Cargando…

LC-MS/MS-Based Serum Metabolomics and Transcriptome Analyses for the Mechanism of Augmented Renal Clearance

Augmented Renal Clearance (ARC) refers to the increased renal clearance of circulating solute in critically ill patients. In this study, the analytical research method of transcriptomics combined with metabolomics was used to study the pathogenesis of ARC at the transcriptional and metabolic levels....

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Yidan, Luo, Yifan, Yang, Shu, Jiang, Mingyan, Chu, Yang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10341629/
https://www.ncbi.nlm.nih.gov/pubmed/37445637
http://dx.doi.org/10.3390/ijms241310459
_version_ 1785072307248562176
author Wang, Yidan
Luo, Yifan
Yang, Shu
Jiang, Mingyan
Chu, Yang
author_facet Wang, Yidan
Luo, Yifan
Yang, Shu
Jiang, Mingyan
Chu, Yang
author_sort Wang, Yidan
collection PubMed
description Augmented Renal Clearance (ARC) refers to the increased renal clearance of circulating solute in critically ill patients. In this study, the analytical research method of transcriptomics combined with metabolomics was used to study the pathogenesis of ARC at the transcriptional and metabolic levels. In transcriptomics, 534 samples from 5 datasets in the Gene Expression Omnibus database were analyzed and 834 differential genes associated with ARC were obtained. In metabolomics, we used Ultra-Performance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry to determine the non-targeted metabolites of 102 samples after matching propensity scores, and obtained 45 differential metabolites associated with ARC. The results of the combined analysis showed that purine metabolism, arginine biosynthesis, and arachidonic acid metabolism were changed in patients with ARC. We speculate that the occurrence of ARC may be related to the alteration of renal blood perfusion by LTB4R, ARG1, ALOX5, arginine and prostaglandins E2 through inflammatory response, as well as the effects of CA4, PFKFB2, PFKFB3, PRKACB, NMDAR, glutamate and cAMP on renal capillary wall permeability.
format Online
Article
Text
id pubmed-10341629
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-103416292023-07-14 LC-MS/MS-Based Serum Metabolomics and Transcriptome Analyses for the Mechanism of Augmented Renal Clearance Wang, Yidan Luo, Yifan Yang, Shu Jiang, Mingyan Chu, Yang Int J Mol Sci Article Augmented Renal Clearance (ARC) refers to the increased renal clearance of circulating solute in critically ill patients. In this study, the analytical research method of transcriptomics combined with metabolomics was used to study the pathogenesis of ARC at the transcriptional and metabolic levels. In transcriptomics, 534 samples from 5 datasets in the Gene Expression Omnibus database were analyzed and 834 differential genes associated with ARC were obtained. In metabolomics, we used Ultra-Performance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry to determine the non-targeted metabolites of 102 samples after matching propensity scores, and obtained 45 differential metabolites associated with ARC. The results of the combined analysis showed that purine metabolism, arginine biosynthesis, and arachidonic acid metabolism were changed in patients with ARC. We speculate that the occurrence of ARC may be related to the alteration of renal blood perfusion by LTB4R, ARG1, ALOX5, arginine and prostaglandins E2 through inflammatory response, as well as the effects of CA4, PFKFB2, PFKFB3, PRKACB, NMDAR, glutamate and cAMP on renal capillary wall permeability. MDPI 2023-06-21 /pmc/articles/PMC10341629/ /pubmed/37445637 http://dx.doi.org/10.3390/ijms241310459 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Yidan
Luo, Yifan
Yang, Shu
Jiang, Mingyan
Chu, Yang
LC-MS/MS-Based Serum Metabolomics and Transcriptome Analyses for the Mechanism of Augmented Renal Clearance
title LC-MS/MS-Based Serum Metabolomics and Transcriptome Analyses for the Mechanism of Augmented Renal Clearance
title_full LC-MS/MS-Based Serum Metabolomics and Transcriptome Analyses for the Mechanism of Augmented Renal Clearance
title_fullStr LC-MS/MS-Based Serum Metabolomics and Transcriptome Analyses for the Mechanism of Augmented Renal Clearance
title_full_unstemmed LC-MS/MS-Based Serum Metabolomics and Transcriptome Analyses for the Mechanism of Augmented Renal Clearance
title_short LC-MS/MS-Based Serum Metabolomics and Transcriptome Analyses for the Mechanism of Augmented Renal Clearance
title_sort lc-ms/ms-based serum metabolomics and transcriptome analyses for the mechanism of augmented renal clearance
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10341629/
https://www.ncbi.nlm.nih.gov/pubmed/37445637
http://dx.doi.org/10.3390/ijms241310459
work_keys_str_mv AT wangyidan lcmsmsbasedserummetabolomicsandtranscriptomeanalysesforthemechanismofaugmentedrenalclearance
AT luoyifan lcmsmsbasedserummetabolomicsandtranscriptomeanalysesforthemechanismofaugmentedrenalclearance
AT yangshu lcmsmsbasedserummetabolomicsandtranscriptomeanalysesforthemechanismofaugmentedrenalclearance
AT jiangmingyan lcmsmsbasedserummetabolomicsandtranscriptomeanalysesforthemechanismofaugmentedrenalclearance
AT chuyang lcmsmsbasedserummetabolomicsandtranscriptomeanalysesforthemechanismofaugmentedrenalclearance