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Oxytetracycline-induced inflammatory process without oxidative stress in blue mussels Mytilus trossulus

Potentially harmful compounds including pharmaceuticals are commonly found in marine waters and sediments. Amongst those, antibiotics and their metabolites are detected worldwide in various abiotic (at concentrations as high as µg/L) and biotic matrices at ng/gram of tissue, posing a risk to non-tar...

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Autores principales: Hallmann, Anna, Leszczyńska, Dagmara, Czumaj, Aleksandra, Świeżak, Justyna, Caban, Magda, Michnowska, Alicja, Smolarz, Katarzyna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10345040/
https://www.ncbi.nlm.nih.gov/pubmed/37301807
http://dx.doi.org/10.1007/s11356-023-28057-z
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author Hallmann, Anna
Leszczyńska, Dagmara
Czumaj, Aleksandra
Świeżak, Justyna
Caban, Magda
Michnowska, Alicja
Smolarz, Katarzyna
author_facet Hallmann, Anna
Leszczyńska, Dagmara
Czumaj, Aleksandra
Świeżak, Justyna
Caban, Magda
Michnowska, Alicja
Smolarz, Katarzyna
author_sort Hallmann, Anna
collection PubMed
description Potentially harmful compounds including pharmaceuticals are commonly found in marine waters and sediments. Amongst those, antibiotics and their metabolites are detected worldwide in various abiotic (at concentrations as high as µg/L) and biotic matrices at ng/gram of tissue, posing a risk to non-target species exposed to them such as blue mussels. Amongst those, oxytetracycline (OTC) belongs to the most detected antibiotics in the marine environment. In this work, we concentrated on studying the potential induction of oxidative stress, activation of cellular detoxification processes (including Phase I and Phase II xenobiotic biotransformation enzymes) and multixenobiotic resistance pumps (Phase III) as well as changes in the aromatisation efficiency in Mytilus trossulus exposed to 100 μg/L OTC. Our results show that 100 µg/L OTC concentration did not provoke cellular oxidative stress and did not affect the expression of genes involved in detoxification processes in our model. Moreover, no effect of OTC on aromatisation efficiency was found. Instead, phenoloxidase activity measured in haemolymph was significantly higher in OTC exposed mussels than in those from the control (30.95 ± 3.33 U/L and 17.95 ± 2.75 U/L, respectively). OTC exposed mussels were also characterised by a tissue-dependant activation of major vault protein (MVP) gene expression (1.5 times higher in gills and 2.4 times higher in the digestive system) and a decreased expression of the nuclear factor kappa B-a (NF-κB) gene (3.4 times lower in the digestive system) when compared to those from the control. Additionally, an elevated number of regressive changes and inflammatory responses in tissues such as gills, digestive system and mantle (gonads) was observed underlining the worsening of bivalves’ general health. Therefore, instead of a free-radical effect of OTC, we for the first time describe the occurrence of typical changes resulting from antibiotic therapy in non-target organisms like M. trossulus exposed to antibiotics such as OTC. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11356-023-28057-z.
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spelling pubmed-103450402023-07-15 Oxytetracycline-induced inflammatory process without oxidative stress in blue mussels Mytilus trossulus Hallmann, Anna Leszczyńska, Dagmara Czumaj, Aleksandra Świeżak, Justyna Caban, Magda Michnowska, Alicja Smolarz, Katarzyna Environ Sci Pollut Res Int Research Article Potentially harmful compounds including pharmaceuticals are commonly found in marine waters and sediments. Amongst those, antibiotics and their metabolites are detected worldwide in various abiotic (at concentrations as high as µg/L) and biotic matrices at ng/gram of tissue, posing a risk to non-target species exposed to them such as blue mussels. Amongst those, oxytetracycline (OTC) belongs to the most detected antibiotics in the marine environment. In this work, we concentrated on studying the potential induction of oxidative stress, activation of cellular detoxification processes (including Phase I and Phase II xenobiotic biotransformation enzymes) and multixenobiotic resistance pumps (Phase III) as well as changes in the aromatisation efficiency in Mytilus trossulus exposed to 100 μg/L OTC. Our results show that 100 µg/L OTC concentration did not provoke cellular oxidative stress and did not affect the expression of genes involved in detoxification processes in our model. Moreover, no effect of OTC on aromatisation efficiency was found. Instead, phenoloxidase activity measured in haemolymph was significantly higher in OTC exposed mussels than in those from the control (30.95 ± 3.33 U/L and 17.95 ± 2.75 U/L, respectively). OTC exposed mussels were also characterised by a tissue-dependant activation of major vault protein (MVP) gene expression (1.5 times higher in gills and 2.4 times higher in the digestive system) and a decreased expression of the nuclear factor kappa B-a (NF-κB) gene (3.4 times lower in the digestive system) when compared to those from the control. Additionally, an elevated number of regressive changes and inflammatory responses in tissues such as gills, digestive system and mantle (gonads) was observed underlining the worsening of bivalves’ general health. Therefore, instead of a free-radical effect of OTC, we for the first time describe the occurrence of typical changes resulting from antibiotic therapy in non-target organisms like M. trossulus exposed to antibiotics such as OTC. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11356-023-28057-z. Springer Berlin Heidelberg 2023-06-10 2023 /pmc/articles/PMC10345040/ /pubmed/37301807 http://dx.doi.org/10.1007/s11356-023-28057-z Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Hallmann, Anna
Leszczyńska, Dagmara
Czumaj, Aleksandra
Świeżak, Justyna
Caban, Magda
Michnowska, Alicja
Smolarz, Katarzyna
Oxytetracycline-induced inflammatory process without oxidative stress in blue mussels Mytilus trossulus
title Oxytetracycline-induced inflammatory process without oxidative stress in blue mussels Mytilus trossulus
title_full Oxytetracycline-induced inflammatory process without oxidative stress in blue mussels Mytilus trossulus
title_fullStr Oxytetracycline-induced inflammatory process without oxidative stress in blue mussels Mytilus trossulus
title_full_unstemmed Oxytetracycline-induced inflammatory process without oxidative stress in blue mussels Mytilus trossulus
title_short Oxytetracycline-induced inflammatory process without oxidative stress in blue mussels Mytilus trossulus
title_sort oxytetracycline-induced inflammatory process without oxidative stress in blue mussels mytilus trossulus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10345040/
https://www.ncbi.nlm.nih.gov/pubmed/37301807
http://dx.doi.org/10.1007/s11356-023-28057-z
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