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Interplay between the RNA Chaperone Hfq, Small RNAs and Transcriptional Regulator OmpR Modulates Iron Homeostasis in the Enteropathogen Yersinia enterocolitica

Iron is both essential for and potentially toxic to bacteria, so the precise maintenance of iron homeostasis is necessary for their survival. Our previous study indicated that in the human enteropathogen Yersinia enterocolitica, the regulator OmpR directly controls the transcription of the fur, fecA...

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Autores principales: Jaworska, Karolina, Konarska, Julia, Gomza, Patrycja, Rożen, Paula, Nieckarz, Marta, Krawczyk-Balska, Agata, Brzostek, Katarzyna, Raczkowska, Adrianna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10342277/
https://www.ncbi.nlm.nih.gov/pubmed/37446335
http://dx.doi.org/10.3390/ijms241311157
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author Jaworska, Karolina
Konarska, Julia
Gomza, Patrycja
Rożen, Paula
Nieckarz, Marta
Krawczyk-Balska, Agata
Brzostek, Katarzyna
Raczkowska, Adrianna
author_facet Jaworska, Karolina
Konarska, Julia
Gomza, Patrycja
Rożen, Paula
Nieckarz, Marta
Krawczyk-Balska, Agata
Brzostek, Katarzyna
Raczkowska, Adrianna
author_sort Jaworska, Karolina
collection PubMed
description Iron is both essential for and potentially toxic to bacteria, so the precise maintenance of iron homeostasis is necessary for their survival. Our previous study indicated that in the human enteropathogen Yersinia enterocolitica, the regulator OmpR directly controls the transcription of the fur, fecA and fepA genes, encoding the ferric uptake repressor and two transporters of ferric siderophores, respectively. This study was undertaken to determine the significance of the RNA chaperone Hfq and the small RNAs OmrA and RyhB1 in the post-transcriptional control of the expression of these OmpR targets. We show that Hfq silences fur, fecA and fepA expression post-transcriptionally and negatively affects the production of FLAG-tagged Fur, FecA and FepA proteins. In addition, we found that the fur gene is under the negative control of the sRNA RyhB1, while fecA and fepA are negatively regulated by the sRNA OmrA. Finally, our data revealed that the role of OmrA results from a complex interplay of transcriptional and post-transcriptional effects in the feedback circuit between the regulator OmpR and the sRNA OmrA. Thus, the expression of fur, fecA and fepA is subject to complex transcriptional and post-transcriptional regulation in order to maintain iron homeostasis in Y. enterocolitica.
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spelling pubmed-103422772023-07-14 Interplay between the RNA Chaperone Hfq, Small RNAs and Transcriptional Regulator OmpR Modulates Iron Homeostasis in the Enteropathogen Yersinia enterocolitica Jaworska, Karolina Konarska, Julia Gomza, Patrycja Rożen, Paula Nieckarz, Marta Krawczyk-Balska, Agata Brzostek, Katarzyna Raczkowska, Adrianna Int J Mol Sci Article Iron is both essential for and potentially toxic to bacteria, so the precise maintenance of iron homeostasis is necessary for their survival. Our previous study indicated that in the human enteropathogen Yersinia enterocolitica, the regulator OmpR directly controls the transcription of the fur, fecA and fepA genes, encoding the ferric uptake repressor and two transporters of ferric siderophores, respectively. This study was undertaken to determine the significance of the RNA chaperone Hfq and the small RNAs OmrA and RyhB1 in the post-transcriptional control of the expression of these OmpR targets. We show that Hfq silences fur, fecA and fepA expression post-transcriptionally and negatively affects the production of FLAG-tagged Fur, FecA and FepA proteins. In addition, we found that the fur gene is under the negative control of the sRNA RyhB1, while fecA and fepA are negatively regulated by the sRNA OmrA. Finally, our data revealed that the role of OmrA results from a complex interplay of transcriptional and post-transcriptional effects in the feedback circuit between the regulator OmpR and the sRNA OmrA. Thus, the expression of fur, fecA and fepA is subject to complex transcriptional and post-transcriptional regulation in order to maintain iron homeostasis in Y. enterocolitica. MDPI 2023-07-06 /pmc/articles/PMC10342277/ /pubmed/37446335 http://dx.doi.org/10.3390/ijms241311157 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jaworska, Karolina
Konarska, Julia
Gomza, Patrycja
Rożen, Paula
Nieckarz, Marta
Krawczyk-Balska, Agata
Brzostek, Katarzyna
Raczkowska, Adrianna
Interplay between the RNA Chaperone Hfq, Small RNAs and Transcriptional Regulator OmpR Modulates Iron Homeostasis in the Enteropathogen Yersinia enterocolitica
title Interplay between the RNA Chaperone Hfq, Small RNAs and Transcriptional Regulator OmpR Modulates Iron Homeostasis in the Enteropathogen Yersinia enterocolitica
title_full Interplay between the RNA Chaperone Hfq, Small RNAs and Transcriptional Regulator OmpR Modulates Iron Homeostasis in the Enteropathogen Yersinia enterocolitica
title_fullStr Interplay between the RNA Chaperone Hfq, Small RNAs and Transcriptional Regulator OmpR Modulates Iron Homeostasis in the Enteropathogen Yersinia enterocolitica
title_full_unstemmed Interplay between the RNA Chaperone Hfq, Small RNAs and Transcriptional Regulator OmpR Modulates Iron Homeostasis in the Enteropathogen Yersinia enterocolitica
title_short Interplay between the RNA Chaperone Hfq, Small RNAs and Transcriptional Regulator OmpR Modulates Iron Homeostasis in the Enteropathogen Yersinia enterocolitica
title_sort interplay between the rna chaperone hfq, small rnas and transcriptional regulator ompr modulates iron homeostasis in the enteropathogen yersinia enterocolitica
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10342277/
https://www.ncbi.nlm.nih.gov/pubmed/37446335
http://dx.doi.org/10.3390/ijms241311157
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