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Specific host metabolite and gut microbiome alterations are associated with bone loss during spaceflight

Understanding the axis of the human microbiome and physiological homeostasis is an essential task in managing deep-space-travel-associated health risks. The NASA-led Rodent Research 5 mission enabled an ancillary investigation of the gut microbiome, varying exposure to microgravity (flight) relative...

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Autores principales: Bedree, Joseph K., Kerns, Kristopher, Chen, Tsute, Lima, Bruno P., Liu, Guo, Ha, Pin, Shi, Jiayu, Pan, Hsin Chuan, Kim, Jong Kil, Tran, Luan, Minot, Samuel S., Hendrickson, Erik L., Lamont, Eleanor I., Schulte, Fabian, Hardt, Markus, Stephens, Danielle, Patel, Michele, Kokaras, Alexis, Stodieck, Louis, Shirazi-Fard, Yasaman, Wu, Benjamin, Kwak, Jin Hee, Ting, Kang, Soo, Chia, McLean, Jeffrey S., He, Xuesong, Shi, Wenyuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10344367/
https://www.ncbi.nlm.nih.gov/pubmed/37080202
http://dx.doi.org/10.1016/j.celrep.2023.112299
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author Bedree, Joseph K.
Kerns, Kristopher
Chen, Tsute
Lima, Bruno P.
Liu, Guo
Ha, Pin
Shi, Jiayu
Pan, Hsin Chuan
Kim, Jong Kil
Tran, Luan
Minot, Samuel S.
Hendrickson, Erik L.
Lamont, Eleanor I.
Schulte, Fabian
Hardt, Markus
Stephens, Danielle
Patel, Michele
Kokaras, Alexis
Stodieck, Louis
Shirazi-Fard, Yasaman
Wu, Benjamin
Kwak, Jin Hee
Ting, Kang
Soo, Chia
McLean, Jeffrey S.
He, Xuesong
Shi, Wenyuan
author_facet Bedree, Joseph K.
Kerns, Kristopher
Chen, Tsute
Lima, Bruno P.
Liu, Guo
Ha, Pin
Shi, Jiayu
Pan, Hsin Chuan
Kim, Jong Kil
Tran, Luan
Minot, Samuel S.
Hendrickson, Erik L.
Lamont, Eleanor I.
Schulte, Fabian
Hardt, Markus
Stephens, Danielle
Patel, Michele
Kokaras, Alexis
Stodieck, Louis
Shirazi-Fard, Yasaman
Wu, Benjamin
Kwak, Jin Hee
Ting, Kang
Soo, Chia
McLean, Jeffrey S.
He, Xuesong
Shi, Wenyuan
author_sort Bedree, Joseph K.
collection PubMed
description Understanding the axis of the human microbiome and physiological homeostasis is an essential task in managing deep-space-travel-associated health risks. The NASA-led Rodent Research 5 mission enabled an ancillary investigation of the gut microbiome, varying exposure to microgravity (flight) relative to ground controls in the context of previously shown bone mineral density (BMD) loss that was observed in these flight groups. We demonstrate elevated abundance of Lactobacillus murinus and Dorea sp. during microgravity exposure relative to ground control through whole-genome sequencing and 16S rRNA analyses. Specific functionally assigned gene clusters of L. murinus and Dorea sp. capable of producing metabolites, lactic acid, leucine/isoleucine, and glutathione are enriched. These metabolites are elevated in the microgravity-exposed host serum as shown by liquid chromatography-tandem mass spectrometry (LC-MS/MS) metabolomic analysis. Along with BMD loss, ELISA reveals increases in osteocalcin and reductions in tartrate-resistant acid phosphatase 5b signifying additional loss of bone homeostasis in flight.
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spelling pubmed-103443672023-07-13 Specific host metabolite and gut microbiome alterations are associated with bone loss during spaceflight Bedree, Joseph K. Kerns, Kristopher Chen, Tsute Lima, Bruno P. Liu, Guo Ha, Pin Shi, Jiayu Pan, Hsin Chuan Kim, Jong Kil Tran, Luan Minot, Samuel S. Hendrickson, Erik L. Lamont, Eleanor I. Schulte, Fabian Hardt, Markus Stephens, Danielle Patel, Michele Kokaras, Alexis Stodieck, Louis Shirazi-Fard, Yasaman Wu, Benjamin Kwak, Jin Hee Ting, Kang Soo, Chia McLean, Jeffrey S. He, Xuesong Shi, Wenyuan Cell Rep Article Understanding the axis of the human microbiome and physiological homeostasis is an essential task in managing deep-space-travel-associated health risks. The NASA-led Rodent Research 5 mission enabled an ancillary investigation of the gut microbiome, varying exposure to microgravity (flight) relative to ground controls in the context of previously shown bone mineral density (BMD) loss that was observed in these flight groups. We demonstrate elevated abundance of Lactobacillus murinus and Dorea sp. during microgravity exposure relative to ground control through whole-genome sequencing and 16S rRNA analyses. Specific functionally assigned gene clusters of L. murinus and Dorea sp. capable of producing metabolites, lactic acid, leucine/isoleucine, and glutathione are enriched. These metabolites are elevated in the microgravity-exposed host serum as shown by liquid chromatography-tandem mass spectrometry (LC-MS/MS) metabolomic analysis. Along with BMD loss, ELISA reveals increases in osteocalcin and reductions in tartrate-resistant acid phosphatase 5b signifying additional loss of bone homeostasis in flight. 2023-05-30 2023-04-19 /pmc/articles/PMC10344367/ /pubmed/37080202 http://dx.doi.org/10.1016/j.celrep.2023.112299 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ).
spellingShingle Article
Bedree, Joseph K.
Kerns, Kristopher
Chen, Tsute
Lima, Bruno P.
Liu, Guo
Ha, Pin
Shi, Jiayu
Pan, Hsin Chuan
Kim, Jong Kil
Tran, Luan
Minot, Samuel S.
Hendrickson, Erik L.
Lamont, Eleanor I.
Schulte, Fabian
Hardt, Markus
Stephens, Danielle
Patel, Michele
Kokaras, Alexis
Stodieck, Louis
Shirazi-Fard, Yasaman
Wu, Benjamin
Kwak, Jin Hee
Ting, Kang
Soo, Chia
McLean, Jeffrey S.
He, Xuesong
Shi, Wenyuan
Specific host metabolite and gut microbiome alterations are associated with bone loss during spaceflight
title Specific host metabolite and gut microbiome alterations are associated with bone loss during spaceflight
title_full Specific host metabolite and gut microbiome alterations are associated with bone loss during spaceflight
title_fullStr Specific host metabolite and gut microbiome alterations are associated with bone loss during spaceflight
title_full_unstemmed Specific host metabolite and gut microbiome alterations are associated with bone loss during spaceflight
title_short Specific host metabolite and gut microbiome alterations are associated with bone loss during spaceflight
title_sort specific host metabolite and gut microbiome alterations are associated with bone loss during spaceflight
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10344367/
https://www.ncbi.nlm.nih.gov/pubmed/37080202
http://dx.doi.org/10.1016/j.celrep.2023.112299
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