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A novel molecular mechanism mediated by circCCDC134 regulates non‐small cell lung cancer progression

BACKGROUND: Circular RNA (circRNA) plays a crucial role in non‐small cell lung cancer (NSCLC) progression. However, the role of circCCDC134 in NSCLC is still largely unknown. METHODS: Quantitative real‐time PCR was utilized for measuring circCCDC134, microRNA (miR)‐625‐5p and nuclear factor of activ...

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Autores principales: Tong, Zhufeng, Wang, Zhen, Jiang, Jinghan, Tong, Chengcheng, Wu, Lan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons Australia, Ltd 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10344740/
https://www.ncbi.nlm.nih.gov/pubmed/37231545
http://dx.doi.org/10.1111/1759-7714.14942
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author Tong, Zhufeng
Wang, Zhen
Jiang, Jinghan
Tong, Chengcheng
Wu, Lan
author_facet Tong, Zhufeng
Wang, Zhen
Jiang, Jinghan
Tong, Chengcheng
Wu, Lan
author_sort Tong, Zhufeng
collection PubMed
description BACKGROUND: Circular RNA (circRNA) plays a crucial role in non‐small cell lung cancer (NSCLC) progression. However, the role of circCCDC134 in NSCLC is still largely unknown. METHODS: Quantitative real‐time PCR was utilized for measuring circCCDC134, microRNA (miR)‐625‐5p and nuclear factor of activated T cell 5 (NFAT5) expression. Cell functions were evaluated by colony formation, EdU, transwell, and wound healing assays and flow cytometry. Glucose consumption, lactate production, and ATP level were determined to analyze cell glycolysis. Western blot analysis was used to detect protein expression. Animal experiments were performed to assess the effect of circCCDC134 on NSCLC tumor growth. RNA interaction was evaluated by dual‐luciferase reporter assay and RIP assay. Exosomes were isolated from the serum of NSCLC patients and healthy normal controls. RESULTS: Highly expressed circCCDC134 was found in NSCLC tissues and cells, as well as in the serum exosomes of NSCLC patients. Downregulated circCCDC134 restrained NSCLC cell growth, metastasis and glycolysis. CircCCDC134 sponged miR‐625‐5p to regulate NFAT5. MiR‐625‐5p inhibitor abolished the regulation of circCCDC134 knockdown on NSCLC progression, and NFAT5 overexpression eliminated the effects of miR‐625‐5p on NSCLC cell behaviors. CircCCDC134 knockdown also inhibited NSCLC tumor growth. CONCLUSION: Our study revealed that circCCDC134 was involved in regulating NSCLC progression through the miR‐625‐5p/NFAT5 pathway, confirming that circCCDC134 might function as the diagnostic and therapeutic target for NSCLC.
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spelling pubmed-103447402023-07-15 A novel molecular mechanism mediated by circCCDC134 regulates non‐small cell lung cancer progression Tong, Zhufeng Wang, Zhen Jiang, Jinghan Tong, Chengcheng Wu, Lan Thorac Cancer Original Articles BACKGROUND: Circular RNA (circRNA) plays a crucial role in non‐small cell lung cancer (NSCLC) progression. However, the role of circCCDC134 in NSCLC is still largely unknown. METHODS: Quantitative real‐time PCR was utilized for measuring circCCDC134, microRNA (miR)‐625‐5p and nuclear factor of activated T cell 5 (NFAT5) expression. Cell functions were evaluated by colony formation, EdU, transwell, and wound healing assays and flow cytometry. Glucose consumption, lactate production, and ATP level were determined to analyze cell glycolysis. Western blot analysis was used to detect protein expression. Animal experiments were performed to assess the effect of circCCDC134 on NSCLC tumor growth. RNA interaction was evaluated by dual‐luciferase reporter assay and RIP assay. Exosomes were isolated from the serum of NSCLC patients and healthy normal controls. RESULTS: Highly expressed circCCDC134 was found in NSCLC tissues and cells, as well as in the serum exosomes of NSCLC patients. Downregulated circCCDC134 restrained NSCLC cell growth, metastasis and glycolysis. CircCCDC134 sponged miR‐625‐5p to regulate NFAT5. MiR‐625‐5p inhibitor abolished the regulation of circCCDC134 knockdown on NSCLC progression, and NFAT5 overexpression eliminated the effects of miR‐625‐5p on NSCLC cell behaviors. CircCCDC134 knockdown also inhibited NSCLC tumor growth. CONCLUSION: Our study revealed that circCCDC134 was involved in regulating NSCLC progression through the miR‐625‐5p/NFAT5 pathway, confirming that circCCDC134 might function as the diagnostic and therapeutic target for NSCLC. John Wiley & Sons Australia, Ltd 2023-05-25 /pmc/articles/PMC10344740/ /pubmed/37231545 http://dx.doi.org/10.1111/1759-7714.14942 Text en © 2023 The Authors. Thoracic Cancer published by China Lung Oncology Group and John Wiley & Sons Australia, Ltd. https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Original Articles
Tong, Zhufeng
Wang, Zhen
Jiang, Jinghan
Tong, Chengcheng
Wu, Lan
A novel molecular mechanism mediated by circCCDC134 regulates non‐small cell lung cancer progression
title A novel molecular mechanism mediated by circCCDC134 regulates non‐small cell lung cancer progression
title_full A novel molecular mechanism mediated by circCCDC134 regulates non‐small cell lung cancer progression
title_fullStr A novel molecular mechanism mediated by circCCDC134 regulates non‐small cell lung cancer progression
title_full_unstemmed A novel molecular mechanism mediated by circCCDC134 regulates non‐small cell lung cancer progression
title_short A novel molecular mechanism mediated by circCCDC134 regulates non‐small cell lung cancer progression
title_sort novel molecular mechanism mediated by circccdc134 regulates non‐small cell lung cancer progression
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10344740/
https://www.ncbi.nlm.nih.gov/pubmed/37231545
http://dx.doi.org/10.1111/1759-7714.14942
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