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Discovery of Novel Pentacyclic Triterpene Acid Amide Derivatives as Excellent Antimicrobial Agents Dependent on Generation of Reactive Oxygen Species
Developing new agricultural bactericides is a feasible strategy for stopping the increase in the resistance of plant pathogenic bacteria. Some pentacyclic triterpene acid derivatives were elaborately designed and synthesized. In particular, compound A(22) exhibited the best antimicrobial activity ag...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10341837/ https://www.ncbi.nlm.nih.gov/pubmed/37445744 http://dx.doi.org/10.3390/ijms241310566 |
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author | Yang, Yihong Chen, Kunlun Wang, Guangdi Liu, Hongwu Shao, Lihui Zhou, Xiang Liu, Liwei Yang, Song |
author_facet | Yang, Yihong Chen, Kunlun Wang, Guangdi Liu, Hongwu Shao, Lihui Zhou, Xiang Liu, Liwei Yang, Song |
author_sort | Yang, Yihong |
collection | PubMed |
description | Developing new agricultural bactericides is a feasible strategy for stopping the increase in the resistance of plant pathogenic bacteria. Some pentacyclic triterpene acid derivatives were elaborately designed and synthesized. In particular, compound A(22) exhibited the best antimicrobial activity against Xanthomonas oryzae pv. oryzae (Xoo) and Xanthomonas axonopodis pv. citri (Xac) with EC(50) values of 3.34 and 3.30 mg L(−1), respectively. The antimicrobial mechanism showed that the compound A(22) induced excessive production and accumulation of reactive oxygen species (ROS) in Xoo cells, leading to a decrease in superoxide dismutase and catalase enzyme activities and an increase in malondialdehyde content. A(22) also produced increases in Xoo cell membrane permeability and eventual cell death. In addition, in vivo experiments showed that A(22) at 200 mg L(−1) exhibited protective activity against rice bacterial blight (50.44%) and citrus canker disease (84.37%). Therefore, this study provides a paradigm for the agricultural application of pentacyclic triterpene acid. |
format | Online Article Text |
id | pubmed-10341837 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-103418372023-07-14 Discovery of Novel Pentacyclic Triterpene Acid Amide Derivatives as Excellent Antimicrobial Agents Dependent on Generation of Reactive Oxygen Species Yang, Yihong Chen, Kunlun Wang, Guangdi Liu, Hongwu Shao, Lihui Zhou, Xiang Liu, Liwei Yang, Song Int J Mol Sci Article Developing new agricultural bactericides is a feasible strategy for stopping the increase in the resistance of plant pathogenic bacteria. Some pentacyclic triterpene acid derivatives were elaborately designed and synthesized. In particular, compound A(22) exhibited the best antimicrobial activity against Xanthomonas oryzae pv. oryzae (Xoo) and Xanthomonas axonopodis pv. citri (Xac) with EC(50) values of 3.34 and 3.30 mg L(−1), respectively. The antimicrobial mechanism showed that the compound A(22) induced excessive production and accumulation of reactive oxygen species (ROS) in Xoo cells, leading to a decrease in superoxide dismutase and catalase enzyme activities and an increase in malondialdehyde content. A(22) also produced increases in Xoo cell membrane permeability and eventual cell death. In addition, in vivo experiments showed that A(22) at 200 mg L(−1) exhibited protective activity against rice bacterial blight (50.44%) and citrus canker disease (84.37%). Therefore, this study provides a paradigm for the agricultural application of pentacyclic triterpene acid. MDPI 2023-06-24 /pmc/articles/PMC10341837/ /pubmed/37445744 http://dx.doi.org/10.3390/ijms241310566 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yang, Yihong Chen, Kunlun Wang, Guangdi Liu, Hongwu Shao, Lihui Zhou, Xiang Liu, Liwei Yang, Song Discovery of Novel Pentacyclic Triterpene Acid Amide Derivatives as Excellent Antimicrobial Agents Dependent on Generation of Reactive Oxygen Species |
title | Discovery of Novel Pentacyclic Triterpene Acid Amide Derivatives as Excellent Antimicrobial Agents Dependent on Generation of Reactive Oxygen Species |
title_full | Discovery of Novel Pentacyclic Triterpene Acid Amide Derivatives as Excellent Antimicrobial Agents Dependent on Generation of Reactive Oxygen Species |
title_fullStr | Discovery of Novel Pentacyclic Triterpene Acid Amide Derivatives as Excellent Antimicrobial Agents Dependent on Generation of Reactive Oxygen Species |
title_full_unstemmed | Discovery of Novel Pentacyclic Triterpene Acid Amide Derivatives as Excellent Antimicrobial Agents Dependent on Generation of Reactive Oxygen Species |
title_short | Discovery of Novel Pentacyclic Triterpene Acid Amide Derivatives as Excellent Antimicrobial Agents Dependent on Generation of Reactive Oxygen Species |
title_sort | discovery of novel pentacyclic triterpene acid amide derivatives as excellent antimicrobial agents dependent on generation of reactive oxygen species |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10341837/ https://www.ncbi.nlm.nih.gov/pubmed/37445744 http://dx.doi.org/10.3390/ijms241310566 |
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