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Antibacterial Films of Silver Nanoparticles Embedded into Carboxymethylcellulose/Chitosan Multilayers on Nanoporous Silicon: A Layer-by-Layer Assembly Approach Comparing Dip and Spin Coating

The design and engineering of antibacterial materials are key for preventing bacterial adherence and proliferation in biomedical and household instruments. Silver nanoparticles (AgNPs) and chitosan (CHI) are broad-spectrum antibacterial materials with different properties whose combined application...

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Detalles Bibliográficos
Autores principales: Naveas, Nelson, Pulido, Ruth, Torres-Costa, Vicente, Agulló-Rueda, Fernando, Santibáñez, Mauricio, Malano, Francisco, Recio-Sánchez, Gonzalo, Garrido-Miranda, Karla A., Manso-Silván, Miguel, Hernández-Montelongo, Jacobo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10341592/
https://www.ncbi.nlm.nih.gov/pubmed/37445773
http://dx.doi.org/10.3390/ijms241310595
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author Naveas, Nelson
Pulido, Ruth
Torres-Costa, Vicente
Agulló-Rueda, Fernando
Santibáñez, Mauricio
Malano, Francisco
Recio-Sánchez, Gonzalo
Garrido-Miranda, Karla A.
Manso-Silván, Miguel
Hernández-Montelongo, Jacobo
author_facet Naveas, Nelson
Pulido, Ruth
Torres-Costa, Vicente
Agulló-Rueda, Fernando
Santibáñez, Mauricio
Malano, Francisco
Recio-Sánchez, Gonzalo
Garrido-Miranda, Karla A.
Manso-Silván, Miguel
Hernández-Montelongo, Jacobo
author_sort Naveas, Nelson
collection PubMed
description The design and engineering of antibacterial materials are key for preventing bacterial adherence and proliferation in biomedical and household instruments. Silver nanoparticles (AgNPs) and chitosan (CHI) are broad-spectrum antibacterial materials with different properties whose combined application is currently under optimization. This study proposes the formation of antibacterial films with AgNPs embedded in carboxymethylcellulose/chitosan multilayers by the layer-by-layer (LbL) method. The films were deposited onto nanoporous silicon (nPSi), an ideal platform for bioengineering applications due to its biocompatibility, biodegradability, and bioresorbability. We focused on two alternative multilayer deposition processes: cyclic dip coating (CDC) and cyclic spin coating (CSC). The physicochemical properties of the films were the subject of microscopic, microstructural, and surface–interface analyses. The antibacterial activity of each film was investigated against Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive) bacteria strains as model microorganisms. According to the findings, the CDC technique produced multilayer films with higher antibacterial activity for both bacteria compared to the CSC method. Bacteria adhesion inhibition was observed from only three cycles. The developed AgNPs–multilayer composite film offers advantageous antibacterial properties for biomedical applications.
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spelling pubmed-103415922023-07-14 Antibacterial Films of Silver Nanoparticles Embedded into Carboxymethylcellulose/Chitosan Multilayers on Nanoporous Silicon: A Layer-by-Layer Assembly Approach Comparing Dip and Spin Coating Naveas, Nelson Pulido, Ruth Torres-Costa, Vicente Agulló-Rueda, Fernando Santibáñez, Mauricio Malano, Francisco Recio-Sánchez, Gonzalo Garrido-Miranda, Karla A. Manso-Silván, Miguel Hernández-Montelongo, Jacobo Int J Mol Sci Article The design and engineering of antibacterial materials are key for preventing bacterial adherence and proliferation in biomedical and household instruments. Silver nanoparticles (AgNPs) and chitosan (CHI) are broad-spectrum antibacterial materials with different properties whose combined application is currently under optimization. This study proposes the formation of antibacterial films with AgNPs embedded in carboxymethylcellulose/chitosan multilayers by the layer-by-layer (LbL) method. The films were deposited onto nanoporous silicon (nPSi), an ideal platform for bioengineering applications due to its biocompatibility, biodegradability, and bioresorbability. We focused on two alternative multilayer deposition processes: cyclic dip coating (CDC) and cyclic spin coating (CSC). The physicochemical properties of the films were the subject of microscopic, microstructural, and surface–interface analyses. The antibacterial activity of each film was investigated against Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive) bacteria strains as model microorganisms. According to the findings, the CDC technique produced multilayer films with higher antibacterial activity for both bacteria compared to the CSC method. Bacteria adhesion inhibition was observed from only three cycles. The developed AgNPs–multilayer composite film offers advantageous antibacterial properties for biomedical applications. MDPI 2023-06-24 /pmc/articles/PMC10341592/ /pubmed/37445773 http://dx.doi.org/10.3390/ijms241310595 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Naveas, Nelson
Pulido, Ruth
Torres-Costa, Vicente
Agulló-Rueda, Fernando
Santibáñez, Mauricio
Malano, Francisco
Recio-Sánchez, Gonzalo
Garrido-Miranda, Karla A.
Manso-Silván, Miguel
Hernández-Montelongo, Jacobo
Antibacterial Films of Silver Nanoparticles Embedded into Carboxymethylcellulose/Chitosan Multilayers on Nanoporous Silicon: A Layer-by-Layer Assembly Approach Comparing Dip and Spin Coating
title Antibacterial Films of Silver Nanoparticles Embedded into Carboxymethylcellulose/Chitosan Multilayers on Nanoporous Silicon: A Layer-by-Layer Assembly Approach Comparing Dip and Spin Coating
title_full Antibacterial Films of Silver Nanoparticles Embedded into Carboxymethylcellulose/Chitosan Multilayers on Nanoporous Silicon: A Layer-by-Layer Assembly Approach Comparing Dip and Spin Coating
title_fullStr Antibacterial Films of Silver Nanoparticles Embedded into Carboxymethylcellulose/Chitosan Multilayers on Nanoporous Silicon: A Layer-by-Layer Assembly Approach Comparing Dip and Spin Coating
title_full_unstemmed Antibacterial Films of Silver Nanoparticles Embedded into Carboxymethylcellulose/Chitosan Multilayers on Nanoporous Silicon: A Layer-by-Layer Assembly Approach Comparing Dip and Spin Coating
title_short Antibacterial Films of Silver Nanoparticles Embedded into Carboxymethylcellulose/Chitosan Multilayers on Nanoporous Silicon: A Layer-by-Layer Assembly Approach Comparing Dip and Spin Coating
title_sort antibacterial films of silver nanoparticles embedded into carboxymethylcellulose/chitosan multilayers on nanoporous silicon: a layer-by-layer assembly approach comparing dip and spin coating
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10341592/
https://www.ncbi.nlm.nih.gov/pubmed/37445773
http://dx.doi.org/10.3390/ijms241310595
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