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Atomic Force Microscopy and High-Resolution Spectrophotometry for Study of Anoxemia and Normoxemia in Model Experiment In Vitro

The oxygen content in the blood may decrease under the influence of various physicochemical factors and different diseases. The state of hypoxemia is especially dangerous for critically ill patients. In this paper, we describe and analyze the changes in the characteristics of red blood cells (RBCs)...

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Autores principales: Kozlova, Elena, Sherstyukova, Ekaterina, Sergunova, Viktoria, Grechko, Andrey, Kuzovlev, Artem, Lyapunova, Snezhanna, Inozemtsev, Vladimir, Kozlov, Aleksandr, Chernysh, Aleksandr
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10341442/
https://www.ncbi.nlm.nih.gov/pubmed/37446221
http://dx.doi.org/10.3390/ijms241311043
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author Kozlova, Elena
Sherstyukova, Ekaterina
Sergunova, Viktoria
Grechko, Andrey
Kuzovlev, Artem
Lyapunova, Snezhanna
Inozemtsev, Vladimir
Kozlov, Aleksandr
Chernysh, Aleksandr
author_facet Kozlova, Elena
Sherstyukova, Ekaterina
Sergunova, Viktoria
Grechko, Andrey
Kuzovlev, Artem
Lyapunova, Snezhanna
Inozemtsev, Vladimir
Kozlov, Aleksandr
Chernysh, Aleksandr
author_sort Kozlova, Elena
collection PubMed
description The oxygen content in the blood may decrease under the influence of various physicochemical factors and different diseases. The state of hypoxemia is especially dangerous for critically ill patients. In this paper, we describe and analyze the changes in the characteristics of red blood cells (RBCs) with decreasing levels of oxygen in the RBC suspension from normoxemia to hypoxemia/anoxemia in an in vitro model experiment. The RBCs were stored in hypoxemia/anoxemia and normoxemia conditions in closed and open tubes correspondingly. For the quantitative study of RBC parameter changes, we used atomic force microscopy, digital spectrophotometry, and nonlinear curve fitting of the optical spectra. In both closed and open tubes, at the end of the storage period by day 29, only 2% of discocytes remained, and mainly irreversible types, such as microspherocytes and ghosts, were observed. RBC hemolysis occurred at a level of 25–30%. Addition of the storage solution, depending on the concentration, changed the influence of hypoxemia on RBCs. The reversibility of the change in hemoglobin derivatives was checked. Based on the experimental data and model approach, we assume that there is an optimal level of hypoxemia at which the imbalance between the oxidative and antioxidant systems, the rate of formation of reactive oxygen species, and, accordingly, the disturbances in RBCs, will be minimal.
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spelling pubmed-103414422023-07-14 Atomic Force Microscopy and High-Resolution Spectrophotometry for Study of Anoxemia and Normoxemia in Model Experiment In Vitro Kozlova, Elena Sherstyukova, Ekaterina Sergunova, Viktoria Grechko, Andrey Kuzovlev, Artem Lyapunova, Snezhanna Inozemtsev, Vladimir Kozlov, Aleksandr Chernysh, Aleksandr Int J Mol Sci Article The oxygen content in the blood may decrease under the influence of various physicochemical factors and different diseases. The state of hypoxemia is especially dangerous for critically ill patients. In this paper, we describe and analyze the changes in the characteristics of red blood cells (RBCs) with decreasing levels of oxygen in the RBC suspension from normoxemia to hypoxemia/anoxemia in an in vitro model experiment. The RBCs were stored in hypoxemia/anoxemia and normoxemia conditions in closed and open tubes correspondingly. For the quantitative study of RBC parameter changes, we used atomic force microscopy, digital spectrophotometry, and nonlinear curve fitting of the optical spectra. In both closed and open tubes, at the end of the storage period by day 29, only 2% of discocytes remained, and mainly irreversible types, such as microspherocytes and ghosts, were observed. RBC hemolysis occurred at a level of 25–30%. Addition of the storage solution, depending on the concentration, changed the influence of hypoxemia on RBCs. The reversibility of the change in hemoglobin derivatives was checked. Based on the experimental data and model approach, we assume that there is an optimal level of hypoxemia at which the imbalance between the oxidative and antioxidant systems, the rate of formation of reactive oxygen species, and, accordingly, the disturbances in RBCs, will be minimal. MDPI 2023-07-03 /pmc/articles/PMC10341442/ /pubmed/37446221 http://dx.doi.org/10.3390/ijms241311043 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kozlova, Elena
Sherstyukova, Ekaterina
Sergunova, Viktoria
Grechko, Andrey
Kuzovlev, Artem
Lyapunova, Snezhanna
Inozemtsev, Vladimir
Kozlov, Aleksandr
Chernysh, Aleksandr
Atomic Force Microscopy and High-Resolution Spectrophotometry for Study of Anoxemia and Normoxemia in Model Experiment In Vitro
title Atomic Force Microscopy and High-Resolution Spectrophotometry for Study of Anoxemia and Normoxemia in Model Experiment In Vitro
title_full Atomic Force Microscopy and High-Resolution Spectrophotometry for Study of Anoxemia and Normoxemia in Model Experiment In Vitro
title_fullStr Atomic Force Microscopy and High-Resolution Spectrophotometry for Study of Anoxemia and Normoxemia in Model Experiment In Vitro
title_full_unstemmed Atomic Force Microscopy and High-Resolution Spectrophotometry for Study of Anoxemia and Normoxemia in Model Experiment In Vitro
title_short Atomic Force Microscopy and High-Resolution Spectrophotometry for Study of Anoxemia and Normoxemia in Model Experiment In Vitro
title_sort atomic force microscopy and high-resolution spectrophotometry for study of anoxemia and normoxemia in model experiment in vitro
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10341442/
https://www.ncbi.nlm.nih.gov/pubmed/37446221
http://dx.doi.org/10.3390/ijms241311043
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