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Discovery of Novel Pentacyclic Triterpene Acid Amide Derivatives as Excellent Antimicrobial Agents Dependent on Generation of Reactive Oxygen Species

Developing new agricultural bactericides is a feasible strategy for stopping the increase in the resistance of plant pathogenic bacteria. Some pentacyclic triterpene acid derivatives were elaborately designed and synthesized. In particular, compound A(22) exhibited the best antimicrobial activity ag...

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Detalles Bibliográficos
Autores principales: Yang, Yihong, Chen, Kunlun, Wang, Guangdi, Liu, Hongwu, Shao, Lihui, Zhou, Xiang, Liu, Liwei, Yang, Song
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10341837/
https://www.ncbi.nlm.nih.gov/pubmed/37445744
http://dx.doi.org/10.3390/ijms241310566
Descripción
Sumario:Developing new agricultural bactericides is a feasible strategy for stopping the increase in the resistance of plant pathogenic bacteria. Some pentacyclic triterpene acid derivatives were elaborately designed and synthesized. In particular, compound A(22) exhibited the best antimicrobial activity against Xanthomonas oryzae pv. oryzae (Xoo) and Xanthomonas axonopodis pv. citri (Xac) with EC(50) values of 3.34 and 3.30 mg L(−1), respectively. The antimicrobial mechanism showed that the compound A(22) induced excessive production and accumulation of reactive oxygen species (ROS) in Xoo cells, leading to a decrease in superoxide dismutase and catalase enzyme activities and an increase in malondialdehyde content. A(22) also produced increases in Xoo cell membrane permeability and eventual cell death. In addition, in vivo experiments showed that A(22) at 200 mg L(−1) exhibited protective activity against rice bacterial blight (50.44%) and citrus canker disease (84.37%). Therefore, this study provides a paradigm for the agricultural application of pentacyclic triterpene acid.