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Genome-Wide Analysis of the MADS-box Gene Family and Expression Analysis during Anther Development in Salvia miltiorrhiza

MADS-box genes constitute a large family of transcription factors that play important roles in plant growth and development. However, our understanding of MADS-box genes involved in anther development and male sterility in Salvia miltiorrhiza is still limited. In this study, 63 MADS-box genes were i...

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Autores principales: Chai, Songyue, Li, Kexin, Deng, Xuexue, Wang, Long, Jiang, Yuanyuan, Liao, Jinqiu, Yang, Ruiwu, Zhang, Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10341755/
https://www.ncbi.nlm.nih.gov/pubmed/37446115
http://dx.doi.org/10.3390/ijms241310937
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author Chai, Songyue
Li, Kexin
Deng, Xuexue
Wang, Long
Jiang, Yuanyuan
Liao, Jinqiu
Yang, Ruiwu
Zhang, Li
author_facet Chai, Songyue
Li, Kexin
Deng, Xuexue
Wang, Long
Jiang, Yuanyuan
Liao, Jinqiu
Yang, Ruiwu
Zhang, Li
author_sort Chai, Songyue
collection PubMed
description MADS-box genes constitute a large family of transcription factors that play important roles in plant growth and development. However, our understanding of MADS-box genes involved in anther development and male sterility in Salvia miltiorrhiza is still limited. In this study, 63 MADS-box genes were identified from the genome of the male sterility ecotype Sichuan S. miltiorrhiza (S. miltiorrhiza_SC) unevenly distributed among eight chromosomes. Phylogenetic analysis classified them into two types and 17 subfamilies. They contained 1 to 12 exons and 10 conserved motifs. Evolution analysis showed that segmental duplication was the main force for the expansion of the SmMADS gene family, and duplication gene pairs were under purifying selection. Cis-acting elements analysis demonstrated that the promoter of SmMADS genes contain numerous elements associated with plant growth and development, plant hormones, and stress response. RNA-seq showed that the expression levels of B-class and C-class SmMADS genes were highly expressed during anther development, with SmMADS11 likely playing an important role in regulating anther development and male fertility in S. miltiorrhiza_SC. Overall, this study provides a comprehensive analysis of the MADS-box gene family in S. miltiorrhiza, shedding light on their potential role in anther development and male sterility.
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spelling pubmed-103417552023-07-14 Genome-Wide Analysis of the MADS-box Gene Family and Expression Analysis during Anther Development in Salvia miltiorrhiza Chai, Songyue Li, Kexin Deng, Xuexue Wang, Long Jiang, Yuanyuan Liao, Jinqiu Yang, Ruiwu Zhang, Li Int J Mol Sci Article MADS-box genes constitute a large family of transcription factors that play important roles in plant growth and development. However, our understanding of MADS-box genes involved in anther development and male sterility in Salvia miltiorrhiza is still limited. In this study, 63 MADS-box genes were identified from the genome of the male sterility ecotype Sichuan S. miltiorrhiza (S. miltiorrhiza_SC) unevenly distributed among eight chromosomes. Phylogenetic analysis classified them into two types and 17 subfamilies. They contained 1 to 12 exons and 10 conserved motifs. Evolution analysis showed that segmental duplication was the main force for the expansion of the SmMADS gene family, and duplication gene pairs were under purifying selection. Cis-acting elements analysis demonstrated that the promoter of SmMADS genes contain numerous elements associated with plant growth and development, plant hormones, and stress response. RNA-seq showed that the expression levels of B-class and C-class SmMADS genes were highly expressed during anther development, with SmMADS11 likely playing an important role in regulating anther development and male fertility in S. miltiorrhiza_SC. Overall, this study provides a comprehensive analysis of the MADS-box gene family in S. miltiorrhiza, shedding light on their potential role in anther development and male sterility. MDPI 2023-06-30 /pmc/articles/PMC10341755/ /pubmed/37446115 http://dx.doi.org/10.3390/ijms241310937 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chai, Songyue
Li, Kexin
Deng, Xuexue
Wang, Long
Jiang, Yuanyuan
Liao, Jinqiu
Yang, Ruiwu
Zhang, Li
Genome-Wide Analysis of the MADS-box Gene Family and Expression Analysis during Anther Development in Salvia miltiorrhiza
title Genome-Wide Analysis of the MADS-box Gene Family and Expression Analysis during Anther Development in Salvia miltiorrhiza
title_full Genome-Wide Analysis of the MADS-box Gene Family and Expression Analysis during Anther Development in Salvia miltiorrhiza
title_fullStr Genome-Wide Analysis of the MADS-box Gene Family and Expression Analysis during Anther Development in Salvia miltiorrhiza
title_full_unstemmed Genome-Wide Analysis of the MADS-box Gene Family and Expression Analysis during Anther Development in Salvia miltiorrhiza
title_short Genome-Wide Analysis of the MADS-box Gene Family and Expression Analysis during Anther Development in Salvia miltiorrhiza
title_sort genome-wide analysis of the mads-box gene family and expression analysis during anther development in salvia miltiorrhiza
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10341755/
https://www.ncbi.nlm.nih.gov/pubmed/37446115
http://dx.doi.org/10.3390/ijms241310937
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