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Study on Extraction, Physicochemical Properties, and Bacterio-Static Activity of Polysaccharides from Phellinus linteus

We optimized an ultrasound-assisted extraction process of Phellinus linteus mycelium polysaccharides (PLPs) and studied their monosaccharide composition and bacteriostatic properties. Based on a single-factor experiment, a three-factor, three-level Box–Behnken design was used to optimize the ultraso...

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Autores principales: Gao, Nengbin, Zhang, Weijia, Hu, Dianjie, Lin, Guo, Wang, Jingxuan, Xue, Feng, Wang, Qian, Zhao, Hongfei, Dou, Xin, Zhang, Lihong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10343582/
https://www.ncbi.nlm.nih.gov/pubmed/37446762
http://dx.doi.org/10.3390/molecules28135102
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author Gao, Nengbin
Zhang, Weijia
Hu, Dianjie
Lin, Guo
Wang, Jingxuan
Xue, Feng
Wang, Qian
Zhao, Hongfei
Dou, Xin
Zhang, Lihong
author_facet Gao, Nengbin
Zhang, Weijia
Hu, Dianjie
Lin, Guo
Wang, Jingxuan
Xue, Feng
Wang, Qian
Zhao, Hongfei
Dou, Xin
Zhang, Lihong
author_sort Gao, Nengbin
collection PubMed
description We optimized an ultrasound-assisted extraction process of Phellinus linteus mycelium polysaccharides (PLPs) and studied their monosaccharide composition and bacteriostatic properties. Based on a single-factor experiment, a three-factor, three-level Box–Behnken design was used to optimize the ultrasound-assisted extraction process of PLP, using the yield of PLP as the index. The chemical composition and monosaccharide composition of PLP were determined by chemical analysis and HPLC analysis, respectively. Microscopic morphological analysis of the surface of PLP was performed via swept-surface electron microscopy. The bacteriostatic properties of PLP were determined using the spectrophotometric turbidimetric method. The results showed that the best extraction process of PLP with ultrasonic assistance achieved a result of 1:42 g/mL. In this method, the ultrasonic temperature was 60 °C, ultrasonic extraction was performed for 20 min, and the yield of PLP was 12.98%. The monosaccharide composition of PLP mainly contains glucose (Glc), mannose (Man), galactose (Gal), and glucuronic acid (GlcA). The intracellular polysaccharide of Phellinus igniarius Mycelia (PIP) is an irregular spherical accumulation, the surface is rough and not smooth, and the extracellular polysaccharide (PEP) is a crumbly accumulation. PIP has a stronger inhibitory ability for S. aureus and E. coli and a slightly weaker inhibitory effect for B. subtilis; the inhibitory effect of PEP on S. aureus, E. coli, and B. subtilis is slightly inferior to that of PIP.
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spelling pubmed-103435822023-07-14 Study on Extraction, Physicochemical Properties, and Bacterio-Static Activity of Polysaccharides from Phellinus linteus Gao, Nengbin Zhang, Weijia Hu, Dianjie Lin, Guo Wang, Jingxuan Xue, Feng Wang, Qian Zhao, Hongfei Dou, Xin Zhang, Lihong Molecules Article We optimized an ultrasound-assisted extraction process of Phellinus linteus mycelium polysaccharides (PLPs) and studied their monosaccharide composition and bacteriostatic properties. Based on a single-factor experiment, a three-factor, three-level Box–Behnken design was used to optimize the ultrasound-assisted extraction process of PLP, using the yield of PLP as the index. The chemical composition and monosaccharide composition of PLP were determined by chemical analysis and HPLC analysis, respectively. Microscopic morphological analysis of the surface of PLP was performed via swept-surface electron microscopy. The bacteriostatic properties of PLP were determined using the spectrophotometric turbidimetric method. The results showed that the best extraction process of PLP with ultrasonic assistance achieved a result of 1:42 g/mL. In this method, the ultrasonic temperature was 60 °C, ultrasonic extraction was performed for 20 min, and the yield of PLP was 12.98%. The monosaccharide composition of PLP mainly contains glucose (Glc), mannose (Man), galactose (Gal), and glucuronic acid (GlcA). The intracellular polysaccharide of Phellinus igniarius Mycelia (PIP) is an irregular spherical accumulation, the surface is rough and not smooth, and the extracellular polysaccharide (PEP) is a crumbly accumulation. PIP has a stronger inhibitory ability for S. aureus and E. coli and a slightly weaker inhibitory effect for B. subtilis; the inhibitory effect of PEP on S. aureus, E. coli, and B. subtilis is slightly inferior to that of PIP. MDPI 2023-06-29 /pmc/articles/PMC10343582/ /pubmed/37446762 http://dx.doi.org/10.3390/molecules28135102 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gao, Nengbin
Zhang, Weijia
Hu, Dianjie
Lin, Guo
Wang, Jingxuan
Xue, Feng
Wang, Qian
Zhao, Hongfei
Dou, Xin
Zhang, Lihong
Study on Extraction, Physicochemical Properties, and Bacterio-Static Activity of Polysaccharides from Phellinus linteus
title Study on Extraction, Physicochemical Properties, and Bacterio-Static Activity of Polysaccharides from Phellinus linteus
title_full Study on Extraction, Physicochemical Properties, and Bacterio-Static Activity of Polysaccharides from Phellinus linteus
title_fullStr Study on Extraction, Physicochemical Properties, and Bacterio-Static Activity of Polysaccharides from Phellinus linteus
title_full_unstemmed Study on Extraction, Physicochemical Properties, and Bacterio-Static Activity of Polysaccharides from Phellinus linteus
title_short Study on Extraction, Physicochemical Properties, and Bacterio-Static Activity of Polysaccharides from Phellinus linteus
title_sort study on extraction, physicochemical properties, and bacterio-static activity of polysaccharides from phellinus linteus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10343582/
https://www.ncbi.nlm.nih.gov/pubmed/37446762
http://dx.doi.org/10.3390/molecules28135102
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