Cargando…

Metabolic Biomarkers Differentiate a Surgical Intervertebral Disc from a Nonsurgical Intervertebral Disc

Background: Degeneration of the intervertebral disc (IVD) is caused by disturbances in metabolic processes, which lead to structural disorders. The aim of this report is to analyze metabolic disorders in the degeneration process by comparing control discs with degenerated discs. In our research on t...

Descripción completa

Detalles Bibliográficos
Autores principales: Toczylowska, Beata, Woznica, Michal, Zieminska, Elzbieta, Krolicki, Leszek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10341471/
https://www.ncbi.nlm.nih.gov/pubmed/37445750
http://dx.doi.org/10.3390/ijms241310572
_version_ 1785072269830127616
author Toczylowska, Beata
Woznica, Michal
Zieminska, Elzbieta
Krolicki, Leszek
author_facet Toczylowska, Beata
Woznica, Michal
Zieminska, Elzbieta
Krolicki, Leszek
author_sort Toczylowska, Beata
collection PubMed
description Background: Degeneration of the intervertebral disc (IVD) is caused by disturbances in metabolic processes, which lead to structural disorders. The aim of this report is to analyze metabolic disorders in the degeneration process by comparing control discs with degenerated discs. In our research on the nucleus pulposus (NP), we used NMR spectroscopy of extracts of hydrophilic and hydrophobic compounds of the tissue. Methods: Nuclear magnetic resonance (NMR) spectroscopy allows the study of biochemistry and cellular metabolism in vitro. Hydrophilic and hydrophobic compounds were extracted from the NP of the intervertebral disc. In the NMR spectra, metabolites were identified and quantitatively analyzed. The results of our research indicate disturbances in the biosynthesis and metabolism of cholesterol, the biosynthesis and degradation of various fatty acid groups, ketone bodies, or lysine, and the metabolism of glycerophospholipids, purines, glycine, inositol, galactose, alanine, glutamate, and pyruvate in the biosynthesis of valine and isoleucine, leucine. All these disorders indicate pathomechanisms related to oxidative stress, energy, neurotransmission disturbances, and disturbances in the structure and functioning of cell membranes, inflammation, or chronic pain generators. Conclusions: NMR spectroscopy allows the identification of metabolites differentiating surgical from nonsurgical discs. These data may provide guidance in in vivo MRS studies in assessing the severity of lesions of the disc.
format Online
Article
Text
id pubmed-10341471
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-103414712023-07-14 Metabolic Biomarkers Differentiate a Surgical Intervertebral Disc from a Nonsurgical Intervertebral Disc Toczylowska, Beata Woznica, Michal Zieminska, Elzbieta Krolicki, Leszek Int J Mol Sci Case Report Background: Degeneration of the intervertebral disc (IVD) is caused by disturbances in metabolic processes, which lead to structural disorders. The aim of this report is to analyze metabolic disorders in the degeneration process by comparing control discs with degenerated discs. In our research on the nucleus pulposus (NP), we used NMR spectroscopy of extracts of hydrophilic and hydrophobic compounds of the tissue. Methods: Nuclear magnetic resonance (NMR) spectroscopy allows the study of biochemistry and cellular metabolism in vitro. Hydrophilic and hydrophobic compounds were extracted from the NP of the intervertebral disc. In the NMR spectra, metabolites were identified and quantitatively analyzed. The results of our research indicate disturbances in the biosynthesis and metabolism of cholesterol, the biosynthesis and degradation of various fatty acid groups, ketone bodies, or lysine, and the metabolism of glycerophospholipids, purines, glycine, inositol, galactose, alanine, glutamate, and pyruvate in the biosynthesis of valine and isoleucine, leucine. All these disorders indicate pathomechanisms related to oxidative stress, energy, neurotransmission disturbances, and disturbances in the structure and functioning of cell membranes, inflammation, or chronic pain generators. Conclusions: NMR spectroscopy allows the identification of metabolites differentiating surgical from nonsurgical discs. These data may provide guidance in in vivo MRS studies in assessing the severity of lesions of the disc. MDPI 2023-06-24 /pmc/articles/PMC10341471/ /pubmed/37445750 http://dx.doi.org/10.3390/ijms241310572 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Case Report
Toczylowska, Beata
Woznica, Michal
Zieminska, Elzbieta
Krolicki, Leszek
Metabolic Biomarkers Differentiate a Surgical Intervertebral Disc from a Nonsurgical Intervertebral Disc
title Metabolic Biomarkers Differentiate a Surgical Intervertebral Disc from a Nonsurgical Intervertebral Disc
title_full Metabolic Biomarkers Differentiate a Surgical Intervertebral Disc from a Nonsurgical Intervertebral Disc
title_fullStr Metabolic Biomarkers Differentiate a Surgical Intervertebral Disc from a Nonsurgical Intervertebral Disc
title_full_unstemmed Metabolic Biomarkers Differentiate a Surgical Intervertebral Disc from a Nonsurgical Intervertebral Disc
title_short Metabolic Biomarkers Differentiate a Surgical Intervertebral Disc from a Nonsurgical Intervertebral Disc
title_sort metabolic biomarkers differentiate a surgical intervertebral disc from a nonsurgical intervertebral disc
topic Case Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10341471/
https://www.ncbi.nlm.nih.gov/pubmed/37445750
http://dx.doi.org/10.3390/ijms241310572
work_keys_str_mv AT toczylowskabeata metabolicbiomarkersdifferentiateasurgicalintervertebraldiscfromanonsurgicalintervertebraldisc
AT woznicamichal metabolicbiomarkersdifferentiateasurgicalintervertebraldiscfromanonsurgicalintervertebraldisc
AT zieminskaelzbieta metabolicbiomarkersdifferentiateasurgicalintervertebraldiscfromanonsurgicalintervertebraldisc
AT krolickileszek metabolicbiomarkersdifferentiateasurgicalintervertebraldiscfromanonsurgicalintervertebraldisc