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Deciphering Molecular Mechanisms Governing the Reproductive Molt of Macrobrachium nipponense: A Transcriptome Analysis of Ovaries across Various Molting Stages

The relationship between molting and reproduction has received more attention in economically important crustacean decapods. Molting and reproduction are synergistic events in Macrobrachium nipponense, but the molecular regulatory mechanisms behind them are unclear. In the current study, we performe...

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Autores principales: Yuan, Huwei, Gao, Zijian, Cai, Pengfei, Zhang, Wenyi, Jin, Shubo, Jiang, Sufei, Xiong, Yiwei, Gong, Yongsheng, Qiao, Hui, Fu, Hongtuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10341968/
https://www.ncbi.nlm.nih.gov/pubmed/37446235
http://dx.doi.org/10.3390/ijms241311056
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author Yuan, Huwei
Gao, Zijian
Cai, Pengfei
Zhang, Wenyi
Jin, Shubo
Jiang, Sufei
Xiong, Yiwei
Gong, Yongsheng
Qiao, Hui
Fu, Hongtuo
author_facet Yuan, Huwei
Gao, Zijian
Cai, Pengfei
Zhang, Wenyi
Jin, Shubo
Jiang, Sufei
Xiong, Yiwei
Gong, Yongsheng
Qiao, Hui
Fu, Hongtuo
author_sort Yuan, Huwei
collection PubMed
description The relationship between molting and reproduction has received more attention in economically important crustacean decapods. Molting and reproduction are synergistic events in Macrobrachium nipponense, but the molecular regulatory mechanisms behind them are unclear. In the current study, we performed Illumina sequencing for the ovaries of M. nipponense during the molt cycle (pre-molting, Prm; mid-molting, Mm; and post-molting, Pom). A total of 66.57 Gb of transcriptome data were generated through sequencing, resulting in the identification of 105,149 unigenes whose alignment ratio with the reference genome exceeded 87.57%. Differentially expressed genes (DEGs) were annotated through the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases for gene classification and pathway analysis. A total of twenty-six molt-related DEGs were found, and their expression patterns were examined across various molting stages. The KEGG enrichment analysis revealed that the key pathways involved in regulating the molting process of M. nipponense primarily include the mTOR, insect hormone biosynthesis, TGF-beta, and Wnt signaling pathways. Our transcriptomic data suggest that these pathways crosstalk with each other to regulate the synthesis and degradation of ecdysone throughout the molt cycle. The current study has deepened our understanding of the molecular mechanisms of crustacean molting and will serve as a basis for future studies of crustaceans and other molting animals.
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spelling pubmed-103419682023-07-14 Deciphering Molecular Mechanisms Governing the Reproductive Molt of Macrobrachium nipponense: A Transcriptome Analysis of Ovaries across Various Molting Stages Yuan, Huwei Gao, Zijian Cai, Pengfei Zhang, Wenyi Jin, Shubo Jiang, Sufei Xiong, Yiwei Gong, Yongsheng Qiao, Hui Fu, Hongtuo Int J Mol Sci Article The relationship between molting and reproduction has received more attention in economically important crustacean decapods. Molting and reproduction are synergistic events in Macrobrachium nipponense, but the molecular regulatory mechanisms behind them are unclear. In the current study, we performed Illumina sequencing for the ovaries of M. nipponense during the molt cycle (pre-molting, Prm; mid-molting, Mm; and post-molting, Pom). A total of 66.57 Gb of transcriptome data were generated through sequencing, resulting in the identification of 105,149 unigenes whose alignment ratio with the reference genome exceeded 87.57%. Differentially expressed genes (DEGs) were annotated through the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases for gene classification and pathway analysis. A total of twenty-six molt-related DEGs were found, and their expression patterns were examined across various molting stages. The KEGG enrichment analysis revealed that the key pathways involved in regulating the molting process of M. nipponense primarily include the mTOR, insect hormone biosynthesis, TGF-beta, and Wnt signaling pathways. Our transcriptomic data suggest that these pathways crosstalk with each other to regulate the synthesis and degradation of ecdysone throughout the molt cycle. The current study has deepened our understanding of the molecular mechanisms of crustacean molting and will serve as a basis for future studies of crustaceans and other molting animals. MDPI 2023-07-04 /pmc/articles/PMC10341968/ /pubmed/37446235 http://dx.doi.org/10.3390/ijms241311056 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yuan, Huwei
Gao, Zijian
Cai, Pengfei
Zhang, Wenyi
Jin, Shubo
Jiang, Sufei
Xiong, Yiwei
Gong, Yongsheng
Qiao, Hui
Fu, Hongtuo
Deciphering Molecular Mechanisms Governing the Reproductive Molt of Macrobrachium nipponense: A Transcriptome Analysis of Ovaries across Various Molting Stages
title Deciphering Molecular Mechanisms Governing the Reproductive Molt of Macrobrachium nipponense: A Transcriptome Analysis of Ovaries across Various Molting Stages
title_full Deciphering Molecular Mechanisms Governing the Reproductive Molt of Macrobrachium nipponense: A Transcriptome Analysis of Ovaries across Various Molting Stages
title_fullStr Deciphering Molecular Mechanisms Governing the Reproductive Molt of Macrobrachium nipponense: A Transcriptome Analysis of Ovaries across Various Molting Stages
title_full_unstemmed Deciphering Molecular Mechanisms Governing the Reproductive Molt of Macrobrachium nipponense: A Transcriptome Analysis of Ovaries across Various Molting Stages
title_short Deciphering Molecular Mechanisms Governing the Reproductive Molt of Macrobrachium nipponense: A Transcriptome Analysis of Ovaries across Various Molting Stages
title_sort deciphering molecular mechanisms governing the reproductive molt of macrobrachium nipponense: a transcriptome analysis of ovaries across various molting stages
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10341968/
https://www.ncbi.nlm.nih.gov/pubmed/37446235
http://dx.doi.org/10.3390/ijms241311056
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