Cargando…
Effects of Individual Circulating FFAs on Plasma and Hepatic FFA Epoxides, Diols, and Epoxide-Diol Ratios as Indices of Soluble Epoxide Hydrolase Activity
Oxylipins, oxidation products of unsaturated free fatty acids (FFAs), are involved in various cellular signaling systems. Among these oxylipins, FFA epoxides are associated with beneficial effects in metabolic and cardiovascular health. FFA epoxides are metabolized to diols, which are usually biolog...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10341844/ https://www.ncbi.nlm.nih.gov/pubmed/37445935 http://dx.doi.org/10.3390/ijms241310760 |
_version_ | 1785072358515539968 |
---|---|
author | Oh, Young Taek Yang, Jun Morisseau, Christophe He, Qiyi Hammock, Bruce Youn, Jang H. |
author_facet | Oh, Young Taek Yang, Jun Morisseau, Christophe He, Qiyi Hammock, Bruce Youn, Jang H. |
author_sort | Oh, Young Taek |
collection | PubMed |
description | Oxylipins, oxidation products of unsaturated free fatty acids (FFAs), are involved in various cellular signaling systems. Among these oxylipins, FFA epoxides are associated with beneficial effects in metabolic and cardiovascular health. FFA epoxides are metabolized to diols, which are usually biologically less active, by soluble epoxide hydrolase (sEH). Plasma epoxide-diol ratios have been used as indirect measures of sEH activity. This study was designed to examine the effects of acute elevation of individual plasma FFAs on a variety of oxylipins, particularly epoxides, diols, and their ratios. We tested if FFA epoxide-diol ratios are altered by circulating FFA levels (i.e., substrate availability) independent of sEH activity. Wistar rats received a constant intravenous infusion of olive (70% oleic acid (OA)), safflower seed (72% linoleic acid (LA)), and fish oils (rich in ω-3 FFAs) as emulsions to selectively raise OA, LA, and ω-3 FFAs (eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)), respectively. As expected, olive, safflower seed, and fish oil infusions selectively raised plasma OA (57%), LA (87%), EPA (70%), and DHA (54%), respectively (p < 0.05 for all). Raising plasma FFAs exerted substrate effects to increase hepatic and plasma epoxide and diol levels. These increases in epoxides and diols occurred to similar extents, resulting in no significant changes in epoxide-diol ratios. These data suggest that epoxide-diol ratios, often used as indices of sEH activity, are not affected by substrate availability or altered plasma FFA levels and that epoxide-diol ratios may be used to compare sEH activity between conditions of different circulating FFA levels. |
format | Online Article Text |
id | pubmed-10341844 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-103418442023-07-14 Effects of Individual Circulating FFAs on Plasma and Hepatic FFA Epoxides, Diols, and Epoxide-Diol Ratios as Indices of Soluble Epoxide Hydrolase Activity Oh, Young Taek Yang, Jun Morisseau, Christophe He, Qiyi Hammock, Bruce Youn, Jang H. Int J Mol Sci Article Oxylipins, oxidation products of unsaturated free fatty acids (FFAs), are involved in various cellular signaling systems. Among these oxylipins, FFA epoxides are associated with beneficial effects in metabolic and cardiovascular health. FFA epoxides are metabolized to diols, which are usually biologically less active, by soluble epoxide hydrolase (sEH). Plasma epoxide-diol ratios have been used as indirect measures of sEH activity. This study was designed to examine the effects of acute elevation of individual plasma FFAs on a variety of oxylipins, particularly epoxides, diols, and their ratios. We tested if FFA epoxide-diol ratios are altered by circulating FFA levels (i.e., substrate availability) independent of sEH activity. Wistar rats received a constant intravenous infusion of olive (70% oleic acid (OA)), safflower seed (72% linoleic acid (LA)), and fish oils (rich in ω-3 FFAs) as emulsions to selectively raise OA, LA, and ω-3 FFAs (eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)), respectively. As expected, olive, safflower seed, and fish oil infusions selectively raised plasma OA (57%), LA (87%), EPA (70%), and DHA (54%), respectively (p < 0.05 for all). Raising plasma FFAs exerted substrate effects to increase hepatic and plasma epoxide and diol levels. These increases in epoxides and diols occurred to similar extents, resulting in no significant changes in epoxide-diol ratios. These data suggest that epoxide-diol ratios, often used as indices of sEH activity, are not affected by substrate availability or altered plasma FFA levels and that epoxide-diol ratios may be used to compare sEH activity between conditions of different circulating FFA levels. MDPI 2023-06-28 /pmc/articles/PMC10341844/ /pubmed/37445935 http://dx.doi.org/10.3390/ijms241310760 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Oh, Young Taek Yang, Jun Morisseau, Christophe He, Qiyi Hammock, Bruce Youn, Jang H. Effects of Individual Circulating FFAs on Plasma and Hepatic FFA Epoxides, Diols, and Epoxide-Diol Ratios as Indices of Soluble Epoxide Hydrolase Activity |
title | Effects of Individual Circulating FFAs on Plasma and Hepatic FFA Epoxides, Diols, and Epoxide-Diol Ratios as Indices of Soluble Epoxide Hydrolase Activity |
title_full | Effects of Individual Circulating FFAs on Plasma and Hepatic FFA Epoxides, Diols, and Epoxide-Diol Ratios as Indices of Soluble Epoxide Hydrolase Activity |
title_fullStr | Effects of Individual Circulating FFAs on Plasma and Hepatic FFA Epoxides, Diols, and Epoxide-Diol Ratios as Indices of Soluble Epoxide Hydrolase Activity |
title_full_unstemmed | Effects of Individual Circulating FFAs on Plasma and Hepatic FFA Epoxides, Diols, and Epoxide-Diol Ratios as Indices of Soluble Epoxide Hydrolase Activity |
title_short | Effects of Individual Circulating FFAs on Plasma and Hepatic FFA Epoxides, Diols, and Epoxide-Diol Ratios as Indices of Soluble Epoxide Hydrolase Activity |
title_sort | effects of individual circulating ffas on plasma and hepatic ffa epoxides, diols, and epoxide-diol ratios as indices of soluble epoxide hydrolase activity |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10341844/ https://www.ncbi.nlm.nih.gov/pubmed/37445935 http://dx.doi.org/10.3390/ijms241310760 |
work_keys_str_mv | AT ohyoungtaek effectsofindividualcirculatingffasonplasmaandhepaticffaepoxidesdiolsandepoxidediolratiosasindicesofsolubleepoxidehydrolaseactivity AT yangjun effectsofindividualcirculatingffasonplasmaandhepaticffaepoxidesdiolsandepoxidediolratiosasindicesofsolubleepoxidehydrolaseactivity AT morisseauchristophe effectsofindividualcirculatingffasonplasmaandhepaticffaepoxidesdiolsandepoxidediolratiosasindicesofsolubleepoxidehydrolaseactivity AT heqiyi effectsofindividualcirculatingffasonplasmaandhepaticffaepoxidesdiolsandepoxidediolratiosasindicesofsolubleepoxidehydrolaseactivity AT hammockbruce effectsofindividualcirculatingffasonplasmaandhepaticffaepoxidesdiolsandepoxidediolratiosasindicesofsolubleepoxidehydrolaseactivity AT younjangh effectsofindividualcirculatingffasonplasmaandhepaticffaepoxidesdiolsandepoxidediolratiosasindicesofsolubleepoxidehydrolaseactivity |