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A Multivariant Surrogate Neutralization Assay Identifies Variant-Specific Neutralizing Antibody Profiles in Primary SARS-CoV-2 Omicron Infection
Primary infection with the Omicron variant of Severe Acute Respiratory Syndrome Corona Virus 2 (SARS-CoV-2) can be serologically identified with distinct profiles of neutralizing antibodies (nAbs), as indicated by high titers against the Omicron variant and low titers against the ancestral wild-type...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10341213/ https://www.ncbi.nlm.nih.gov/pubmed/37443672 http://dx.doi.org/10.3390/diagnostics13132278 |
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author | Springer, David Niklas Traugott, Marianna Reuberger, Elisabeth Kothbauer, Klaus Benjamin Borsodi, Christian Nägeli, Michelle Oelschlägel, Theresa Kelani, Hasan Lammel, Oliver Deutsch, Josef Puchhammer-Stöckl, Elisabeth Höltl, Eva Aberle, Judith Helene Stiasny, Karin Weseslindtner, Lukas |
author_facet | Springer, David Niklas Traugott, Marianna Reuberger, Elisabeth Kothbauer, Klaus Benjamin Borsodi, Christian Nägeli, Michelle Oelschlägel, Theresa Kelani, Hasan Lammel, Oliver Deutsch, Josef Puchhammer-Stöckl, Elisabeth Höltl, Eva Aberle, Judith Helene Stiasny, Karin Weseslindtner, Lukas |
author_sort | Springer, David Niklas |
collection | PubMed |
description | Primary infection with the Omicron variant of Severe Acute Respiratory Syndrome Corona Virus 2 (SARS-CoV-2) can be serologically identified with distinct profiles of neutralizing antibodies (nAbs), as indicated by high titers against the Omicron variant and low titers against the ancestral wild-type (WT). Here, we evaluated whether a novel surrogate virus neutralization assay (sVNT) that simultaneously quantifies the binding inhibition of angiotensin-converting enzyme 2 (ACE2) to the proteins of the WT- and Omicron-specific receptor-binding domains (RBDs) can identify nAb profiles after primary Omicron infection with accuracy similar to that of variant-specific live-virus neutralization tests (NTs). Therefore, we comparatively tested 205 samples from individuals after primary infection with the Omicron variant and the WT, and vaccinated subjects with or without Omicron breakthrough infections. Indeed, variant-specific RBD-ACE2 binding inhibition levels significantly correlated with respective NT titers (p < 0.0001, Spearman’s r = 0.92 and r = 0.80 for WT and Omicron, respectively). In addition, samples from individuals after primary Omicron infection were securely identified with the sVNT according to their distinctive nAb profiles (area under the curve = 0.99; sensitivity: 97.2%; specificity: 97.84%). Thus, when laborious live-virus NTs are not feasible, the novel sVNT we evaluated in this study may serve as an acceptable substitute for the serological identification of individuals with primary Omicron infection. |
format | Online Article Text |
id | pubmed-10341213 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-103412132023-07-14 A Multivariant Surrogate Neutralization Assay Identifies Variant-Specific Neutralizing Antibody Profiles in Primary SARS-CoV-2 Omicron Infection Springer, David Niklas Traugott, Marianna Reuberger, Elisabeth Kothbauer, Klaus Benjamin Borsodi, Christian Nägeli, Michelle Oelschlägel, Theresa Kelani, Hasan Lammel, Oliver Deutsch, Josef Puchhammer-Stöckl, Elisabeth Höltl, Eva Aberle, Judith Helene Stiasny, Karin Weseslindtner, Lukas Diagnostics (Basel) Brief Report Primary infection with the Omicron variant of Severe Acute Respiratory Syndrome Corona Virus 2 (SARS-CoV-2) can be serologically identified with distinct profiles of neutralizing antibodies (nAbs), as indicated by high titers against the Omicron variant and low titers against the ancestral wild-type (WT). Here, we evaluated whether a novel surrogate virus neutralization assay (sVNT) that simultaneously quantifies the binding inhibition of angiotensin-converting enzyme 2 (ACE2) to the proteins of the WT- and Omicron-specific receptor-binding domains (RBDs) can identify nAb profiles after primary Omicron infection with accuracy similar to that of variant-specific live-virus neutralization tests (NTs). Therefore, we comparatively tested 205 samples from individuals after primary infection with the Omicron variant and the WT, and vaccinated subjects with or without Omicron breakthrough infections. Indeed, variant-specific RBD-ACE2 binding inhibition levels significantly correlated with respective NT titers (p < 0.0001, Spearman’s r = 0.92 and r = 0.80 for WT and Omicron, respectively). In addition, samples from individuals after primary Omicron infection were securely identified with the sVNT according to their distinctive nAb profiles (area under the curve = 0.99; sensitivity: 97.2%; specificity: 97.84%). Thus, when laborious live-virus NTs are not feasible, the novel sVNT we evaluated in this study may serve as an acceptable substitute for the serological identification of individuals with primary Omicron infection. MDPI 2023-07-05 /pmc/articles/PMC10341213/ /pubmed/37443672 http://dx.doi.org/10.3390/diagnostics13132278 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Brief Report Springer, David Niklas Traugott, Marianna Reuberger, Elisabeth Kothbauer, Klaus Benjamin Borsodi, Christian Nägeli, Michelle Oelschlägel, Theresa Kelani, Hasan Lammel, Oliver Deutsch, Josef Puchhammer-Stöckl, Elisabeth Höltl, Eva Aberle, Judith Helene Stiasny, Karin Weseslindtner, Lukas A Multivariant Surrogate Neutralization Assay Identifies Variant-Specific Neutralizing Antibody Profiles in Primary SARS-CoV-2 Omicron Infection |
title | A Multivariant Surrogate Neutralization Assay Identifies Variant-Specific Neutralizing Antibody Profiles in Primary SARS-CoV-2 Omicron Infection |
title_full | A Multivariant Surrogate Neutralization Assay Identifies Variant-Specific Neutralizing Antibody Profiles in Primary SARS-CoV-2 Omicron Infection |
title_fullStr | A Multivariant Surrogate Neutralization Assay Identifies Variant-Specific Neutralizing Antibody Profiles in Primary SARS-CoV-2 Omicron Infection |
title_full_unstemmed | A Multivariant Surrogate Neutralization Assay Identifies Variant-Specific Neutralizing Antibody Profiles in Primary SARS-CoV-2 Omicron Infection |
title_short | A Multivariant Surrogate Neutralization Assay Identifies Variant-Specific Neutralizing Antibody Profiles in Primary SARS-CoV-2 Omicron Infection |
title_sort | multivariant surrogate neutralization assay identifies variant-specific neutralizing antibody profiles in primary sars-cov-2 omicron infection |
topic | Brief Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10341213/ https://www.ncbi.nlm.nih.gov/pubmed/37443672 http://dx.doi.org/10.3390/diagnostics13132278 |
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