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Datura stramonium Flowers as a Potential Natural Resource of Bioactive Molecules: Identification of Anti-Inflammatory Agents and Molecular Docking Analysis
The present study investigated the antioxidant, antibacterial, antiviral and anti-inflammatory activities of different aerial parts (flowers, leaves and seeds) of Datura stramonium. The plant material was extracted with 80% methanol for about 24 h. The sensitivity to microorganisms analysis was perf...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10343631/ https://www.ncbi.nlm.nih.gov/pubmed/37446858 http://dx.doi.org/10.3390/molecules28135195 |
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author | Damergi, Bilel Essid, Rym Fares, Nadia Khadraoui, Nadine Ageitos, Lucía Ben Alaya, Ameni Gharbi, Dorra Abid, Islem Rashed Alothman, Monerah Limam, Ferid Rodríguez, Jaime Jiménez, Carlos Tabbene, Olfa |
author_facet | Damergi, Bilel Essid, Rym Fares, Nadia Khadraoui, Nadine Ageitos, Lucía Ben Alaya, Ameni Gharbi, Dorra Abid, Islem Rashed Alothman, Monerah Limam, Ferid Rodríguez, Jaime Jiménez, Carlos Tabbene, Olfa |
author_sort | Damergi, Bilel |
collection | PubMed |
description | The present study investigated the antioxidant, antibacterial, antiviral and anti-inflammatory activities of different aerial parts (flowers, leaves and seeds) of Datura stramonium. The plant material was extracted with 80% methanol for about 24 h. The sensitivity to microorganisms analysis was performed by the microdilution technique. Antioxidant tests were performed by scavenging the DPPH and ABTS radicals, and by FRAP assay. Anti-inflammatory activity was evaluated through the inhibition of nitric oxide production in activated macrophage RAW 264.7 cells. Cell viability was assessed with an MTT assay. Results show that the flower extract revealed a powerful antimicrobial capacity against Gram-positive bacteria and strong antioxidant and anti-inflammatory activities. No significant cytotoxicity to activated macrophages was recorded. High resolution electrospray ionization mass spectrometry and nuclear magnetic resonance analysis identified two molecules with important anti-inflammatory effects: 12α-hydroxydaturametelin B and daturametelin B. Molecular docking analysis with both pro-inflammatory agents tumor necrosis factor alpha and interleukin-6 revealed that both compounds showed good binding features with the selected target proteins. Our results suggest that D. stramonium flower is a promising source of compounds with potential antioxidant, antibacterial, and anti-inflammatory activities. Isolated withanolide steroidal lactones from D. stramonium flower extract with promising anti-inflammatory activity have therapeutic potential against inflammatory disorders. |
format | Online Article Text |
id | pubmed-10343631 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-103436312023-07-14 Datura stramonium Flowers as a Potential Natural Resource of Bioactive Molecules: Identification of Anti-Inflammatory Agents and Molecular Docking Analysis Damergi, Bilel Essid, Rym Fares, Nadia Khadraoui, Nadine Ageitos, Lucía Ben Alaya, Ameni Gharbi, Dorra Abid, Islem Rashed Alothman, Monerah Limam, Ferid Rodríguez, Jaime Jiménez, Carlos Tabbene, Olfa Molecules Article The present study investigated the antioxidant, antibacterial, antiviral and anti-inflammatory activities of different aerial parts (flowers, leaves and seeds) of Datura stramonium. The plant material was extracted with 80% methanol for about 24 h. The sensitivity to microorganisms analysis was performed by the microdilution technique. Antioxidant tests were performed by scavenging the DPPH and ABTS radicals, and by FRAP assay. Anti-inflammatory activity was evaluated through the inhibition of nitric oxide production in activated macrophage RAW 264.7 cells. Cell viability was assessed with an MTT assay. Results show that the flower extract revealed a powerful antimicrobial capacity against Gram-positive bacteria and strong antioxidant and anti-inflammatory activities. No significant cytotoxicity to activated macrophages was recorded. High resolution electrospray ionization mass spectrometry and nuclear magnetic resonance analysis identified two molecules with important anti-inflammatory effects: 12α-hydroxydaturametelin B and daturametelin B. Molecular docking analysis with both pro-inflammatory agents tumor necrosis factor alpha and interleukin-6 revealed that both compounds showed good binding features with the selected target proteins. Our results suggest that D. stramonium flower is a promising source of compounds with potential antioxidant, antibacterial, and anti-inflammatory activities. Isolated withanolide steroidal lactones from D. stramonium flower extract with promising anti-inflammatory activity have therapeutic potential against inflammatory disorders. MDPI 2023-07-04 /pmc/articles/PMC10343631/ /pubmed/37446858 http://dx.doi.org/10.3390/molecules28135195 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Damergi, Bilel Essid, Rym Fares, Nadia Khadraoui, Nadine Ageitos, Lucía Ben Alaya, Ameni Gharbi, Dorra Abid, Islem Rashed Alothman, Monerah Limam, Ferid Rodríguez, Jaime Jiménez, Carlos Tabbene, Olfa Datura stramonium Flowers as a Potential Natural Resource of Bioactive Molecules: Identification of Anti-Inflammatory Agents and Molecular Docking Analysis |
title | Datura stramonium Flowers as a Potential Natural Resource of Bioactive Molecules: Identification of Anti-Inflammatory Agents and Molecular Docking Analysis |
title_full | Datura stramonium Flowers as a Potential Natural Resource of Bioactive Molecules: Identification of Anti-Inflammatory Agents and Molecular Docking Analysis |
title_fullStr | Datura stramonium Flowers as a Potential Natural Resource of Bioactive Molecules: Identification of Anti-Inflammatory Agents and Molecular Docking Analysis |
title_full_unstemmed | Datura stramonium Flowers as a Potential Natural Resource of Bioactive Molecules: Identification of Anti-Inflammatory Agents and Molecular Docking Analysis |
title_short | Datura stramonium Flowers as a Potential Natural Resource of Bioactive Molecules: Identification of Anti-Inflammatory Agents and Molecular Docking Analysis |
title_sort | datura stramonium flowers as a potential natural resource of bioactive molecules: identification of anti-inflammatory agents and molecular docking analysis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10343631/ https://www.ncbi.nlm.nih.gov/pubmed/37446858 http://dx.doi.org/10.3390/molecules28135195 |
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