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Opposite Auxin Dynamics Determine the Gametophytic and Embryogenic Fates of the Microspore

The microspore can follow two different developmental pathways. In vivo microspores follow the gametophytic program to produce pollen grains. In vitro, isolated microspores can be reprogrammed by stress treatments and follow the embryogenic program, producing doubled-haploid embryos. In the present...

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Autores principales: Pérez-Pérez, Yolanda, Solís, María Teresa, Albacete, Alfonso, Testillano, Pilar S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10342522/
https://www.ncbi.nlm.nih.gov/pubmed/37446349
http://dx.doi.org/10.3390/ijms241311177
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author Pérez-Pérez, Yolanda
Solís, María Teresa
Albacete, Alfonso
Testillano, Pilar S.
author_facet Pérez-Pérez, Yolanda
Solís, María Teresa
Albacete, Alfonso
Testillano, Pilar S.
author_sort Pérez-Pérez, Yolanda
collection PubMed
description The microspore can follow two different developmental pathways. In vivo microspores follow the gametophytic program to produce pollen grains. In vitro, isolated microspores can be reprogrammed by stress treatments and follow the embryogenic program, producing doubled-haploid embryos. In the present study, we analyzed the dynamics and role of endogenous auxin in microspore development during these two different scenarios, in Brassica napus. We analyzed auxin concentration, cellular accumulation, the expression of the TAA1 auxin biosynthesis gene, and the PIN1-like efflux carrier gene, as well as the effects of inhibiting auxin biosynthesis by kynurenine on microspore embryogenesis. During the gametophytic pathway, auxin levels and TAA1 and PIN1-like expression were high at early stages, in tetrads and tapetum, while they progressively decreased during gametogenesis in both pollen and tapetum cells. In contrast, in microspore embryogenesis, TAA1 and PIN1-like genes were upregulated, and auxin concentration increased from the first embryogenic divisions. Kynurenine treatment decreased both embryogenesis induction and embryo production, indicating that auxin biosynthesis is required for microspore embryogenesis initiation and progression. The findings indicate that auxin exhibits two opposite profiles during these two microspore developmental pathways, which determine the different cell fates of the microspore.
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spelling pubmed-103425222023-07-14 Opposite Auxin Dynamics Determine the Gametophytic and Embryogenic Fates of the Microspore Pérez-Pérez, Yolanda Solís, María Teresa Albacete, Alfonso Testillano, Pilar S. Int J Mol Sci Article The microspore can follow two different developmental pathways. In vivo microspores follow the gametophytic program to produce pollen grains. In vitro, isolated microspores can be reprogrammed by stress treatments and follow the embryogenic program, producing doubled-haploid embryos. In the present study, we analyzed the dynamics and role of endogenous auxin in microspore development during these two different scenarios, in Brassica napus. We analyzed auxin concentration, cellular accumulation, the expression of the TAA1 auxin biosynthesis gene, and the PIN1-like efflux carrier gene, as well as the effects of inhibiting auxin biosynthesis by kynurenine on microspore embryogenesis. During the gametophytic pathway, auxin levels and TAA1 and PIN1-like expression were high at early stages, in tetrads and tapetum, while they progressively decreased during gametogenesis in both pollen and tapetum cells. In contrast, in microspore embryogenesis, TAA1 and PIN1-like genes were upregulated, and auxin concentration increased from the first embryogenic divisions. Kynurenine treatment decreased both embryogenesis induction and embryo production, indicating that auxin biosynthesis is required for microspore embryogenesis initiation and progression. The findings indicate that auxin exhibits two opposite profiles during these two microspore developmental pathways, which determine the different cell fates of the microspore. MDPI 2023-07-06 /pmc/articles/PMC10342522/ /pubmed/37446349 http://dx.doi.org/10.3390/ijms241311177 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pérez-Pérez, Yolanda
Solís, María Teresa
Albacete, Alfonso
Testillano, Pilar S.
Opposite Auxin Dynamics Determine the Gametophytic and Embryogenic Fates of the Microspore
title Opposite Auxin Dynamics Determine the Gametophytic and Embryogenic Fates of the Microspore
title_full Opposite Auxin Dynamics Determine the Gametophytic and Embryogenic Fates of the Microspore
title_fullStr Opposite Auxin Dynamics Determine the Gametophytic and Embryogenic Fates of the Microspore
title_full_unstemmed Opposite Auxin Dynamics Determine the Gametophytic and Embryogenic Fates of the Microspore
title_short Opposite Auxin Dynamics Determine the Gametophytic and Embryogenic Fates of the Microspore
title_sort opposite auxin dynamics determine the gametophytic and embryogenic fates of the microspore
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10342522/
https://www.ncbi.nlm.nih.gov/pubmed/37446349
http://dx.doi.org/10.3390/ijms241311177
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