Cargando…

Differential Pattern of Cell Death and ROS Production in Human Airway Epithelial Cells Exposed to Quinones Combined with Heated-PM2.5 and/or Asian Sand Dust

The combined toxicological effects of airborne particulate matter (PM), such as PM2.5, and Asian sand dust (ASD), with surrounding chemicals, particularly quinones, on human airway epithelial cells remain underexplored. In this study, we established an in vitro combination exposure model using 1,2-n...

Descripción completa

Detalles Bibliográficos
Autores principales: Honda, Akiko, Inoue, Ken-ichiro, Higashihara, Makoto, Ichinose, Takamichi, Ueda, Kayo, Takano, Hirohisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10341977/
https://www.ncbi.nlm.nih.gov/pubmed/37445720
http://dx.doi.org/10.3390/ijms241310544
_version_ 1785072390467747840
author Honda, Akiko
Inoue, Ken-ichiro
Higashihara, Makoto
Ichinose, Takamichi
Ueda, Kayo
Takano, Hirohisa
author_facet Honda, Akiko
Inoue, Ken-ichiro
Higashihara, Makoto
Ichinose, Takamichi
Ueda, Kayo
Takano, Hirohisa
author_sort Honda, Akiko
collection PubMed
description The combined toxicological effects of airborne particulate matter (PM), such as PM2.5, and Asian sand dust (ASD), with surrounding chemicals, particularly quinones, on human airway epithelial cells remain underexplored. In this study, we established an in vitro combination exposure model using 1,2-naphthoquinones (NQ) and 9,10-phenanthroquinones (PQ) along with heated PM (h-PM2.5 and h-ASD) to investigate their potential synergistic effects. The impacts of quinones and heated PM on tetrazolium dye (WST-1) reduction, cell death, and cytokine and reactive oxygen species (ROS) production were examined. Results revealed that exposure to 9,10-PQ with h-PM2.5 and/or h-ASD dose-dependently increased WST-1 reduction at 1 μM compared to the corresponding control while markedly decreasing it at 10 μM. Higher early apoptotic, late apoptotic, or necrotic cell numbers were detected in 9,10-PQ + h-PM2.5 exposure than in 9,10-PQ + h-ASD or 9,10-PQ + h-PM2.5 + h-ASD. Additionally, 1,2-NQ + h-PM2.5 exposure also resulted in an increase in cell death compared to 1,2-NQ + h-ASD and 1,2-NQ + h-PM2.5 + h-ASD. Quinones with or without h-PM2.5, h-ASD, or h-PM2.5 + h-ASD significantly increased ROS production, especially with h-PM2.5. Our findings suggest that quinones, at relatively low concentrations, induce cell death synergistically in the presence of h-PM2.5 rather than h-ASD and h-PM2.5 + h-ASD, partially through the induction of apoptosis with increased ROS generation.
format Online
Article
Text
id pubmed-10341977
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-103419772023-07-14 Differential Pattern of Cell Death and ROS Production in Human Airway Epithelial Cells Exposed to Quinones Combined with Heated-PM2.5 and/or Asian Sand Dust Honda, Akiko Inoue, Ken-ichiro Higashihara, Makoto Ichinose, Takamichi Ueda, Kayo Takano, Hirohisa Int J Mol Sci Article The combined toxicological effects of airborne particulate matter (PM), such as PM2.5, and Asian sand dust (ASD), with surrounding chemicals, particularly quinones, on human airway epithelial cells remain underexplored. In this study, we established an in vitro combination exposure model using 1,2-naphthoquinones (NQ) and 9,10-phenanthroquinones (PQ) along with heated PM (h-PM2.5 and h-ASD) to investigate their potential synergistic effects. The impacts of quinones and heated PM on tetrazolium dye (WST-1) reduction, cell death, and cytokine and reactive oxygen species (ROS) production were examined. Results revealed that exposure to 9,10-PQ with h-PM2.5 and/or h-ASD dose-dependently increased WST-1 reduction at 1 μM compared to the corresponding control while markedly decreasing it at 10 μM. Higher early apoptotic, late apoptotic, or necrotic cell numbers were detected in 9,10-PQ + h-PM2.5 exposure than in 9,10-PQ + h-ASD or 9,10-PQ + h-PM2.5 + h-ASD. Additionally, 1,2-NQ + h-PM2.5 exposure also resulted in an increase in cell death compared to 1,2-NQ + h-ASD and 1,2-NQ + h-PM2.5 + h-ASD. Quinones with or without h-PM2.5, h-ASD, or h-PM2.5 + h-ASD significantly increased ROS production, especially with h-PM2.5. Our findings suggest that quinones, at relatively low concentrations, induce cell death synergistically in the presence of h-PM2.5 rather than h-ASD and h-PM2.5 + h-ASD, partially through the induction of apoptosis with increased ROS generation. MDPI 2023-06-23 /pmc/articles/PMC10341977/ /pubmed/37445720 http://dx.doi.org/10.3390/ijms241310544 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Honda, Akiko
Inoue, Ken-ichiro
Higashihara, Makoto
Ichinose, Takamichi
Ueda, Kayo
Takano, Hirohisa
Differential Pattern of Cell Death and ROS Production in Human Airway Epithelial Cells Exposed to Quinones Combined with Heated-PM2.5 and/or Asian Sand Dust
title Differential Pattern of Cell Death and ROS Production in Human Airway Epithelial Cells Exposed to Quinones Combined with Heated-PM2.5 and/or Asian Sand Dust
title_full Differential Pattern of Cell Death and ROS Production in Human Airway Epithelial Cells Exposed to Quinones Combined with Heated-PM2.5 and/or Asian Sand Dust
title_fullStr Differential Pattern of Cell Death and ROS Production in Human Airway Epithelial Cells Exposed to Quinones Combined with Heated-PM2.5 and/or Asian Sand Dust
title_full_unstemmed Differential Pattern of Cell Death and ROS Production in Human Airway Epithelial Cells Exposed to Quinones Combined with Heated-PM2.5 and/or Asian Sand Dust
title_short Differential Pattern of Cell Death and ROS Production in Human Airway Epithelial Cells Exposed to Quinones Combined with Heated-PM2.5 and/or Asian Sand Dust
title_sort differential pattern of cell death and ros production in human airway epithelial cells exposed to quinones combined with heated-pm2.5 and/or asian sand dust
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10341977/
https://www.ncbi.nlm.nih.gov/pubmed/37445720
http://dx.doi.org/10.3390/ijms241310544
work_keys_str_mv AT hondaakiko differentialpatternofcelldeathandrosproductioninhumanairwayepithelialcellsexposedtoquinonescombinedwithheatedpm25andorasiansanddust
AT inouekenichiro differentialpatternofcelldeathandrosproductioninhumanairwayepithelialcellsexposedtoquinonescombinedwithheatedpm25andorasiansanddust
AT higashiharamakoto differentialpatternofcelldeathandrosproductioninhumanairwayepithelialcellsexposedtoquinonescombinedwithheatedpm25andorasiansanddust
AT ichinosetakamichi differentialpatternofcelldeathandrosproductioninhumanairwayepithelialcellsexposedtoquinonescombinedwithheatedpm25andorasiansanddust
AT uedakayo differentialpatternofcelldeathandrosproductioninhumanairwayepithelialcellsexposedtoquinonescombinedwithheatedpm25andorasiansanddust
AT takanohirohisa differentialpatternofcelldeathandrosproductioninhumanairwayepithelialcellsexposedtoquinonescombinedwithheatedpm25andorasiansanddust