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Anti-Fibrotic Effects of RF Electric Currents

Hypertrophic scars and keloids are two different manifestations of excessive dermal fibrosis and are caused by an alteration in the normal wound-healing process. Treatment with radiofrequency (RF)-based therapies has proven to be useful in reducing hypertrophic scars. In this study, the effect of on...

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Autores principales: Hernández-Bule, María Luisa, Toledano-Macías, Elena, Pérez-González, Luis Alfonso, Martínez-Pascual, María Antonia, Fernández-Guarino, Montserrat
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10341950/
https://www.ncbi.nlm.nih.gov/pubmed/37446165
http://dx.doi.org/10.3390/ijms241310986
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author Hernández-Bule, María Luisa
Toledano-Macías, Elena
Pérez-González, Luis Alfonso
Martínez-Pascual, María Antonia
Fernández-Guarino, Montserrat
author_facet Hernández-Bule, María Luisa
Toledano-Macías, Elena
Pérez-González, Luis Alfonso
Martínez-Pascual, María Antonia
Fernández-Guarino, Montserrat
author_sort Hernández-Bule, María Luisa
collection PubMed
description Hypertrophic scars and keloids are two different manifestations of excessive dermal fibrosis and are caused by an alteration in the normal wound-healing process. Treatment with radiofrequency (RF)-based therapies has proven to be useful in reducing hypertrophic scars. In this study, the effect of one of these radiofrequency therapies, Capacitive Resistive Electrical Transfer Therapy (CRET) on biomarkers of skin fibrosis was investigated. For this, in cultures of human myofibroblasts treated with CRET therapy or sham-treated, proliferation (XTT Assay), apoptosis (TUNEL Assay), and cell migration (Wound Closure Assay) were analyzed. Furthermore, in these cultures the expression and/or localization of extracellular matrix proteins such as α-SMA, Col I, Col III (immunofluorescence), metalloproteinases MMP1 and MMP9, MAP kinase ERK1/2, and the transcription factor NFκB were also investigated (immunoblot). The results have revealed that CRET decreases the expression of extracellular matrix proteins, modifies the expression of the metalloproteinase MMP9, and reduces the activation of NFκB with respect to controls, suggesting that this therapy could be useful for the treatment of fibrotic pathologies.
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spelling pubmed-103419502023-07-14 Anti-Fibrotic Effects of RF Electric Currents Hernández-Bule, María Luisa Toledano-Macías, Elena Pérez-González, Luis Alfonso Martínez-Pascual, María Antonia Fernández-Guarino, Montserrat Int J Mol Sci Article Hypertrophic scars and keloids are two different manifestations of excessive dermal fibrosis and are caused by an alteration in the normal wound-healing process. Treatment with radiofrequency (RF)-based therapies has proven to be useful in reducing hypertrophic scars. In this study, the effect of one of these radiofrequency therapies, Capacitive Resistive Electrical Transfer Therapy (CRET) on biomarkers of skin fibrosis was investigated. For this, in cultures of human myofibroblasts treated with CRET therapy or sham-treated, proliferation (XTT Assay), apoptosis (TUNEL Assay), and cell migration (Wound Closure Assay) were analyzed. Furthermore, in these cultures the expression and/or localization of extracellular matrix proteins such as α-SMA, Col I, Col III (immunofluorescence), metalloproteinases MMP1 and MMP9, MAP kinase ERK1/2, and the transcription factor NFκB were also investigated (immunoblot). The results have revealed that CRET decreases the expression of extracellular matrix proteins, modifies the expression of the metalloproteinase MMP9, and reduces the activation of NFκB with respect to controls, suggesting that this therapy could be useful for the treatment of fibrotic pathologies. MDPI 2023-07-01 /pmc/articles/PMC10341950/ /pubmed/37446165 http://dx.doi.org/10.3390/ijms241310986 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hernández-Bule, María Luisa
Toledano-Macías, Elena
Pérez-González, Luis Alfonso
Martínez-Pascual, María Antonia
Fernández-Guarino, Montserrat
Anti-Fibrotic Effects of RF Electric Currents
title Anti-Fibrotic Effects of RF Electric Currents
title_full Anti-Fibrotic Effects of RF Electric Currents
title_fullStr Anti-Fibrotic Effects of RF Electric Currents
title_full_unstemmed Anti-Fibrotic Effects of RF Electric Currents
title_short Anti-Fibrotic Effects of RF Electric Currents
title_sort anti-fibrotic effects of rf electric currents
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10341950/
https://www.ncbi.nlm.nih.gov/pubmed/37446165
http://dx.doi.org/10.3390/ijms241310986
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