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Examining the Effects of Gestational Physical Activity and Hofbauer Cell Polarization on Angiogenic Factors

While gestational physical activity (PA) has demonstrated health benefits for both birthing parent and fetus, the mechanisms still need to be fully understood. Placental macrophages, or Hofbauer cells (HBCs), comprise a heterogenous population containing inflammatory (CD206-) and anti-inflammatory (...

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Autores principales: Goudreau, Alexandra D., Tanara, Layli, Tzaneva, Velislava, Adamo, Kristi B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10342061/
https://www.ncbi.nlm.nih.gov/pubmed/37444145
http://dx.doi.org/10.3390/ijerph20136298
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author Goudreau, Alexandra D.
Tanara, Layli
Tzaneva, Velislava
Adamo, Kristi B.
author_facet Goudreau, Alexandra D.
Tanara, Layli
Tzaneva, Velislava
Adamo, Kristi B.
author_sort Goudreau, Alexandra D.
collection PubMed
description While gestational physical activity (PA) has demonstrated health benefits for both birthing parent and fetus, the mechanisms still need to be fully understood. Placental macrophages, or Hofbauer cells (HBCs), comprise a heterogenous population containing inflammatory (CD206-) and anti-inflammatory (CD206+) phenotypes. Similar to other tissue-resident macrophages (TRMs), HBCs are potential mediators of angiogenesis due to their secretion of both pro- and anti-angiogenic factors, including FGF2, VEGF, and SPRY2. While PA is associated with an increase in the proportion of VEGF- and FGF2-producing CD206+ macrophages in other tissues, the phenotypes producing FGF2, VEGF, and SPRY2 in the placenta and the associated relationships with gestational PA have not been studied. Using accelerometry, pregnant participants were classified as physically active or inactive in mid- and late-gestation. Term placenta tissue was collected at delivery and used for Western blotting and immunofluorescence to examine the protein expression of FGF2 and SPRY2, and to localize FGF2 in histological samples, respectively. Primary cultures of HBCs were used to examine the phenotypic differences in FGF2, SPRY2, and VEGF production. While no differences in the placental expression of SPRY2, total FGF2, or high-molecular-weight FGF2 were observed based on PA status, active individuals had significantly reduced levels of low-molecular-weight FGF2. Additionally, HBCs of all polarizations produce VEGF, FGF2, and SPRY2, and can form intercellular junctions and multinucleated giant cells. These findings suggest a possible relationship between PA and HBC-driven angiogenesis, providing an avenue for future exploration.
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spelling pubmed-103420612023-07-14 Examining the Effects of Gestational Physical Activity and Hofbauer Cell Polarization on Angiogenic Factors Goudreau, Alexandra D. Tanara, Layli Tzaneva, Velislava Adamo, Kristi B. Int J Environ Res Public Health Article While gestational physical activity (PA) has demonstrated health benefits for both birthing parent and fetus, the mechanisms still need to be fully understood. Placental macrophages, or Hofbauer cells (HBCs), comprise a heterogenous population containing inflammatory (CD206-) and anti-inflammatory (CD206+) phenotypes. Similar to other tissue-resident macrophages (TRMs), HBCs are potential mediators of angiogenesis due to their secretion of both pro- and anti-angiogenic factors, including FGF2, VEGF, and SPRY2. While PA is associated with an increase in the proportion of VEGF- and FGF2-producing CD206+ macrophages in other tissues, the phenotypes producing FGF2, VEGF, and SPRY2 in the placenta and the associated relationships with gestational PA have not been studied. Using accelerometry, pregnant participants were classified as physically active or inactive in mid- and late-gestation. Term placenta tissue was collected at delivery and used for Western blotting and immunofluorescence to examine the protein expression of FGF2 and SPRY2, and to localize FGF2 in histological samples, respectively. Primary cultures of HBCs were used to examine the phenotypic differences in FGF2, SPRY2, and VEGF production. While no differences in the placental expression of SPRY2, total FGF2, or high-molecular-weight FGF2 were observed based on PA status, active individuals had significantly reduced levels of low-molecular-weight FGF2. Additionally, HBCs of all polarizations produce VEGF, FGF2, and SPRY2, and can form intercellular junctions and multinucleated giant cells. These findings suggest a possible relationship between PA and HBC-driven angiogenesis, providing an avenue for future exploration. MDPI 2023-07-04 /pmc/articles/PMC10342061/ /pubmed/37444145 http://dx.doi.org/10.3390/ijerph20136298 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Goudreau, Alexandra D.
Tanara, Layli
Tzaneva, Velislava
Adamo, Kristi B.
Examining the Effects of Gestational Physical Activity and Hofbauer Cell Polarization on Angiogenic Factors
title Examining the Effects of Gestational Physical Activity and Hofbauer Cell Polarization on Angiogenic Factors
title_full Examining the Effects of Gestational Physical Activity and Hofbauer Cell Polarization on Angiogenic Factors
title_fullStr Examining the Effects of Gestational Physical Activity and Hofbauer Cell Polarization on Angiogenic Factors
title_full_unstemmed Examining the Effects of Gestational Physical Activity and Hofbauer Cell Polarization on Angiogenic Factors
title_short Examining the Effects of Gestational Physical Activity and Hofbauer Cell Polarization on Angiogenic Factors
title_sort examining the effects of gestational physical activity and hofbauer cell polarization on angiogenic factors
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10342061/
https://www.ncbi.nlm.nih.gov/pubmed/37444145
http://dx.doi.org/10.3390/ijerph20136298
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