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Prokineticin 2 and Cytokine Content in the Synovial Fluid of Knee Osteoarthritis and Traumatic Meniscal Tear Patients: Preliminary Results

Knee osteoarthritis (OA) is a chronic degenerative inflammatory-based condition caused by a cascade of different intra-articular molecules including several cytokines. Among the cytokines, prokineticins (PKs) have recently been identified as important mediators of inflammation and pain. This observa...

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Autores principales: Turati, Marco, Franchi, Silvia, Crippa, Marco, Rizzi, Laura, Rigamonti, Luca, Sacerdote, Paola, Gatti, Simone Daniel, Piatti, Massimiliano, Galimberti, Giulia, Munegato, Daniele, Amodeo, Giada, Omeljaniuk, Robert J., Zatti, Giovanni, Torsello, Antonio, Bigoni, Marco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10342294/
https://www.ncbi.nlm.nih.gov/pubmed/37445367
http://dx.doi.org/10.3390/jcm12134330
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author Turati, Marco
Franchi, Silvia
Crippa, Marco
Rizzi, Laura
Rigamonti, Luca
Sacerdote, Paola
Gatti, Simone Daniel
Piatti, Massimiliano
Galimberti, Giulia
Munegato, Daniele
Amodeo, Giada
Omeljaniuk, Robert J.
Zatti, Giovanni
Torsello, Antonio
Bigoni, Marco
author_facet Turati, Marco
Franchi, Silvia
Crippa, Marco
Rizzi, Laura
Rigamonti, Luca
Sacerdote, Paola
Gatti, Simone Daniel
Piatti, Massimiliano
Galimberti, Giulia
Munegato, Daniele
Amodeo, Giada
Omeljaniuk, Robert J.
Zatti, Giovanni
Torsello, Antonio
Bigoni, Marco
author_sort Turati, Marco
collection PubMed
description Knee osteoarthritis (OA) is a chronic degenerative inflammatory-based condition caused by a cascade of different intra-articular molecules including several cytokines. Among the cytokines, prokineticins (PKs) have recently been identified as important mediators of inflammation and pain. This observational study examined the potential involvement of PK2 in degenerative or traumatic knee disease. Fifteen patients presenting knee osteoarthritis (OA group) and 15 patients presenting a traumatic meniscal tear (TM group) were studied. Synovial fluid samples from affected knees were assessed for PK2, IL-10, and TNF-α using the ELISA method. At a long-term follow-up (minimum 5 years, mean = 6.1 years), patients in the TM group underwent clinical re-evaluation with PROMs (Tegner Activity Scale, IKDC, Lysholm, SKV); in addition, X-ray visualization was used to assess the presence of secondary OA. PK2 was detected in synovial fluids of both TM and OA patients and the levels were comparable between the two groups, while IL-10 levels were significantly greater in the OA group than those in TM patients. PK2 levels correlated with those of IL-10. PK2 levels were greater in blood effusions compared to clear samples, did not differ significantly between sexes, nor were they related to differences in weight, height, or injury (meniscal laterality, time since dosing). No correlation was found between PROMs and radiological classifications in patients in the TM group at final follow-up. These data are the first observations of PK2 in synovial fluid following traumatic meniscus injury. These findings suggest possible further prognostic indices and therapeutic targets to limit the development of secondary OA.
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spelling pubmed-103422942023-07-14 Prokineticin 2 and Cytokine Content in the Synovial Fluid of Knee Osteoarthritis and Traumatic Meniscal Tear Patients: Preliminary Results Turati, Marco Franchi, Silvia Crippa, Marco Rizzi, Laura Rigamonti, Luca Sacerdote, Paola Gatti, Simone Daniel Piatti, Massimiliano Galimberti, Giulia Munegato, Daniele Amodeo, Giada Omeljaniuk, Robert J. Zatti, Giovanni Torsello, Antonio Bigoni, Marco J Clin Med Article Knee osteoarthritis (OA) is a chronic degenerative inflammatory-based condition caused by a cascade of different intra-articular molecules including several cytokines. Among the cytokines, prokineticins (PKs) have recently been identified as important mediators of inflammation and pain. This observational study examined the potential involvement of PK2 in degenerative or traumatic knee disease. Fifteen patients presenting knee osteoarthritis (OA group) and 15 patients presenting a traumatic meniscal tear (TM group) were studied. Synovial fluid samples from affected knees were assessed for PK2, IL-10, and TNF-α using the ELISA method. At a long-term follow-up (minimum 5 years, mean = 6.1 years), patients in the TM group underwent clinical re-evaluation with PROMs (Tegner Activity Scale, IKDC, Lysholm, SKV); in addition, X-ray visualization was used to assess the presence of secondary OA. PK2 was detected in synovial fluids of both TM and OA patients and the levels were comparable between the two groups, while IL-10 levels were significantly greater in the OA group than those in TM patients. PK2 levels correlated with those of IL-10. PK2 levels were greater in blood effusions compared to clear samples, did not differ significantly between sexes, nor were they related to differences in weight, height, or injury (meniscal laterality, time since dosing). No correlation was found between PROMs and radiological classifications in patients in the TM group at final follow-up. These data are the first observations of PK2 in synovial fluid following traumatic meniscus injury. These findings suggest possible further prognostic indices and therapeutic targets to limit the development of secondary OA. MDPI 2023-06-28 /pmc/articles/PMC10342294/ /pubmed/37445367 http://dx.doi.org/10.3390/jcm12134330 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Turati, Marco
Franchi, Silvia
Crippa, Marco
Rizzi, Laura
Rigamonti, Luca
Sacerdote, Paola
Gatti, Simone Daniel
Piatti, Massimiliano
Galimberti, Giulia
Munegato, Daniele
Amodeo, Giada
Omeljaniuk, Robert J.
Zatti, Giovanni
Torsello, Antonio
Bigoni, Marco
Prokineticin 2 and Cytokine Content in the Synovial Fluid of Knee Osteoarthritis and Traumatic Meniscal Tear Patients: Preliminary Results
title Prokineticin 2 and Cytokine Content in the Synovial Fluid of Knee Osteoarthritis and Traumatic Meniscal Tear Patients: Preliminary Results
title_full Prokineticin 2 and Cytokine Content in the Synovial Fluid of Knee Osteoarthritis and Traumatic Meniscal Tear Patients: Preliminary Results
title_fullStr Prokineticin 2 and Cytokine Content in the Synovial Fluid of Knee Osteoarthritis and Traumatic Meniscal Tear Patients: Preliminary Results
title_full_unstemmed Prokineticin 2 and Cytokine Content in the Synovial Fluid of Knee Osteoarthritis and Traumatic Meniscal Tear Patients: Preliminary Results
title_short Prokineticin 2 and Cytokine Content in the Synovial Fluid of Knee Osteoarthritis and Traumatic Meniscal Tear Patients: Preliminary Results
title_sort prokineticin 2 and cytokine content in the synovial fluid of knee osteoarthritis and traumatic meniscal tear patients: preliminary results
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10342294/
https://www.ncbi.nlm.nih.gov/pubmed/37445367
http://dx.doi.org/10.3390/jcm12134330
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