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Developmental Neurotoxicity of Trichlorfon in Zebrafish Larvae

Trichlorfon is an organophosphorus pesticide widely used in aquaculture and has potential neurotoxicity, but the underlying mechanism remains unclear. In the present study, zebrafish embryos were exposed to trichlorfon at concentrations (0, 0.1, 2 and 5 mg/L) used in aquaculture from 2 to 144 h post...

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Autores principales: Shi, Qipeng, Yang, Huaran, Chen, Yangli, Zheng, Na, Li, Xiaoyu, Wang, Xianfeng, Ding, Weikai, Zhang, Bangjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10342510/
https://www.ncbi.nlm.nih.gov/pubmed/37446277
http://dx.doi.org/10.3390/ijms241311099
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author Shi, Qipeng
Yang, Huaran
Chen, Yangli
Zheng, Na
Li, Xiaoyu
Wang, Xianfeng
Ding, Weikai
Zhang, Bangjun
author_facet Shi, Qipeng
Yang, Huaran
Chen, Yangli
Zheng, Na
Li, Xiaoyu
Wang, Xianfeng
Ding, Weikai
Zhang, Bangjun
author_sort Shi, Qipeng
collection PubMed
description Trichlorfon is an organophosphorus pesticide widely used in aquaculture and has potential neurotoxicity, but the underlying mechanism remains unclear. In the present study, zebrafish embryos were exposed to trichlorfon at concentrations (0, 0.1, 2 and 5 mg/L) used in aquaculture from 2 to 144 h post fertilization. Trichlorfon exposure reduced the survival rate, hatching rate, heartbeat and body length and increased the malformation rate of zebrafish larvae. The locomotor activity of larvae was significantly reduced. The results of molecular docking revealed that trichlorfon could bind to acetylcholinesterase (AChE). Furthermore, trichlorfon significantly inhibited AChE activity, accompanied by decreased acetylcholine, dopamine and serotonin content in larvae. The transcription patterns of genes related to acetylcholine (e.g., ache, chrna7, chata, hact and vacht), dopamine (e.g., drd4a and drd4b) and serotonin systems (e.g., tph1, tph2, tphr, serta, sertb, htrlaa and htrlab) were consistent with the changes in acetylcholine, dopamine, serotonin content and AChE activity. The genes related to the central nervous system (CNS) (e.g., a1-tubulin, mbp, syn2a, shha and gap-43) were downregulated. Our results indicate that the developmental neurotoxicity of trichlorfon might be attributed to disorders of cholinergic, dopaminergic and serotonergic signaling and the development of the CNS.
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spelling pubmed-103425102023-07-14 Developmental Neurotoxicity of Trichlorfon in Zebrafish Larvae Shi, Qipeng Yang, Huaran Chen, Yangli Zheng, Na Li, Xiaoyu Wang, Xianfeng Ding, Weikai Zhang, Bangjun Int J Mol Sci Article Trichlorfon is an organophosphorus pesticide widely used in aquaculture and has potential neurotoxicity, but the underlying mechanism remains unclear. In the present study, zebrafish embryos were exposed to trichlorfon at concentrations (0, 0.1, 2 and 5 mg/L) used in aquaculture from 2 to 144 h post fertilization. Trichlorfon exposure reduced the survival rate, hatching rate, heartbeat and body length and increased the malformation rate of zebrafish larvae. The locomotor activity of larvae was significantly reduced. The results of molecular docking revealed that trichlorfon could bind to acetylcholinesterase (AChE). Furthermore, trichlorfon significantly inhibited AChE activity, accompanied by decreased acetylcholine, dopamine and serotonin content in larvae. The transcription patterns of genes related to acetylcholine (e.g., ache, chrna7, chata, hact and vacht), dopamine (e.g., drd4a and drd4b) and serotonin systems (e.g., tph1, tph2, tphr, serta, sertb, htrlaa and htrlab) were consistent with the changes in acetylcholine, dopamine, serotonin content and AChE activity. The genes related to the central nervous system (CNS) (e.g., a1-tubulin, mbp, syn2a, shha and gap-43) were downregulated. Our results indicate that the developmental neurotoxicity of trichlorfon might be attributed to disorders of cholinergic, dopaminergic and serotonergic signaling and the development of the CNS. MDPI 2023-07-04 /pmc/articles/PMC10342510/ /pubmed/37446277 http://dx.doi.org/10.3390/ijms241311099 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shi, Qipeng
Yang, Huaran
Chen, Yangli
Zheng, Na
Li, Xiaoyu
Wang, Xianfeng
Ding, Weikai
Zhang, Bangjun
Developmental Neurotoxicity of Trichlorfon in Zebrafish Larvae
title Developmental Neurotoxicity of Trichlorfon in Zebrafish Larvae
title_full Developmental Neurotoxicity of Trichlorfon in Zebrafish Larvae
title_fullStr Developmental Neurotoxicity of Trichlorfon in Zebrafish Larvae
title_full_unstemmed Developmental Neurotoxicity of Trichlorfon in Zebrafish Larvae
title_short Developmental Neurotoxicity of Trichlorfon in Zebrafish Larvae
title_sort developmental neurotoxicity of trichlorfon in zebrafish larvae
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10342510/
https://www.ncbi.nlm.nih.gov/pubmed/37446277
http://dx.doi.org/10.3390/ijms241311099
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