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Disruption of Endochondral Ossification and Extracellular Matrix Maturation in an Ex Vivo Rat Femur Organotypic Slice Model Due to Growth Plate Injury

Postnatal bone fractures of the growth plate (GP) are often associated with regenerative complications such as growth impairment. In order to understand the underlying processes of trauma-associated growth impairment within postnatal bone, an ex vivo rat femur slice model was developed. To achieve t...

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Autores principales: Etschmaier, Vanessa, Üçal, Muammer, Lohberger, Birgit, Absenger-Novak, Markus, Kolb, Dagmar, Weinberg, Annelie, Schäfer, Ute
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10341345/
https://www.ncbi.nlm.nih.gov/pubmed/37443722
http://dx.doi.org/10.3390/cells12131687
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author Etschmaier, Vanessa
Üçal, Muammer
Lohberger, Birgit
Absenger-Novak, Markus
Kolb, Dagmar
Weinberg, Annelie
Schäfer, Ute
author_facet Etschmaier, Vanessa
Üçal, Muammer
Lohberger, Birgit
Absenger-Novak, Markus
Kolb, Dagmar
Weinberg, Annelie
Schäfer, Ute
author_sort Etschmaier, Vanessa
collection PubMed
description Postnatal bone fractures of the growth plate (GP) are often associated with regenerative complications such as growth impairment. In order to understand the underlying processes of trauma-associated growth impairment within postnatal bone, an ex vivo rat femur slice model was developed. To achieve this, a 2 mm horizontal cut was made through the GP of rat femur prior to the organotypic culture being cultivated for 15 days in vitro. Histological analysis showed disrupted endochondral ossification, including disordered architecture, increased chondrocyte metabolic activity, and a loss of hypertrophic zone throughout the distal femur. Furthermore, altered expression patterns of Col2α1, Acan, and ColX, and increased chondrocyte metabolic activity in the TZ and MZ at day 7 and day 15 postinjury were observed. STEM revealed the presence of stem cells, fibroblasts, and chondrocytes within the injury site at day 7. In summary, the findings of this study suggest that the ex vivo organotypic GP injury model could be a valuable tool for investigating the underlying mechanisms of GP regeneration post-trauma, as well as other tissue engineering and disease studies.
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spelling pubmed-103413452023-07-14 Disruption of Endochondral Ossification and Extracellular Matrix Maturation in an Ex Vivo Rat Femur Organotypic Slice Model Due to Growth Plate Injury Etschmaier, Vanessa Üçal, Muammer Lohberger, Birgit Absenger-Novak, Markus Kolb, Dagmar Weinberg, Annelie Schäfer, Ute Cells Article Postnatal bone fractures of the growth plate (GP) are often associated with regenerative complications such as growth impairment. In order to understand the underlying processes of trauma-associated growth impairment within postnatal bone, an ex vivo rat femur slice model was developed. To achieve this, a 2 mm horizontal cut was made through the GP of rat femur prior to the organotypic culture being cultivated for 15 days in vitro. Histological analysis showed disrupted endochondral ossification, including disordered architecture, increased chondrocyte metabolic activity, and a loss of hypertrophic zone throughout the distal femur. Furthermore, altered expression patterns of Col2α1, Acan, and ColX, and increased chondrocyte metabolic activity in the TZ and MZ at day 7 and day 15 postinjury were observed. STEM revealed the presence of stem cells, fibroblasts, and chondrocytes within the injury site at day 7. In summary, the findings of this study suggest that the ex vivo organotypic GP injury model could be a valuable tool for investigating the underlying mechanisms of GP regeneration post-trauma, as well as other tissue engineering and disease studies. MDPI 2023-06-22 /pmc/articles/PMC10341345/ /pubmed/37443722 http://dx.doi.org/10.3390/cells12131687 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Etschmaier, Vanessa
Üçal, Muammer
Lohberger, Birgit
Absenger-Novak, Markus
Kolb, Dagmar
Weinberg, Annelie
Schäfer, Ute
Disruption of Endochondral Ossification and Extracellular Matrix Maturation in an Ex Vivo Rat Femur Organotypic Slice Model Due to Growth Plate Injury
title Disruption of Endochondral Ossification and Extracellular Matrix Maturation in an Ex Vivo Rat Femur Organotypic Slice Model Due to Growth Plate Injury
title_full Disruption of Endochondral Ossification and Extracellular Matrix Maturation in an Ex Vivo Rat Femur Organotypic Slice Model Due to Growth Plate Injury
title_fullStr Disruption of Endochondral Ossification and Extracellular Matrix Maturation in an Ex Vivo Rat Femur Organotypic Slice Model Due to Growth Plate Injury
title_full_unstemmed Disruption of Endochondral Ossification and Extracellular Matrix Maturation in an Ex Vivo Rat Femur Organotypic Slice Model Due to Growth Plate Injury
title_short Disruption of Endochondral Ossification and Extracellular Matrix Maturation in an Ex Vivo Rat Femur Organotypic Slice Model Due to Growth Plate Injury
title_sort disruption of endochondral ossification and extracellular matrix maturation in an ex vivo rat femur organotypic slice model due to growth plate injury
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10341345/
https://www.ncbi.nlm.nih.gov/pubmed/37443722
http://dx.doi.org/10.3390/cells12131687
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