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Patterned Arteriole-Scale Vessels Enhance Engraftment, Perfusion, and Vessel Branching Hierarchy of Engineered Human Myocardium for Heart Regeneration

Heart regeneration after myocardial infarction (MI) using human stem cell-derived cardiomyocytes (CMs) is rapidly accelerating with large animal and human clinical trials. However, vascularization methods to support the engraftment, survival, and development of implanted CMs in the ischemic environm...

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Autores principales: Kant, Rajeev J., Dwyer, Kiera D., Lee, Jang-Hoon, Polucha, Collin, Kobayashi, Momoka, Pyon, Stephen, Soepriatna, Arvin H., Lee, Jonghwan, Coulombe, Kareen L. K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10340601/
https://www.ncbi.nlm.nih.gov/pubmed/37443731
http://dx.doi.org/10.3390/cells12131698
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author Kant, Rajeev J.
Dwyer, Kiera D.
Lee, Jang-Hoon
Polucha, Collin
Kobayashi, Momoka
Pyon, Stephen
Soepriatna, Arvin H.
Lee, Jonghwan
Coulombe, Kareen L. K.
author_facet Kant, Rajeev J.
Dwyer, Kiera D.
Lee, Jang-Hoon
Polucha, Collin
Kobayashi, Momoka
Pyon, Stephen
Soepriatna, Arvin H.
Lee, Jonghwan
Coulombe, Kareen L. K.
author_sort Kant, Rajeev J.
collection PubMed
description Heart regeneration after myocardial infarction (MI) using human stem cell-derived cardiomyocytes (CMs) is rapidly accelerating with large animal and human clinical trials. However, vascularization methods to support the engraftment, survival, and development of implanted CMs in the ischemic environment of the infarcted heart remain a key and timely challenge. To this end, we developed a dual remuscularization-revascularization therapy that is evaluated in a rat model of ischemia-reperfusion MI. This study details the differentiation of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) for engineering cardiac tissue containing patterned engineered vessels 400 μm in diameter. Vascularized engineered human myocardial tissues (vEHMs) are cultured in static conditions or perfused in vitro prior to implantation and evaluated after two weeks. Immunohistochemical staining indicates improved engraftment of hiPSC-CMs in in vitro-perfused vEHMs with greater expression of SMA+ vessels and evidence of inosculation. Three-dimensional vascular reconstructions reveal less tortuous and larger intra-implant vessels, as well as an improved branching hierarchy in in vitro-perfused vEHMs relative to non-perfused controls. Exploratory RNA sequencing of explanted vEHMs supports the hypothesis that co-revascularization impacts hiPSC-CM development in vivo. Our approach provides a strong foundation to enhance vEHM integration, develop hierarchical vascular perfusion, and maximize hiPSC-CM engraftment for future regenerative therapy.
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spelling pubmed-103406012023-07-14 Patterned Arteriole-Scale Vessels Enhance Engraftment, Perfusion, and Vessel Branching Hierarchy of Engineered Human Myocardium for Heart Regeneration Kant, Rajeev J. Dwyer, Kiera D. Lee, Jang-Hoon Polucha, Collin Kobayashi, Momoka Pyon, Stephen Soepriatna, Arvin H. Lee, Jonghwan Coulombe, Kareen L. K. Cells Article Heart regeneration after myocardial infarction (MI) using human stem cell-derived cardiomyocytes (CMs) is rapidly accelerating with large animal and human clinical trials. However, vascularization methods to support the engraftment, survival, and development of implanted CMs in the ischemic environment of the infarcted heart remain a key and timely challenge. To this end, we developed a dual remuscularization-revascularization therapy that is evaluated in a rat model of ischemia-reperfusion MI. This study details the differentiation of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) for engineering cardiac tissue containing patterned engineered vessels 400 μm in diameter. Vascularized engineered human myocardial tissues (vEHMs) are cultured in static conditions or perfused in vitro prior to implantation and evaluated after two weeks. Immunohistochemical staining indicates improved engraftment of hiPSC-CMs in in vitro-perfused vEHMs with greater expression of SMA+ vessels and evidence of inosculation. Three-dimensional vascular reconstructions reveal less tortuous and larger intra-implant vessels, as well as an improved branching hierarchy in in vitro-perfused vEHMs relative to non-perfused controls. Exploratory RNA sequencing of explanted vEHMs supports the hypothesis that co-revascularization impacts hiPSC-CM development in vivo. Our approach provides a strong foundation to enhance vEHM integration, develop hierarchical vascular perfusion, and maximize hiPSC-CM engraftment for future regenerative therapy. MDPI 2023-06-23 /pmc/articles/PMC10340601/ /pubmed/37443731 http://dx.doi.org/10.3390/cells12131698 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kant, Rajeev J.
Dwyer, Kiera D.
Lee, Jang-Hoon
Polucha, Collin
Kobayashi, Momoka
Pyon, Stephen
Soepriatna, Arvin H.
Lee, Jonghwan
Coulombe, Kareen L. K.
Patterned Arteriole-Scale Vessels Enhance Engraftment, Perfusion, and Vessel Branching Hierarchy of Engineered Human Myocardium for Heart Regeneration
title Patterned Arteriole-Scale Vessels Enhance Engraftment, Perfusion, and Vessel Branching Hierarchy of Engineered Human Myocardium for Heart Regeneration
title_full Patterned Arteriole-Scale Vessels Enhance Engraftment, Perfusion, and Vessel Branching Hierarchy of Engineered Human Myocardium for Heart Regeneration
title_fullStr Patterned Arteriole-Scale Vessels Enhance Engraftment, Perfusion, and Vessel Branching Hierarchy of Engineered Human Myocardium for Heart Regeneration
title_full_unstemmed Patterned Arteriole-Scale Vessels Enhance Engraftment, Perfusion, and Vessel Branching Hierarchy of Engineered Human Myocardium for Heart Regeneration
title_short Patterned Arteriole-Scale Vessels Enhance Engraftment, Perfusion, and Vessel Branching Hierarchy of Engineered Human Myocardium for Heart Regeneration
title_sort patterned arteriole-scale vessels enhance engraftment, perfusion, and vessel branching hierarchy of engineered human myocardium for heart regeneration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10340601/
https://www.ncbi.nlm.nih.gov/pubmed/37443731
http://dx.doi.org/10.3390/cells12131698
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