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Vaccine-Induced Immunity Elicited by Microneedle Delivery of Influenza Ectodomain Matrix Protein 2 Virus-like Particle (M2e VLP)-Loaded PLGA Nanoparticles

This study focused on developing an influenza vaccine delivered in polymeric nanoparticles (NPs) using dissolving microneedles. We first formulated an influenza extracellular matrix protein 2 virus-like particle (M2e VLP)-loaded with poly(lactic-co-glycolic) acid (PLGA) nanoparticles, yielding M2e5x...

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Autores principales: Braz Gomes, Keegan, Vijayanand, Sharon, Bagwe, Priyal, Menon, Ipshita, Kale, Akanksha, Patil, Smital, Kang, Sang-Moo, Uddin, Mohammad N., D’Souza, Martin J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10341628/
https://www.ncbi.nlm.nih.gov/pubmed/37445784
http://dx.doi.org/10.3390/ijms241310612
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author Braz Gomes, Keegan
Vijayanand, Sharon
Bagwe, Priyal
Menon, Ipshita
Kale, Akanksha
Patil, Smital
Kang, Sang-Moo
Uddin, Mohammad N.
D’Souza, Martin J.
author_facet Braz Gomes, Keegan
Vijayanand, Sharon
Bagwe, Priyal
Menon, Ipshita
Kale, Akanksha
Patil, Smital
Kang, Sang-Moo
Uddin, Mohammad N.
D’Souza, Martin J.
author_sort Braz Gomes, Keegan
collection PubMed
description This study focused on developing an influenza vaccine delivered in polymeric nanoparticles (NPs) using dissolving microneedles. We first formulated an influenza extracellular matrix protein 2 virus-like particle (M2e VLP)-loaded with poly(lactic-co-glycolic) acid (PLGA) nanoparticles, yielding M2e5x VLP PLGA NPs. The vaccine particles were characterized for their physical properties and in vitro immunogenicity. Next, the M2e5x VLP PLGA NPs, along with the adjuvant Alhydrogel(®) and monophosphoryl lipid A(®) (MPL-A(®)) PLGA NPs, were loaded into fast-dissolving microneedles. The vaccine microneedle patches were then evaluated in vivo in a murine model. The results from this study demonstrated that the vaccine nanoparticles effectively stimulated antigen-presenting cells in vitro resulting in enhanced autophagy, nitric oxide, and antigen presentation. In mice, the vaccine elicited M2e-specific antibodies in both serum and lung supernatants (post-challenge) and induced significant expression of CD4(+) and CD8(+) populations in the lymph nodes and spleens of immunized mice. Hence, this study demonstrated that polymeric particulates for antigen and adjuvant encapsulation, delivered using fast-dissolving microneedles, significantly enhanced the immunogenicity of a conserved influenza antigen.
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spelling pubmed-103416282023-07-14 Vaccine-Induced Immunity Elicited by Microneedle Delivery of Influenza Ectodomain Matrix Protein 2 Virus-like Particle (M2e VLP)-Loaded PLGA Nanoparticles Braz Gomes, Keegan Vijayanand, Sharon Bagwe, Priyal Menon, Ipshita Kale, Akanksha Patil, Smital Kang, Sang-Moo Uddin, Mohammad N. D’Souza, Martin J. Int J Mol Sci Article This study focused on developing an influenza vaccine delivered in polymeric nanoparticles (NPs) using dissolving microneedles. We first formulated an influenza extracellular matrix protein 2 virus-like particle (M2e VLP)-loaded with poly(lactic-co-glycolic) acid (PLGA) nanoparticles, yielding M2e5x VLP PLGA NPs. The vaccine particles were characterized for their physical properties and in vitro immunogenicity. Next, the M2e5x VLP PLGA NPs, along with the adjuvant Alhydrogel(®) and monophosphoryl lipid A(®) (MPL-A(®)) PLGA NPs, were loaded into fast-dissolving microneedles. The vaccine microneedle patches were then evaluated in vivo in a murine model. The results from this study demonstrated that the vaccine nanoparticles effectively stimulated antigen-presenting cells in vitro resulting in enhanced autophagy, nitric oxide, and antigen presentation. In mice, the vaccine elicited M2e-specific antibodies in both serum and lung supernatants (post-challenge) and induced significant expression of CD4(+) and CD8(+) populations in the lymph nodes and spleens of immunized mice. Hence, this study demonstrated that polymeric particulates for antigen and adjuvant encapsulation, delivered using fast-dissolving microneedles, significantly enhanced the immunogenicity of a conserved influenza antigen. MDPI 2023-06-25 /pmc/articles/PMC10341628/ /pubmed/37445784 http://dx.doi.org/10.3390/ijms241310612 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Braz Gomes, Keegan
Vijayanand, Sharon
Bagwe, Priyal
Menon, Ipshita
Kale, Akanksha
Patil, Smital
Kang, Sang-Moo
Uddin, Mohammad N.
D’Souza, Martin J.
Vaccine-Induced Immunity Elicited by Microneedle Delivery of Influenza Ectodomain Matrix Protein 2 Virus-like Particle (M2e VLP)-Loaded PLGA Nanoparticles
title Vaccine-Induced Immunity Elicited by Microneedle Delivery of Influenza Ectodomain Matrix Protein 2 Virus-like Particle (M2e VLP)-Loaded PLGA Nanoparticles
title_full Vaccine-Induced Immunity Elicited by Microneedle Delivery of Influenza Ectodomain Matrix Protein 2 Virus-like Particle (M2e VLP)-Loaded PLGA Nanoparticles
title_fullStr Vaccine-Induced Immunity Elicited by Microneedle Delivery of Influenza Ectodomain Matrix Protein 2 Virus-like Particle (M2e VLP)-Loaded PLGA Nanoparticles
title_full_unstemmed Vaccine-Induced Immunity Elicited by Microneedle Delivery of Influenza Ectodomain Matrix Protein 2 Virus-like Particle (M2e VLP)-Loaded PLGA Nanoparticles
title_short Vaccine-Induced Immunity Elicited by Microneedle Delivery of Influenza Ectodomain Matrix Protein 2 Virus-like Particle (M2e VLP)-Loaded PLGA Nanoparticles
title_sort vaccine-induced immunity elicited by microneedle delivery of influenza ectodomain matrix protein 2 virus-like particle (m2e vlp)-loaded plga nanoparticles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10341628/
https://www.ncbi.nlm.nih.gov/pubmed/37445784
http://dx.doi.org/10.3390/ijms241310612
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