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CRIC-seq protocol for in situ profiling of proximal RNA-RNA contacts associated with RNA-binding proteins

RNA-binding proteins (RBPs) can bind and mediate RNA-RNA contacts. However, identifying specific RBP-organized RNA-RNA contacts remains challenging. Here, we present a capture RIC-seq (CRIC-seq) technique to map specific RBP-associated RNA-RNA contacts globally. We describe steps for formaldehyde cr...

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Detalles Bibliográficos
Autores principales: Ye, Rong, Hu, Naijing, Xue, Yuanchao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10345188/
https://www.ncbi.nlm.nih.gov/pubmed/37405924
http://dx.doi.org/10.1016/j.xpro.2023.102401