Dysregulation of Inflammation, Oxidative Stress, and Glucose Metabolism-Related Genes and miRNAs in Visceral Adipose Tissue of Women with Type 2 Diabetes Mellitus

BACKGROUND: Visceral adipose tissue (VAT), which has recently been recognized as an endocrine organ, contributes to prediabetes and diabetes (T2DM) upon deregulated visceral adipocytes’ (vAds) metabolism and adipogenesis in obesity. Thus, we aimed to investigate inflammation-, oxidative stress-, and...

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Detalles Bibliográficos
Autores principales: Wróblewski, Adam, Strycharz, Justyna, Oszajca, Katarzyna, Czarny, Piotr, Świderska, Ewa, Matyjas, Tomasz, Zieleniak, Andrzej, Rucińska, Monika, Pomorski, Lech, Drzewoski, Józef, Śliwińska, Agnieszka, Szemraj, Janusz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Scientific Literature, Inc. 2023
Materias:
Clinical Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10340125/
https://www.ncbi.nlm.nih.gov/pubmed/37422695
http://dx.doi.org/10.12659/MSM.939299
Descripción
Sumario:BACKGROUND: Visceral adipose tissue (VAT), which has recently been recognized as an endocrine organ, contributes to prediabetes and diabetes (T2DM) upon deregulated visceral adipocytes’ (vAds) metabolism and adipogenesis in obesity. Thus, we aimed to investigate inflammation-, oxidative stress-, and glucose metabolism-associated genes with related miRNAs in human vAds and in VAT of subjects with glucose metabolism disorders. MATERIAL/METHODS: We analyzed expression of ATM, NFKB1, SOD2, INSR, and TIGAR, and corresponding miRNAs via PCR: i) during 3-stage-long visceral adipogenesis in normoglycemia (5.5 mM of glucose), intermittent (IH) and chronic hyperglycemia (CH) (30 mM); and ii) in VAT of subjects (34 females, 18 males) with normal glucose metabolism, impaired fasting glucose (IFG), and T2DM. RESULTS: CH and IH similarly upregulated (ATM, NFKB1, TIGAR) or downregulated (SOD2, INSR) gene expression in vAds, with a few converging expression changes of the tested miRNAs (eg, let-7g-5p/miR-145-5p/miR-21-5p). Anthropometric and biochemical parameters of the subjects encouraged us to analyze exclusively female subjects, which demonstrated transactivated NFKB1, TIGAR, and miR-10b-5p/-132-3p/-20a-5p/-21-5p/-26a-5p only in T2DM. The upregulated molecules (excluding miR-10b-5p and miR-20a-5p) were also positively correlated with parameters related to glucose metabolism. CONCLUSIONS: The studied genes may be subjected to miRNA interferences and hyperglycemic memory upon hyperglycemia in vAds. VAT of women with T2DM, but not with IFG, manifested a set of transactivated miRNAs, and a molecular dysregulation of TIGAR and NFKB1, which may support enhanced inflammation, oxidative stress, and disturbed glucose metabolism. Our results shed light on epigenetic and molecular VAT disturbances in glucose metabolism disorders, but more research is needed to further assess their biological relevance.

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