal Morphology, Intestinal Short Chain Fatty Acids, and Colonic Microflora in Piglets Challenged with Lipopolysaccharide
title_short Effects of Bacillus licheniformis on the Growth Performance, Antioxidant Capacity, Ileal Morphology, Intestinal Short Chain Fatty Acids, and Colonic Microflora in Piglets Challenged with Lipopolysaccharide
title_sort effects of bacillus licheniformis on the growth performance, antioxidant capacity, ileal morphology, intestinal short chain fatty acids, and colonic microflora in piglets challenged with lipopolysaccharide
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10340043/
https://www.ncbi.nlm.nih.gov/pubmed/37443970
http://dx.doi.org/10.3390/ani13132172
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