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Detection of Specific Volatile Organic Compounds in Tribolium castaneum (Herbst) by Solid-Phase Microextraction and Gas Chromatography-Mass Spectrometry
The red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae), is a major storage pest that could lead to a wide range of damage. Its secretions have a significant impact on the quality of stored grain and food, leading to serious food safety problems such as grain spoilage and food...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10340164/ https://www.ncbi.nlm.nih.gov/pubmed/37444222 http://dx.doi.org/10.3390/foods12132484 |
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author | Han, Shaoyun He, Ke An, Jing Qiao, Mengmeng Ke, Runhui Wang, Xiao Xu, Yang Tang, Xiuying |
author_facet | Han, Shaoyun He, Ke An, Jing Qiao, Mengmeng Ke, Runhui Wang, Xiao Xu, Yang Tang, Xiuying |
author_sort | Han, Shaoyun |
collection | PubMed |
description | The red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae), is a major storage pest that could lead to a wide range of damage. Its secretions have a significant impact on the quality of stored grain and food, leading to serious food safety problems such as grain spoilage and food carcinogenesis. This study investigates new detection techniques for grain storage pests to improve grain insect detection in China. The primary volatile organic chemicals (VOCs) in these secretions are identified using headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS). The specific VOCs that are unique to T. castaneum are selected as criteria for determining the presence of T. castaneum in the granary. To obtain more specific VOCs, experiments were designed for the analysis of T. castaneum samples under different extraction times, two types of SPME fibers and two GC-MS devices of different manufacturers. The experimental results showed that 12 VOCs were detected at relatively high levels, seven of which were common and which were not detected in other grains and grain insects. The seven compounds are 1-pentadecene, 2-methyl-p-benzoquinone, 2-ethyl-p-benzoquinone, 1-hexadecene, cis-9-tetradecen-1-ol, m-cresol and paeonol. These seven compounds can be used as volatile markers to identify the presence of T. castaneum, which could serve as a research foundation for the creation of new techniques for T. castaneum monitoring. |
format | Online Article Text |
id | pubmed-10340164 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-103401642023-07-14 Detection of Specific Volatile Organic Compounds in Tribolium castaneum (Herbst) by Solid-Phase Microextraction and Gas Chromatography-Mass Spectrometry Han, Shaoyun He, Ke An, Jing Qiao, Mengmeng Ke, Runhui Wang, Xiao Xu, Yang Tang, Xiuying Foods Article The red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae), is a major storage pest that could lead to a wide range of damage. Its secretions have a significant impact on the quality of stored grain and food, leading to serious food safety problems such as grain spoilage and food carcinogenesis. This study investigates new detection techniques for grain storage pests to improve grain insect detection in China. The primary volatile organic chemicals (VOCs) in these secretions are identified using headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS). The specific VOCs that are unique to T. castaneum are selected as criteria for determining the presence of T. castaneum in the granary. To obtain more specific VOCs, experiments were designed for the analysis of T. castaneum samples under different extraction times, two types of SPME fibers and two GC-MS devices of different manufacturers. The experimental results showed that 12 VOCs were detected at relatively high levels, seven of which were common and which were not detected in other grains and grain insects. The seven compounds are 1-pentadecene, 2-methyl-p-benzoquinone, 2-ethyl-p-benzoquinone, 1-hexadecene, cis-9-tetradecen-1-ol, m-cresol and paeonol. These seven compounds can be used as volatile markers to identify the presence of T. castaneum, which could serve as a research foundation for the creation of new techniques for T. castaneum monitoring. MDPI 2023-06-25 /pmc/articles/PMC10340164/ /pubmed/37444222 http://dx.doi.org/10.3390/foods12132484 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Han, Shaoyun He, Ke An, Jing Qiao, Mengmeng Ke, Runhui Wang, Xiao Xu, Yang Tang, Xiuying Detection of Specific Volatile Organic Compounds in Tribolium castaneum (Herbst) by Solid-Phase Microextraction and Gas Chromatography-Mass Spectrometry |
title | Detection of Specific Volatile Organic Compounds in Tribolium castaneum (Herbst) by Solid-Phase Microextraction and Gas Chromatography-Mass Spectrometry |
title_full | Detection of Specific Volatile Organic Compounds in Tribolium castaneum (Herbst) by Solid-Phase Microextraction and Gas Chromatography-Mass Spectrometry |
title_fullStr | Detection of Specific Volatile Organic Compounds in Tribolium castaneum (Herbst) by Solid-Phase Microextraction and Gas Chromatography-Mass Spectrometry |
title_full_unstemmed | Detection of Specific Volatile Organic Compounds in Tribolium castaneum (Herbst) by Solid-Phase Microextraction and Gas Chromatography-Mass Spectrometry |
title_short | Detection of Specific Volatile Organic Compounds in Tribolium castaneum (Herbst) by Solid-Phase Microextraction and Gas Chromatography-Mass Spectrometry |
title_sort | detection of specific volatile organic compounds in tribolium castaneum (herbst) by solid-phase microextraction and gas chromatography-mass spectrometry |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10340164/ https://www.ncbi.nlm.nih.gov/pubmed/37444222 http://dx.doi.org/10.3390/foods12132484 |
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