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Transcriptome Landscape Analyses of the Regulatory Network for Zygotic Embryo Development in Paeonia ostii
Paeonia ostii is a worldwide ornamental flower and an emerging oil crop. Zyotic embryogenesis is a critical process during seed development, and it can provide a basis for improving the efficiency of somatic embryogenesis (SE). In this study, transcriptome sequencing of embryo development was perfor...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10342179/ https://www.ncbi.nlm.nih.gov/pubmed/37445891 http://dx.doi.org/10.3390/ijms241310715 |
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author | Xu, Yufeng Shang, Wenqian Li, Linda Song, Yinglong Wang, Guiqing Shi, Liyun Shen, Yuxiao Sun, Yuke He, Songlin Wang, Zheng |
author_facet | Xu, Yufeng Shang, Wenqian Li, Linda Song, Yinglong Wang, Guiqing Shi, Liyun Shen, Yuxiao Sun, Yuke He, Songlin Wang, Zheng |
author_sort | Xu, Yufeng |
collection | PubMed |
description | Paeonia ostii is a worldwide ornamental flower and an emerging oil crop. Zyotic embryogenesis is a critical process during seed development, and it can provide a basis for improving the efficiency of somatic embryogenesis (SE). In this study, transcriptome sequencing of embryo development was performed to investigate gene expression profiling in P. ostii and identified Differentially expressed genes (DEGs) related to transcription factors, plant hormones, and antioxidant enzymes. The results indicated that IAA (Indole-3-acetic acid), GA (Gibberellin), BR (Brassinosteroid) and ETH (Ethylene) were beneficial to early embryonic morphogenesis, while CTK (Cytokinin) and ABA (Abscisic Acid) promoted embryo morphogenesis and maturation. The antioxidant enzymes’ activity was the highest in early embryos and an important participant in embryo formation. The high expression of the genes encoding fatty acid desaturase was beneficial to fast oil accumulation. Representative DEGs were selected and validated using qRT-PCR. Protein-protein interaction network (PPI) was predicted, and six central node proteins, including AUX1, PIN1, ARF6, LAX3, ABCB19, PIF3, and PIF4, were screened. Our results provided new insights into the formation of embryo development and even somatic embryo development in tree peonies. |
format | Online Article Text |
id | pubmed-10342179 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-103421792023-07-14 Transcriptome Landscape Analyses of the Regulatory Network for Zygotic Embryo Development in Paeonia ostii Xu, Yufeng Shang, Wenqian Li, Linda Song, Yinglong Wang, Guiqing Shi, Liyun Shen, Yuxiao Sun, Yuke He, Songlin Wang, Zheng Int J Mol Sci Article Paeonia ostii is a worldwide ornamental flower and an emerging oil crop. Zyotic embryogenesis is a critical process during seed development, and it can provide a basis for improving the efficiency of somatic embryogenesis (SE). In this study, transcriptome sequencing of embryo development was performed to investigate gene expression profiling in P. ostii and identified Differentially expressed genes (DEGs) related to transcription factors, plant hormones, and antioxidant enzymes. The results indicated that IAA (Indole-3-acetic acid), GA (Gibberellin), BR (Brassinosteroid) and ETH (Ethylene) were beneficial to early embryonic morphogenesis, while CTK (Cytokinin) and ABA (Abscisic Acid) promoted embryo morphogenesis and maturation. The antioxidant enzymes’ activity was the highest in early embryos and an important participant in embryo formation. The high expression of the genes encoding fatty acid desaturase was beneficial to fast oil accumulation. Representative DEGs were selected and validated using qRT-PCR. Protein-protein interaction network (PPI) was predicted, and six central node proteins, including AUX1, PIN1, ARF6, LAX3, ABCB19, PIF3, and PIF4, were screened. Our results provided new insights into the formation of embryo development and even somatic embryo development in tree peonies. MDPI 2023-06-27 /pmc/articles/PMC10342179/ /pubmed/37445891 http://dx.doi.org/10.3390/ijms241310715 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Xu, Yufeng Shang, Wenqian Li, Linda Song, Yinglong Wang, Guiqing Shi, Liyun Shen, Yuxiao Sun, Yuke He, Songlin Wang, Zheng Transcriptome Landscape Analyses of the Regulatory Network for Zygotic Embryo Development in Paeonia ostii |
title | Transcriptome Landscape Analyses of the Regulatory Network for Zygotic Embryo Development in Paeonia ostii |
title_full | Transcriptome Landscape Analyses of the Regulatory Network for Zygotic Embryo Development in Paeonia ostii |
title_fullStr | Transcriptome Landscape Analyses of the Regulatory Network for Zygotic Embryo Development in Paeonia ostii |
title_full_unstemmed | Transcriptome Landscape Analyses of the Regulatory Network for Zygotic Embryo Development in Paeonia ostii |
title_short | Transcriptome Landscape Analyses of the Regulatory Network for Zygotic Embryo Development in Paeonia ostii |
title_sort | transcriptome landscape analyses of the regulatory network for zygotic embryo development in paeonia ostii |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10342179/ https://www.ncbi.nlm.nih.gov/pubmed/37445891 http://dx.doi.org/10.3390/ijms241310715 |
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