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Dependence of Induced Biological Damage on the Energy Distribution and Intensity of Clinical Intra-Operative Radiotherapy Electron Beams

The survival fraction of epithelial HaCaT cells was analysed to assess the biological damage caused by intraoperative radiotherapy electron beams with varying energy spectra and intensities. These conditions were achieved by irradiating the cells at different depths in water using nominal 6 MeV elec...

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Autores principales: Colmenares, Rafael, Carrión-Marchante, Rebeca, Martín, M. Elena, Salinas Muñoz, Laura, García-Bermejo, María Laura, Oller, Juan C., Muñoz, Antonio, Blanco, Francisco, Rosado, Jaime, Lozano, Ana I., Álvarez, Sofía, García-Vicente, Feliciano, García, Gustavo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10342171/
https://www.ncbi.nlm.nih.gov/pubmed/37445992
http://dx.doi.org/10.3390/ijms241310816
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author Colmenares, Rafael
Carrión-Marchante, Rebeca
Martín, M. Elena
Salinas Muñoz, Laura
García-Bermejo, María Laura
Oller, Juan C.
Muñoz, Antonio
Blanco, Francisco
Rosado, Jaime
Lozano, Ana I.
Álvarez, Sofía
García-Vicente, Feliciano
García, Gustavo
author_facet Colmenares, Rafael
Carrión-Marchante, Rebeca
Martín, M. Elena
Salinas Muñoz, Laura
García-Bermejo, María Laura
Oller, Juan C.
Muñoz, Antonio
Blanco, Francisco
Rosado, Jaime
Lozano, Ana I.
Álvarez, Sofía
García-Vicente, Feliciano
García, Gustavo
author_sort Colmenares, Rafael
collection PubMed
description The survival fraction of epithelial HaCaT cells was analysed to assess the biological damage caused by intraoperative radiotherapy electron beams with varying energy spectra and intensities. These conditions were achieved by irradiating the cells at different depths in water using nominal 6 MeV electron beams while consistently delivering a dose of 5 Gy to the cell layer. Furthermore, a Monte Carlo simulation of the entire irradiation procedure was performed to evaluate the molecular damage in terms of molecular dissociations induced by the radiation. A significant agreement was found between the molecular damage predicted by the simulation and the damage derived from the analysis of the survival fraction. In both cases, a linear relationship was evident, indicating a clear tendency for increased damage as the averaged incident electron energy and intensity decreased for a constant absorbed dose, lowering the dose rate. This trend suggests that the radiation may have a more pronounced impact on surrounding healthy tissues than initially anticipated. However, it is crucial to conduct additional experiments with different target geometries to confirm this tendency and quantify the extent of this effect.
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spelling pubmed-103421712023-07-14 Dependence of Induced Biological Damage on the Energy Distribution and Intensity of Clinical Intra-Operative Radiotherapy Electron Beams Colmenares, Rafael Carrión-Marchante, Rebeca Martín, M. Elena Salinas Muñoz, Laura García-Bermejo, María Laura Oller, Juan C. Muñoz, Antonio Blanco, Francisco Rosado, Jaime Lozano, Ana I. Álvarez, Sofía García-Vicente, Feliciano García, Gustavo Int J Mol Sci Article The survival fraction of epithelial HaCaT cells was analysed to assess the biological damage caused by intraoperative radiotherapy electron beams with varying energy spectra and intensities. These conditions were achieved by irradiating the cells at different depths in water using nominal 6 MeV electron beams while consistently delivering a dose of 5 Gy to the cell layer. Furthermore, a Monte Carlo simulation of the entire irradiation procedure was performed to evaluate the molecular damage in terms of molecular dissociations induced by the radiation. A significant agreement was found between the molecular damage predicted by the simulation and the damage derived from the analysis of the survival fraction. In both cases, a linear relationship was evident, indicating a clear tendency for increased damage as the averaged incident electron energy and intensity decreased for a constant absorbed dose, lowering the dose rate. This trend suggests that the radiation may have a more pronounced impact on surrounding healthy tissues than initially anticipated. However, it is crucial to conduct additional experiments with different target geometries to confirm this tendency and quantify the extent of this effect. MDPI 2023-06-28 /pmc/articles/PMC10342171/ /pubmed/37445992 http://dx.doi.org/10.3390/ijms241310816 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Colmenares, Rafael
Carrión-Marchante, Rebeca
Martín, M. Elena
Salinas Muñoz, Laura
García-Bermejo, María Laura
Oller, Juan C.
Muñoz, Antonio
Blanco, Francisco
Rosado, Jaime
Lozano, Ana I.
Álvarez, Sofía
García-Vicente, Feliciano
García, Gustavo
Dependence of Induced Biological Damage on the Energy Distribution and Intensity of Clinical Intra-Operative Radiotherapy Electron Beams
title Dependence of Induced Biological Damage on the Energy Distribution and Intensity of Clinical Intra-Operative Radiotherapy Electron Beams
title_full Dependence of Induced Biological Damage on the Energy Distribution and Intensity of Clinical Intra-Operative Radiotherapy Electron Beams
title_fullStr Dependence of Induced Biological Damage on the Energy Distribution and Intensity of Clinical Intra-Operative Radiotherapy Electron Beams
title_full_unstemmed Dependence of Induced Biological Damage on the Energy Distribution and Intensity of Clinical Intra-Operative Radiotherapy Electron Beams
title_short Dependence of Induced Biological Damage on the Energy Distribution and Intensity of Clinical Intra-Operative Radiotherapy Electron Beams
title_sort dependence of induced biological damage on the energy distribution and intensity of clinical intra-operative radiotherapy electron beams
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10342171/
https://www.ncbi.nlm.nih.gov/pubmed/37445992
http://dx.doi.org/10.3390/ijms241310816
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