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Transcription Pattern of Neurotrophic Factors and Their Receptors in Adult Zebrafish Spinal Cord
In vertebrates, neurotrophins and their receptors play a fundamental role in the central and peripheral nervous systems. Several studies reported that each neurotrophin/receptor signalling pathway can perform various functions during axon development, neuronal growth, and plasticity. Previous invest...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10341495/ https://www.ncbi.nlm.nih.gov/pubmed/37446129 http://dx.doi.org/10.3390/ijms241310953 |
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author | Cacialli, Pietro Ricci, Serena Lazzari, Maurizio Milani, Liliana Franceschini, Valeria |
author_facet | Cacialli, Pietro Ricci, Serena Lazzari, Maurizio Milani, Liliana Franceschini, Valeria |
author_sort | Cacialli, Pietro |
collection | PubMed |
description | In vertebrates, neurotrophins and their receptors play a fundamental role in the central and peripheral nervous systems. Several studies reported that each neurotrophin/receptor signalling pathway can perform various functions during axon development, neuronal growth, and plasticity. Previous investigations in some fish species have identified neurotrophins and their receptors in the spinal cord under physiological conditions and after injuries, highlighting their potential role during regeneration. In our study, for the first time, we used an excellent animal model, the zebrafish (Danio rerio), to compare the mRNA localization patterns of neurotrophins and receptors in the spinal cord. We quantified the levels of mRNA using qPCR, and identified the transcription pattern of each neurotrophin/receptor pathway via in situ hybridization. Our data show that ngf/trka are the most transcribed members in the adult zebrafish spinal cord. |
format | Online Article Text |
id | pubmed-10341495 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-103414952023-07-14 Transcription Pattern of Neurotrophic Factors and Their Receptors in Adult Zebrafish Spinal Cord Cacialli, Pietro Ricci, Serena Lazzari, Maurizio Milani, Liliana Franceschini, Valeria Int J Mol Sci Article In vertebrates, neurotrophins and their receptors play a fundamental role in the central and peripheral nervous systems. Several studies reported that each neurotrophin/receptor signalling pathway can perform various functions during axon development, neuronal growth, and plasticity. Previous investigations in some fish species have identified neurotrophins and their receptors in the spinal cord under physiological conditions and after injuries, highlighting their potential role during regeneration. In our study, for the first time, we used an excellent animal model, the zebrafish (Danio rerio), to compare the mRNA localization patterns of neurotrophins and receptors in the spinal cord. We quantified the levels of mRNA using qPCR, and identified the transcription pattern of each neurotrophin/receptor pathway via in situ hybridization. Our data show that ngf/trka are the most transcribed members in the adult zebrafish spinal cord. MDPI 2023-06-30 /pmc/articles/PMC10341495/ /pubmed/37446129 http://dx.doi.org/10.3390/ijms241310953 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Cacialli, Pietro Ricci, Serena Lazzari, Maurizio Milani, Liliana Franceschini, Valeria Transcription Pattern of Neurotrophic Factors and Their Receptors in Adult Zebrafish Spinal Cord |
title | Transcription Pattern of Neurotrophic Factors and Their Receptors in Adult Zebrafish Spinal Cord |
title_full | Transcription Pattern of Neurotrophic Factors and Their Receptors in Adult Zebrafish Spinal Cord |
title_fullStr | Transcription Pattern of Neurotrophic Factors and Their Receptors in Adult Zebrafish Spinal Cord |
title_full_unstemmed | Transcription Pattern of Neurotrophic Factors and Their Receptors in Adult Zebrafish Spinal Cord |
title_short | Transcription Pattern of Neurotrophic Factors and Their Receptors in Adult Zebrafish Spinal Cord |
title_sort | transcription pattern of neurotrophic factors and their receptors in adult zebrafish spinal cord |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10341495/ https://www.ncbi.nlm.nih.gov/pubmed/37446129 http://dx.doi.org/10.3390/ijms241310953 |
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